Banca de QUALIFICAÇÃO: LEONARDO DE MEDEIROS AQUINO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : LEONARDO DE MEDEIROS AQUINO
DATE: 09/04/2025
TIME: 08:00
LOCAL: Videoconferência - Link para acesso: https://meet.google.com/hff-fhjx-www
TITLE:

THERAPEUTIC POTENTIAL AND UNDESIRABLE EFFECTS OF CAROTENOIDS AND RETINOIDS IN UROLITHIASIS, AN IN VITRO APPROACH, AND IN SILICO ANALYSIS OF RISKS IN OTHER PHYSIOLOGICAL SYSTEMS.

KEY WORDS:

Antioxidants; Urolithiasis; Oxidative stress; Calcium oxalate crystals; In silico

PAGES: 81
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:

Beta-carotene (BCAR), astaxanthin (AST), and retinol (RET) are described as antioxidant molecules that can be used to combat damage caused by oxidative stress. Urolithiasis, the formation of kidney stones, is one of the consequences of oxidative stress. However, there is no data on how these three molecules act to prevent or specifically combat urolithiasis. In this context, this study initially evaluated in vitro the potential of BCAR, AST, and RET, known antioxidants, as inhibitors of kidney stone formation. BCAR, AST, or RET (10 and 15 µg) were not toxic to canine kidney cells (MDCK) but were also unable to protect these cells from damage caused by the presence of H2O2. Regarding the direct formation of calcium oxalate crystals, it was found that the compounds evaluated here equally affected the balance between the quantity of types of calcium oxalate crystals formed, inducing the formation of a greater quantity of COD crystals, which are more harmful. These data, although initial, demonstrate an undesirable effect not yet described for this class of molecules. Given this new context, in silico techniques were used to evaluate carotenes (BCAR and lycopene), retinoids (RET and isotretinoin), and xanthophylls (AST, fucoxanthin, zeaxanthin, beta-cryptoxanthin, canthaxanthin, and lutein) and identify other possible adverse effects. Within their respective groups, similarities can be seen in the ADMET profile of the compounds evaluated. Using the PredHerg platform, weak cardiotoxic potential was identified for lycopene, beta-cryptoxanthin, fucoxanthin, and moderate for lutein. Using the PredSkin platform, it was identified that lycopene and the tested xanthophylls, except canthaxanthin, have the potential to cause allergic skin reactions. The findings of this study suggest that although BCAR, AST, and RET are known antioxidants, they may have unintended negative effects on kidney stone formation by promoting the formation of more harmful calcium oxalate crystals, particularly COD crystals. In addition, the in silico analysis revealed potential cardiotoxicity and skin allergy risks for some of the compounds evaluated. These initial results highlight the need for further investigation into the safety and efficacy of these antioxidant molecules, especially in the context of kidney health and oxidative stress.

COMMITTEE MEMBERS:
Externo ao Programa - 3060683 - LEONARDO THIAGO DUARTE BARRETO NOBRE - UFRNExterno à Instituição - DIEGO DE ARAUJO SABRY - IFPI
Externo à Instituição - MOACIR FERNANDES DE QUEIROZ NETO - UFPB