Banca de DEFESA: ARIANA PEREIRA DA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ARIANA PEREIRA DA SILVA
DATE: 25/12/2023
TIME: 14:00
LOCAL: videoconferencia
TITLE:

PROSPECTION OF THE ANTIOXIDANT ACTIVITY IN VITRO AND IN VIVO OF CHAÑAR SEED (GEOFFROEA DECORTICANS) EXTRACTS (GILL. EX HOOK. ET ARN.) BURKART (FABACEAE).

KEY WORDS:

Ethanolic extract; Aqueous extract; Phenolic compounds; Oxidative stress; Copper sulfate; Tenebrio molitor. 

PAGES: 73
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUBÁREA: Química de Macromoléculas
SUMMARY:

The use of plants for medicinal purposes has been practiced since time immemorial. Various parts of plants are used for this purpose, and their use is as a complementary therapeutic source Geoffroea decorticans, popularly known as Chañar, is a plant native to Chile, All parts of this plant are used in folk medicine, ranging from expectorant activity, including bronchopulmonary disorders and pain relief, as well as antioxidant and antinociceptive activity. However, few studies are reporting such activities with Chañar seed extracts. That said, our work aimed to evaluate and characterize the antioxidant, cytotoxic and protective potential against oxidative stress induced by copper sulfate (CuSO4) of the ethanolic (EE) and aqueous (EA) extracts of Chañar seed using in vitro and in vivo methodologies. The two extracts produced were phytochemically characterized using HPLC - GC-MS/MS, as well as spectrophotometric tests such as total phenolic compounds and total flavonoids. Subsequently, biochemical tests were carried out to assess the in vitro antioxidant potential using five spectrophotometric tests (total antioxidant capacity-CAT, reducing power; 2,2-diphenyl-1-picrylhydrazyl-DPPH; copper and iron chelation) aimed at verifying the possible modes of action by which these bioactive compounds could be contributing to the maintenance of cellular redox homeostasis. The 3T3 ATCCCCL-92 fibroblast cell line was also used to assess the cytotoxicity of EE and EA, as well as the protective role of the extracts when this cell line was subjected to the stress agent copper sulfate (CuSO4), in addition to the potential to induce cell migration or not. After carrying out the in vivo tests, the extracts were tested on an invertebrate animal model (Tenebrio molitor). Survival tests were carried out to assess the toxicity of the extracts and their protective capacity against oxidative stress caused by copper sulfate. Melanisation parameters were also assessed. The results obtained by chromatography detected the presence of secondary metabolites such as phytol, alpha-tocopherol, vitexin and rutin. The in vitro spectrophotometric tests demonstrated the antioxidant capacity of the extracts to act by different mechanisms of action (inhibiting, sequestering and chelating). The tests on the 3T3 cell model demonstrated the absence of EE and EA cytotoxicity, also conferring protection against (CuSO4) stress and cell migration capacity. In the animal model, the extracts once again showed their excellent protective capacity against oxidative stress, capable of combating the free radicals generated, mitigating the melanization exacerbated by stress and the lack of modulation in the insect's metamorphosis process. Taken together (in vitro and in vivo), the EE and EA extracts of Chañar seeds are a source of bioactive compounds with excellent antioxidant properties.

COMMITTEE MEMBERS:
Presidente - 1453487 - KATIA CASTANHO SCORTECCI
Interno - 2962496 - RAFAEL BARROS GOMES DA CAMARA
Externo à Instituição - CARLOS HENRIQUE SALVINO GADELHA MENESES - UEPB