Banca de QUALIFICAÇÃO: CAIO RODRIGUES MAIA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : CAIO RODRIGUES MAIA
DATE: 25/07/2025
TIME: 14:00
LOCAL: Auditório do Departamento de Morfologia (CB) - UFRN
TITLE:

MORPHOLOGICAL, IMMUNOHISTOCHEMICAL AND IMMUNOFLUORESCENCE ANALYSIS OF CHEMICALLY INDUCED TONGUE AND PALATE SQUAMOID CARCINOMAS IN RATS.

KEY WORDS:

Oral Squamous Cell Carcinomas; Carcinogenesis; Wistar Rats; Band 4.1-Ezrin-Radixin-Moesin Domain; Fluorescence Microscopy.

PAGES: 45
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUMMARY:

Oral squamous cell carcinoma (OSC) is the most common malignant neoplasm affecting the head and neck, with a frequency of over 300,000 cases in 2020. Furthermore, it has a high mortality rate, primarily due to late diagnosis and, coupled with the variability of molecular mechanisms that characterize this lesion, constituting a significant public health challenge that must be addressed. Several markers that are altered in OS are being studied for prognostic prediction, providing a basis for therapeutic targeting. Ezrin, NF2/Merlin, and BAP-1 are three of the markers emerging as potential predictors in OS because they are proteins known for tumor suppression and are altered in several malignant neoplasms of epithelial origin, including OS, as is the case with Ezrin. Thus, the objective of this study will be to analyze the macroscopic and histopathological aspects of cases of squamous cell carcinoma of the tongue and palate chemically induced by 4NQO in rats, through the analysis of the depth of tumor invasion, immunohistochemical analysis of BAP-1 and NF2/Merlin, in addition to the immunofluorescence analysis of Erzin. This study will use lesions induced in male Wistar rats (n = 50) by the carcinogen 4NQO administered systemically in their water supply. These lesions will be euthanized at different weeks of induction. After sample collection, the specimens will undergo macroscopic analysis, immunohistochemistry for BAP-1 and NF2/Merlin, immunofluorescence analysis for Ezrin, and depth of invasion, comparing lesions present on the tongue and palate of the rats. Given the above, we hope to contribute to the understanding of the behavior and progression of these neoplasms at different sites with progressive phases of chemical carcinogenesis induction in rats.

COMMITTEE MEMBERS:
Presidente - 2859541 - PEDRO PAULO DE ANDRADE SANTOS
Interna - 2374605 - AURIGENA ANTUNES DE ARAUJO
Interno - 2220417 - CARLOS AUGUSTO GALVAO BARBOZA