Banca de DEFESA: GABRIELLA SILVA CAMPOS CARELLI

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : GABRIELLA SILVA CAMPOS CARELLI
DATE: 24/12/2021
TIME: 10:00
LOCAL: Remoto
TITLE:

INFLUENCE OF CAFFEINE ON PROTEIN PROFILE AND FEASIBILITY Escherichia coli CELL PHONE AND ADJUVANT POTENTIAL FOR ANTIBIOTICS

KEY WORDS:

Trimethylxanthine, gammaproteobacteria, secondary metabolite

PAGES: 69
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUBÁREA: Química de Macromoléculas
SPECIALTY: Proteínas
SUMMARY:

Caffeine is a substance found in over 100 species of plants. In addition to its neurostimulant effects, its antimicrobial activity has already been proven by science. In this context, the aim of this work was to test the influence of caffeine on the protein profile and cell viability of Escherichia coli and its adjuvant potential for antibiotics in vitro. Commercially acquired caffeine was solubilized in Mueller Hinton broth and distilled water, 10 mL of this solution were placed in seven (7) test tubes at different final concentrations of caffeine. After performing a concentration curve, it was evidenced that with the use of a concentration of only 0.20 mg/mL, caffeine allowed a partial bacterial growth. To perform the extraction of proteins from E. coli, two different methods were compared, one with acetone pa and the other with glass beads. By analyzing the SDS-PAGE and the densitometry of the extraction, it was concluded that the method with acetone was the most satisfactory. For the other tests, three generations of the bacteria were cultivated. Bacterial strains cultivated with caffeine showed alterations in the protein profile when compared to those cultivated in the absence of this substance, this fact was verified through SDS-PAGE and optical densitometry. After concluding that caffeine had an influence on the protein profile of the bacteria studied, its potential as an adjuvant to the antibiotics ampicillin and streptomycin was tested, the results of the adjuvant trial showed that caffeine was more effective when combined with ampicillin, reducing the inhibitory concentration minimum of this antibiotic from 0.250 mg/mL to 0.125 mg/mL. The combinations of caffeine and streptomycin were not statistically significant. Taken together, the data found suggest that caffeine, alone or in combination with ampicillin, is a potential candidate for applications in antibacterial therapies.

BANKING MEMBERS:
Externo à Instituição - ANDERSON FELIPE JÁCOME DE FRANÇA - UFRN
Interno - 1149356 - ELIZEU ANTUNES DOS SANTOS
Externo à Instituição - VINÍCIUS CAMPELO SOEIRO - FACENE