Banca de QUALIFICAÇÃO: PAULO EDUARDO TOSCANO SOARES

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : PAULO EDUARDO TOSCANO SOARES
DATE: 27/04/2022
TIME: 14:00
LOCAL: Videoconferência - Link para acesso: https://meet.google.com/okd-yvme-cut
TITLE:

Application of Next Generation Sequencing in Penaeus vannamei shrimp cultives from Rio Grande do Norte

KEY WORDS:

Next Generation Sequencing, Parentage Analysis, Penaeus vannamei, Genotyping

PAGES: 72
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:

The Penaeus vannamei shrimp is a culture with high aggregated value and has a great impact on aquaculture, being the most cultivated species in Brazil and in the world. To increase the production in cultives, it is necessary to manage the genetic heritage present there to avoid inbreeding and increase the frequency of individuals with characteristics of interest, such as resistance to pathogens and growth speed. For this, genotyping methods are based in parentage analyses being useful in the selection of these individuals. The methodologies currently used are still mostly based on microsatellite markers, which have a high cost of implementation and allow the genotyping of only a few individuals at a time, being also labor intensive. Here in this work, we sought to evaluate the use of SNP markers in the genotyping of shrimp from cultives. SNP markers have a high reliability due to the accuracy of the sequencing of the sequences, a lower cost per individual and are widely distributed throughout the genomes, facilitating their application in any species. We sought to incorporate mitochondrial markers in the panel because mitochondrial DNA has an inheritance system and physical characteristics different from genomic DNA. For this, the intra-individual and inter-individual variability of the mitogenome was characterized and differences in inter and intra variability were observed. The results of these analyzes showed the need for caution in choosing mitochondrial markers, which may reflect more mutations that occur only in the individual than in the population itself. Thus, mitochondrial primers were designed to capture inter-individual variability, and if possible intra-individual as well, to include in a panel of 581 genomic primers. Currently, a panel containing a selection of 104 pairs of primers (10 being mitochondrial) was prepared and is currently under analysis.

BANKING MEMBERS:
Presidente - 1046922 - LEONARDO CAPISTRANO FERREIRA
Interno - 1513597 - JOAO PAULO MATOS SANTOS LIMA
Externo à Instituição - RODOLFO LUIS PETERSEN - UFPR