Banca de QUALIFICAÇÃO: AMAXSELL THIAGO BARROS DE SOUZA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : AMAXSELL THIAGO BARROS DE SOUZA
DATE: 25/02/2026
TIME: 16:00
LOCAL: Sala de Aulas GEP/MEJC
TITLE:

ASSOCIATION BETWEEN GENETIC VARIANTS IN MICRORNAS AND LONG NON-CODING RNAS AND SUSCEPTIBILITY TO RECURRENT PREGNANCY LOSS

KEY WORDS:

MicroRNAs; RNA, Long Noncoding; Polymorphism, Single Nucleotide; Abortion, Habitual.

PAGES: 50
BIG AREA: Ciências da Saúde
AREA: Saúde Coletiva
SUMMARY:

Recurrent pregnancy loss (RPL) represents one of the most prevalent complications of early pregnancy and remains without a clearly defined etiology in approximately 50-60% of cases. Recent scientific evidence indicates that post-transcriptional regulatory mechanisms mediated by non-coding RNAs, particularly microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), play a critical role at the maternal–embryonic interface, influencing key biological processes such as embryo implantation, placentation, angiogenesis, and modulation of the maternal immune response. Single nucleotide polymorphisms (SNPs) located within genes encoding these regulatory RNAs may impair their biogenesis, stability, or molecular function, thereby contributing to susceptibility to RPL. The aim of this study was to investigate the association between SNPs in miRNA and lncRNA genes and the risk of RPL through two systematic reviews with meta-analysis, prospectively registered in PROSPERO (CRD420251276100 and CRD420261298804) and conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta- Analyses (PRISMA) and the Meta-analysis of Observational Studies in Epidemiology (MOOSE) guidelines. Bibliographic searches were performed in the PubMed, Embase, Web of Science, and Scopus databases, covering publications up to January 2026. Data extraction was carried out independently by two reviewers, and methodological quality was assessed using the Newcastle–Ottawa Scale. Quantitative synthesis was performed using Review Manager (RevMan) version 5.4, and the certainty of the evidence was evaluated according to the Grading of Recommendations, Assessment, Development and Evaluation (GRADE) approach. In the first study, 29 studies comprising 11,088 participants (5,855 cases and 5,233 controls) were included. Meta-analysis revealed statistically significant associations between RPL and the variants miR-10a (rs3809783), miR-149 (rs2292832), miR- 323b (rs56103835), miR-449b (rs10061133), and miR-604 (rs2368393), with genotype-specific effects conferring either increased risk or protection. In the second study, which included 16 studies and 11,623 participants (5,169 cases and 6,454 controls), variants in the lncRNAs HOTAIR (rs920778, rs1899663, and rs4759314) and HOTTIP (rs1859168) were significantly associated with susceptibility to RPL. The overall certainty of the evidence was rated as very low, primarily due to imprecision, inconsistency across studies, and methodological limitations inherent to observational study designs. Nevertheless, the findings of this thesis provide consistent evidence that genetic variants in non-coding RNAs are implicated in the pathophysiology of recurrent pregnancy loss. These results underscore the need for future multicenter studies with greater statistical power and integrated functional approaches to validate these associations and elucidate their underlying biological mechanisms.

COMMITTEE MEMBERS:
Interna - 1714243 - DANIELLA REGINA ARANTES MARTINS SALHA
Externo à Instituição - KLEYTON SANTOS DE MEDEIROS
Presidente - 1674709 - VIVIANE SOUZA DO AMARAL