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Dissertations |
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1
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MARIA EDUARDA LIMA DO NASCIMENTO MARINHO
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IMPACT OF THE BIOMODIFICATION WITH SCHINOPSIS BRASILIENSIS (BRAÚNA) STEM EXTRACT ON THE ULTIMATE TENSILE STRENGTH OF A RESIN-BASED SEALANT
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Advisor : BONIEK CASTILLO DUTRA BORGES
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COMMITTEE MEMBERS :
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BONIEK CASTILLO DUTRA BORGES
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DÉBORA MICHELLE GONÇALVES DE AMORIM
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LETICIA VIRGINIA DE FREITAS CHAVES
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Data: Mar 7, 2022
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Show Abstract
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Introduction: resin-based sealants have a high success rate in preventing dental caries, but they do not have antibacterial activity, so that a high incidence of caries lesions is observed, which negatively impacts on health quality. The biomodification with the Schinopsis brasiliensis (Braúna) stem extract may be an alternative to improve its clinical characteristics. Objective: To evaluate if the biomodification of the Fluroshield® sealant with different amounts of Schinopsis brasiliensis affects its ultimate tensile strength. Methods: the extract was weighed on an analytical balance and mixed with the sealant in different concentrations: 0 mg/mL (control), 2 mg/mL, 0.5 mg/mL and 0.125 mg/mL by means of a centrifugal machine. Bar-shaped specimens with dimensions of 8 mm x 2 mm x 1 mm were prepared (n=3). The ultimate tensile strength was measured using a microtensile machine (OM100). Before testing, the sectional area (mm2) was obtained with a caliper. Data were obtained in Kgf and transformed into MPa. Statistical analysis was performed using the Kruskal-Wallis test (p<0.05). Results: The maximum tensile strength in median (minimum-maximum) of the 0 mg/mL (control), 2 mg/mL, 0.5 mg/mL and 0.125 mg/mL groups was, respectively, 56 (29-56), 53 (45-60), 48 (46-62) and 61 (38-64). There were no statistically significant differences among the groups (p=0.8). Conclusion: the addition of Schinopsis brasiliensis stem extract did not decrease the ultimate tensile strength of the Fluroshield® sealant.
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2
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JABES GENNEDYR DA CRUZ LIMA
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CLINICAL-DEMOGRAPHIC EVALUATION OF PATIENTS WITH DIABETES MELLITUS, SYSTEMIC ARTERIAL HYPERTENSION WITH SECONDARY BURNING ORAL DIAGNOSIS: A CASE-CONTROL STUDY
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Advisor : PATRICIA TEIXEIRA DE OLIVEIRA
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COMMITTEE MEMBERS :
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PATRICIA TEIXEIRA DE OLIVEIRA
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AMANDA KATARINNY GOES GONZAGA
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GLÓRIA MARIA DE FRANÇA
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Data: Jun 4, 2022
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Show Abstract
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Burning Mouth (ABS) can present a systemic picture, a differential diagnosis, presented and Secondary of the oral mucosa should be included in the systemic picture of Burning Mouth (SAB). The purpose of this study was to investigate whether there is an association between the complaint of Secondary Burning Mouth, type II DM and SAH, in addition to the use of medications for such conditions. It was characterized as a retrospective, case-control, consisting of 102 patients with ABS with SAH or type II DM, and a control group consisting of 102 patients studied by age and sex, with SAH or type II DM and without complaints of type II burning and without complaints of type II burning and without complaints of type II burning. (Comparison between groups and Pearson's chi-square test (χ2) and Fisher's exact tests were used, in addition to the 95% odds ratio (OR) test. The multivariate logistic model was also applied, for (of the adjusted odds ratio). After analysis, gastritis was identified as a risk factor for ABS (Ora=2,50; 95% CI = 1.3–4.74; p=0.005). The use of antihypertensive subclasses, such as beta-blockers (ORa = 0.36; 95% CI = 0.16-0.80; p=0.012), angiotensin-converting enzyme inhibitors (ORa = 0.19; CI 95% = 0.06-0.56; p=0.003) and angiotensin receptor blockers (ORa = 0.26; 95% CI = 0.14-0.49; p<0.001) proved to be factors protection for the ABS.
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3
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CECÍLIA SANTOS GALVÃO
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CLINICAL AND LABORATORY CONFECTION TIME OF CONVENTIONAL X MILLED OCCLUSAL SPLINT IN PATIENTS WITH BRUXISM: CLINICAL TRIAL
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Advisor : ERIKA OLIVEIRA DE ALMEIDA FREITAS
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COMMITTEE MEMBERS :
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ERIKA OLIVEIRA DE ALMEIDA FREITAS
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LAERCIO ALMEIDA DE MELO
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Paulo Cézar Simamoto Júnior
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Data: Jul 26, 2022
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Show Abstract
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The aim of the study was to compare the clinical and laboratory preparation time and clinical adjustments for the installation of conventional thermopolymerizable resin splint (CS) and milled CAD/CAM (MS) occlusal splints in patients with sleep bruxism. For this purpose, a controlled and non-randomized clinical trial was carried out in which patients diagnosed with probable sleep bruxism were included. CS (N= 24) and MS (N= 24) were prepared and the clinical preparation time (CPT), laboratory preparation time (LPT) and installation time (IT) were measured for each of the splints. The Mann-whitney test was used in the SPSS IBM Statistics program to analyze whether there is a difference between the analyzed times and the type splint performed. As a result, it was found that CS had higher total CPT (mean of 38,03 min) in relation to MS (17,90 min) (p0<001). However, the total LPT of the CS was lower (164,84 min) than the MS (185,36min) (p=0.015). For the IT, it was found that the MS had an average of 22,83min, while the CS had 37,19min (p0<001). In conclusion, within the limitations of the study, it was observed that the milled splint had a shorter clinical time both in the manufacturing and installation stages. However, the laboratory preparation time of the conventional splint was shorter.
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4
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JULLIANY TAVERNY SOUSA
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Immunoexpression of ING3 and cyclin D1 proteins between epithelial dysplasias and epidermoid carcinomas of the tongue.
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Advisor : LEAO PEREIRA PINTO
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COMMITTEE MEMBERS :
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KEILA MARTHA AMORIM BARROSO
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LEAO PEREIRA PINTO
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LELIA MARIA GUEDES QUEIROZ
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Data: Jul 28, 2022
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Show Abstract
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Oral squamous cell carcinoma (OSCC) is a malignant neoplasm of complex pathogenesis. In turn, oral epithelial dysplasia (OED) is an entity that, depending on its evolution, exhibits varied risks of malignant transformation to OSCC. The altered expression of tumor suppressor genes and cell cycle regulatory proteins are related to the etiopathogenesis of different malignant tumors. Among the tumor suppressor genes, the family of inhibitors of growth (ING) stands out, which prevents uncontrolled cell formation. Amid the cell cycle regulatory proteins, Cyclin D1 acts in the progression from S to G1 phase, allowing cell proliferation. The aim of the present study was to analyze the immunoexpression of ING3 and Cyclin D1 proteins in 28 OED and 25 OSCC specimens, located on the tongue, as well as morphologically evaluate and reclassify the lesions according to the criteria established by the World Health Organization (WHO) and by Kujan et al. (2006), for OED; by Almagunsh et al. (2015) for OSCC. Protein immunoexpression was quantitatively evaluated, considering the cellular location (cytoplasmic and/or nuclear for ING3 and nuclear for Cyclin D1), present in the epithelial component. Protein expression was compared between the two groups of samples, as well as with the clinical-pathological parameters of the lesions studied, through the Mann-Whitney (U) statistical tests and the Spearman correlation test, adopting a significance level of 5% for all tests considering the value of p≤ 0.05. Of the 28 cases of OED analyzed, 53.6% were high-risk lesions according to the binary model and of the 25 cases of OSCC analyzed, 48% were considered high-risk scores according to the BD model. No statistically significant associations were found between the morphological variables of the lesions studied and the clinical characteristics (p>0.05). A statistically significant difference was observed between the absence of ING3 expression and the grading of OEDs, according to the WHO (p<0.05). Regarding the expression of Cyclin D1, it was significantly higher in OED, when compared to OSCC cases (p<0.05). Nucleocytoplasmic ING3 expression was significantly lower in OSCC cases when compared to OED cases (p<0.05). Cytoplasm-restricted ING3 expression was higher in OED cases when compared to OSCC cases (p<0.05). The results of the present study suggest that, although there is no evidence of a significant relationship between the expression of proteins and the histopathological gradations of the lesions, there is a notable decrease in the nuclear expression of ING3, with an increase in the severity of the lesions, indicating an altered tumor suppressor function. of this protein in OED and OSCCs. We also highlight the increased expression of Cyclin D1 in OEDs, showing that these lesions have a significant rate of cell proliferation.
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5
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KATRYNE TARGINO RODRIGUES
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EVALUATION OF THE PROBIOTIC LACTOBACILLUS RHAMNOSUS EM1107 ADJUNCT TO PERIODONTAL THERAPY IN EXPERIMENTAL MODEL OF PERIODONTITIS
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Advisor : ANA RAFAELA LUZ DE AQUINO MARTINS
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COMMITTEE MEMBERS :
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ANA RAFAELA LUZ DE AQUINO MARTINS
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BRUNO CESAR DE VASCONCELOS GURGEL
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RACHEL DE QUEIROZ FERREIRA RODRIGUES
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Data: Jul 28, 2022
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Show Abstract
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Periodontal disease (PD) is characterized by a host-mediated immune-inflammatory response associated with pathogens, which compromises the protective and supporting tissues of the periodontium. It is commonly treated through non-surgical periodontal therapy in the form of scaling and root surface planing (SRP). However, in some situations, such as in the control of deep periodontal pockets, which are difficult to access and with destruction and progression of the disease over time, the practice of adjuvant therapies is indicated. In this sense, the aim of this study is to evaluate the anti-oxidant and anti-inflammatory effects of the administration of the probiotic Lactobacillus rhamnosus EM1107 (LR) as an adjunct to the basic periodontal treatment of ligature-induced periodontitis in mice. For the study, the periodontal disease induction model was used by placing a 4.0 silk ligature around the upper left second molar of mice. The animals were divided into four groups: Group I: - PD – LR - RAR; Group II: + PD – LR - RAR; Group III: + PD – LR + RAR; Group IV: + PD + LR + RAR. Biochemical analyzes of liver and kidney functions, leukocyte count, intestinal tissue concentration of glutathione (GSH), malondialdehyde (MDA) and superoxide dismutase (SOD) and expression of blood cytokines IL-1β and IL-6 were performed. The analysis of biochemical parameters revealed values above the standards in the levels of TGO/AST for groups II, III and IV. All groups maintained TGP/ALT, urea and creatinine levels within reference values. The number of leukocytes was also within the reference standards for all groups. No statistically significant differences were found between groups for tissue levels of GSH and SOD. For MDA, an increase in levels was observed in relation to the PD + RACR group, whose difference was statistically significant in relation to the positive control and negative control groups (p<0.05). There was no statistically significant difference in the concentration of inflammatory cytokines IL-1β and IL-6, despite a reduction in tissue concentrations of IL-1β in relation to the group that received the probiotic and their respective controls. There was also lower expression of the pro-inflammatory cytokine IL-6 in the PD + RACR group compared to the other groups. The use of Lactobacillus rhamnosus EM1107 in the adjuvant treatment of experimental ligature-induced periodontitis in mice was able to modulate the oxidative stress generated from the reduction in the level of malondialdehyde (MDA) and although there was no statistically significant difference, it was able to reduce the inflammatory marker IL-1β.
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6
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DÉBORA FROTA COLARES
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IMMUNOEXPRESSION OF E-CADHERIN , SNALIL 1 AND VIMENTIN PROTEINS IN SALIVARY GLAND TUMORS
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Advisor : LELIA BATISTA DE SOUZA
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COMMITTEE MEMBERS :
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CIRO DANTAS SOARES
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LELIA BATISTA DE SOUZA
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MARCIA CRISTINA DA COSTA MIGUEL
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Data: Aug 3, 2022
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Show Abstract
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Salivary gland tumors (SGTs) comprise about 2% to 10% of head and neck tumors and are known for their morphologic diversity and biologic behavior. Some features of malignant neoplasms, such as tumor invasion and distant metastasis, might have the participation of epithelial-mesenchymal transition (EMT), event in which proteins like E-cadherin, Vimentin and Snail1 are directly involved. This study aimed to analyze, by means of immunohistochemistry, the expression of these proteins, as well as to relate their expressions to clinical-pathological features of pleomorphic adenomas (PAs), adenoid cystic carcinomas (ACCs) and carcinomas ex-pleomorphic adenomas (CXPAs) located in minor and major salivary glands. This was a semi-quantitatively analysis which comprised 20 PAs, 20 ACCs and 10 CXPAs. Analysis of E-cadherin was made considering membranar and/or cytoplasmatic expression in parenchymal cells. For Snail1, it was considered the reaction of the protein in nucleus and/or cytoplasm of parenchymal cells. Vimentin was evaluated in cytoplasm of fusiform stromal cells. Data were compared and correlated adopting a level of significance of 5% (p ≤ 0,05). Marked immunoexpression for E-cadherin was found mostly in the cytoplasm of non-luminal neoplastic cells in SGTs; membrane reaction for the protein, seen in luminal cells, was higher in malignant tumors (p = 0,041). Snail1 was more expressed in nucleus, mostly of non-luminal cells of SGTs, with this reactivity being higher in malignant tumors (p = 0,012). Nuclear positivity for this marker was also higher for ACCs and CXPAs when compared with PAs separately (p = 0,037 e p = 0,025, respectively). No significant differences between E-cadherin and Snail1 and other clinical-pathological parameters were found (p > 0,05). Vimentin was seen in the stroma of all cases, being more diffuse and intense in ACCs. No significant differences between this marker and clinical-pathological parameters were found (p > 0,05). Positive correlations between membranar and cytoplasmatic E-cadherin in PAs, ACCs and CXPAs were observed (p = 0,002; p < 0,001; p = 0,031), as well as between nuclear and cytoplasmatic Snail1 and between cytoplasmatic E-cadherin and nuclear Snail1 in PAs (p = 0,009; p =0,032). Negative correlations between membranar E-cadherin and cytoplasmatic Snail1 were observed, as well as between nuclear Snail1 and Vimentin in ACCs (p = 0,036; p = 0,021). This last correlation, as well as positive correlation between membranar and cytoplasmatic E-cadherin were also seen when ACCs and CXPAs were grouped (p = 0,0011; p < 0,001). These results suggest that the participation of these proteins in EMT might be related to cellular differentiation in PAs and to tumoral progression in malignant tumors. In addition, it can be infered that the expression of E-cadherin and Snail1 in malignant tumors might reflect the plasticity seen in EMT process. Furthermore, the possible rule of Vimentin in the identification of neoplastic cells, in later stages of EMT, in the stroma of SGTs, is highlighted.
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7
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RAFAELA ALCINDO SILVA DE SOUSA FÉ
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EVALUATION OF THE PHYSICAL, CHEMICAL AND OSTEOGENIC PROPERTIES OF PORTLAND CEMENT ASSOCIATED WITH NIOBIUM
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Advisor : AURIGENA ANTUNES DE ARAUJO
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COMMITTEE MEMBERS :
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DANIEL PINTO DE OLIVEIRA
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FABIO ROBERTO DAMETTO
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RODRIGO OTHAVIO DE ASSUNCAO E SOUZA
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Data: Aug 4, 2022
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Show Abstract
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Mineral Trioxide Aggregate (MTA) is a material used for reparative purposes in Endodontics, characterized by its biocompatibility, radiopacity, low solubility and antimicrobial action. Composition of 75% Portland Cement (CP), 20% Bismuth Oxide (Bi2O3) and 5% Gypsum (CaSO4). However, Bi2O3 compromises essential properties of MTA. This work aimed to develop, characterize and evaluate the osteogenic activity of PC formulations with different proportions of Niobium oxide (Nb2O5), replacing Bi2O3. Three groups were used, one control and two experimental: GC (MTA Angelus®), F6 (75% CP, 20% Nb2O5 and 5% CaSO4) and F7 (75% CP, 10% Bi2O3, 10% Nb2O5e 5% CaSO4) . The formulations were submitted to X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR), X-ray Fluorescence Spectrometry (FRX), pH, setting, radiopacity, compressive strength, cytotoxicity and bioactivity. The data obtained were statistically evaluated by the test of variance (ANOVA) with Bonferroni correction (p<0.05%). The pH test result: Niobium 10% Immediate (12.205 ±0.304); 24h; (12.770 ± 0.226) 48h: (12.910 ± 0.169). Niobium 20%: immediate (12.080 ± 0.282); 24h: (12.350 ± 0.593); 48 h: (12.580 ± 0.73). For initial setting time in seconds: MTA (397,500 ±10,606); Niobium 10% (294,333 + 90,897) and Niobium 20% (279,000 + 15,874). The final setting time for the groups were: MTA (15,000 + 49,497), Niobium 10% (560 ± 38,587), being smaller when compared to MTA (p<0,001) and Niobium 20% (715,666 ± 30,664) (p<0 .01). Radiopacity values in mm Al of 10% Niobium (3.888 ±0.340); Niobium 20% (3.713 ± 0.712). The compressive strength in MPa was: Niobium 10% (694.150 + 78.951) Niobium 20% (699.295 + 47.672). Cell viability showed no difference between MTA and experimental groups (p<0.05). The results of the osteogenesis capacity of the formulations from the alkaline phosphatase assay (ALF) in IU/L per gram of protein for each group were: MTA (1.9 + 1.227) and Niobium 20% (1.784 + 1.342) (p> 0.05). Our findings point to relevant properties for Nb2O5 formulations such as alkaline pH, radiopacity, compressive strength and alkaline phosphatase activity. We suggest additional in vivo studies to prove biocompatibility.
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8
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CAIO RODRIGUES MAIA
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Use of Cell Morphometry in the Neoplastic Microenvironment and Its Relation to Interleukin-4 Immunoexpression.
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Advisor : PEDRO PAULO DE ANDRADE SANTOS
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COMMITTEE MEMBERS :
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PEDRO PAULO DE ANDRADE SANTOS
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CARLOS AUGUSTO GALVAO BARBOZA
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KEILA MARTHA AMORIM BARROSO
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Data: Aug 8, 2022
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Show Abstract
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Keratinized stratified squamous epithelium is a type of lining epithelial tissue that is present in the skin and mucous membranes that are predominantly made up of keratinocytes. These cell types produce keratin and have a polyhedral morphology with ovoid nuclei, but they can modify their phenotype according to the degree of differentiation in the layers of the epithelial component. The normal morphology of keratinocytes may vary according to the physiological events that occur, such as changes in the regularity and size of the nucleus at different stages of apoptotic, mitotic or replicative activity. However, cellular alterations may be present in some malignant neoplasms, such as squamous cell carcinoma and basal cell carcinoma, modifying the original morphology of keratinocytes as a result of an eventual alteration of the malignant phenotype of these cells. Interleukin-4 (IL-4) is a cytokine secreted by several types of cells, being involved in the development and differentiation of Th2 cells, acting in the anti-inflammatory response, its expression also seems to be related to the development of some neoplasms, including oral cancer. The objective of the present research will be to perform a cellular morphometric analysis (nuclear and cytoplasmic compartments), identification of nuclear irregularities of malignant keratinocytes and compare with the immunohistochemical expression of IL-4 in cases of Epidermoid Carcinoma of the Lower Lip (n:30) and Oral Tongue (n:30) using biotechnology tools for this purpose, contributing to the understanding of the biological behavior of these cell types in malignant neoplasms.
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9
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MÍRIA RAFAELLI SOUZA CURINGA
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DIGITAL SURVEYNG OF PARTIAL EDENTULOS FOR REMOVABLE PARTIAL DENTURE PLANNING: ACCURACY STUDY
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Advisor : ADRIANA DA FONTE PORTO CARREIRO
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COMMITTEE MEMBERS :
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ADRIANA DA FONTE PORTO CARREIRO
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ANA CLÁUDIA PAVARINA
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RODRIGO OTHAVIO DE ASSUNCAO E SOUZA
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Data: Aug 8, 2022
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Show Abstract
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This diagnostic study aimed to analyze a diagnosis of digital design in relation to the conventional of edentulous implanted archwires for planning removable partial dentures (RPD). A was composed of 143 dental faces in 30 sampled arch models presented from conventional models (Tesselation Standard Language), which methods were selected: delineation of conventional models (Group DC), from the impression of conventional and digital models (Group DV ), with the model STL files imported into Dental Wings software (specified manufacturer, country). A specific form was designed to collect the following questions: guide plans, retentive areas, reciprocity and design time. It was accurate and local accuracy between questions and the number of arc propiazation, between the conventional line and the digital one. The result showed low accuracy for the guide plane factor (0.57) and measured for retentive areas (0.67) and reciprocity (0.72). The time required to perform the digital design was shorter (2.87 ±0.95 minutes) than that required for the conventional method (3.95 ±1.61 minutes) (<0.001). The coincidence between the methods was not influenced by the location of the arc. However, the greater the amount of prosthetic spaces, the smaller the coincidence between the methods for the guiding plane and retentive area factors. Conclusion: The digital method of design showed low to medium accuracy compared to the conventional method. However, a visualization of retentive areas by a gradient of nuclei facilitates the identification of the determining factors and more objective for the faster digital technique for the dichotomy of the need to prepare the pillar elements in PPR.
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10
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FERNANDA GURGEL DE GOIS MOREIRA
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INFLUENCE OF PRINT TYPE, POST PROCESSING AND RESIN TYPE ON FLEXURAL STRENGTH AND DIMENSIONAL STABILITY OF PRINTED RESINS.
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Advisor : RODRIGO OTHAVIO DE ASSUNCAO E SOUZA
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COMMITTEE MEMBERS :
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RODRIGO OTHAVIO DE ASSUNCAO E SOUZA
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SAMIRA ALBUQUERQUE DE SOUSA
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RENATA MARQUES DE MELO
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Data: Aug 9, 2022
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Show Abstract
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Objective: To evaluate the influence of different post-processing protocols and the type of 3D printing of different printed resins on the three-point bending strength (s) and dimensional stability. Methodology: A bar was modeled (26 x 2.2 x 2.2mm) on Mishmixer software and a STL file was obtained, the it was exported to the 3D printer software SLA (Forms 2/Formslab) and LCD (FlashForge Photo 6.0/ FlashForge). Then, 45 bars of each type of resin (Pr - Provisional/Cosmos Temp, Yller; Pl - Occlusal Plate/Cosmos Splint, Yller; Mo – Models/ Cosmos Model, Yller) were printed by each printing system (SLA and LCD). After printing, the bars of each type of resin were cleaned with isopropyl alcohol and randomly divided (n=15) according to factors: “Post-processing protocol” Control (C: without post-processing); Ultraviolet Light (UV: 15 min in Anycubic Wash and Cure Plus, Anycubic) and Microwave (M: 15 min in microwave oven/1,400W). Thein, the bars were submitted to the s test (100KgF, 1mm/min) and data (MPa) analyzed with 2-way ANOVA and Tukey (5%). Complementary analyzes of dimensional stability (analyzed by paired T tests and 2-way ANOVA) and SEM of surfaces and fractures were also performed. Results: For flexural strength, ANOVA revealed that the factors "Printer" (p<0.05) and "Post processing" (p<0.05) and the interaction between them (p<0.05) presented statistical significance for all the types of resin used in this study. In turn, the groups processed in ultraviolet light, whether in model resin: SLAMod-UV (33.80±4.82AMPa), LCDMod-UV (35.93±4.47AMPa); provisional: LCDProv-UV (52.06±12.40AMPa), SLAProv-UV (42.86±2.09BMPa) or oclusal split: LCDPl-UV (91.46±12.33AMPa), LCDPl-UV (33.20 ±4.84BMPa) showed higher values of , being statistically superior to the groups without microwave processing and post-processed (Tukey 5%).Conclusion: Post-processing in ultraviolet light should be the choice regardless of type of resin, since this protocol promotes better values of flexural strength and that this strength varies according to the type of resin. As well, that the LCD printing technique for the three types of resin can be the most indicated when associated with post processing in ultraviolet light.
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11
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MARCELLA GUEDES PEREIRA GOUVÊA BEZERRA
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INFLUENCE OF THE BUILD ANGLE AND THICKNESS OF THE PRINTING LAYER ON THE FLEXURAL STRENGTH, DIMENSIONAL ACCURACY AND ROUGHNESS OF PRINTED RESIN
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Advisor : RODRIGO OTHAVIO DE ASSUNCAO E SOUZA
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COMMITTEE MEMBERS :
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RODRIGO OTHAVIO DE ASSUNCAO E SOUZA
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LAERCIO ALMEIDA DE MELO
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ANDRE ULISSES DANTAS BATISTA
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Data: Aug 23, 2022
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Show Abstract
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Objective: To evaluate the influence of printing layer angulation and thickness on three-point flexural strength (s), dimensional stability and roughness of printed provisional resin. Methodology: From the modeling of a bar (26 x 2.2 x 2.2mm) in Meshmixer software (Autodesk) and obtaining an STL file, it was exported to the SLA 3D printer software (Forms2/Formlabs), where 225 bars of the same dimensions were printed with Cosmos Temp resin, Yller (n=15) according to the factors “printing layer thickness” (25μm;50μm;100μm) and “angulation”(00; 300; 450; 600 and 900). After printing, the samples were cleaned with isopropyl alcohol and sent to post-processing in an ultraviolet oven (Anycubic Wash and Cure Plus, Anycubic) for 15 minutes. The bars were submitted to the s test in a universal testing machine (100KgF,1mm/min) and the data (MPa) analyzed with 2-way ANOVA, Tukey (5%) and Weibull analysis. Roughness and dimensional stability analyzes were also performed (data analyzed by 2-way ANOVA and Tukey test (5%) and surface SEM. Results: For flexural strength, 2-way ANOVA revealed that the factor “Print layer thickness” (p<0.0001) was statistically significant, while the factor “Angulation” (p=0.8074) was not statistically significant. The 25μm thickness showed the highest values of s (47.29AMPa). 25μm (51.2±4.6AMPa), 60°/25μm (49.1±4.3ABMPa), 0°/25μm (48.6±6.6ABMPa), 90°/50μm (46.8±4.1ABCMPa) and 90°/25μm (46.2±4.9ABCMPa) showed s values statistically higher than the 0°/100μm (39.4±5.4DEFMPa), 60°/100μm (37.7±4.2DEFMPa), 90°/100μm (37.1±4.1EFMPa) and 30°/100μm (34.8±4.5FMPa), which were similar to each other. For dimensional stability, the thickness 25μm (1100.3Aμm) and angulation 0° (11571Aμm) showed greater dimensional changes. The groups 0°/50μm (1746.9±61.80Aμm) and 0°/100μm (1704.7±84.30Aμm) showed the greatest dimensional changes, while the 90°/25μm group (401.1±48.61Gμm) showed the smallest changes. For surface roughness, the thickness of 100μm (0.6452A μm) and the angulations 45° (0.6717Aμm), 30° (0.6193Aμm) and 60° (0.6127Aμm) presented higher values. The 30°/100μm (0.90±0.10Aμm), 45°/100μm (0.79±0.07ABμm) and 60°/100μm (0.88±0.08Aμm) groups were similar and presented the highest values, while the 90°/ 100μm (0.23±0.08F) showed the lowest values. Conclusion: The lower the thickness, the greater the flexural strength, regardless of the chosen angle. For dimensional stability, the smaller the thickness of the printing layer, the greater the dimensional variation, while objects printed vertically (90°) have greater dimensional stability. The greater the thickness, the greater the surface roughness. The 30°, 45° and 60° angles have rougher surfaces, while the 90° angles are smoother.
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12
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EMMILY TAMIRIS FARIAS PINTO DE MACEDO
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Assessment of knowledge and clinical management of orthodontists regarding Molar Incisor Hypomineralization
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Advisor : SERGEI GODEIRO FERNANDES RABELO CALDAS
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COMMITTEE MEMBERS :
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SERGEI GODEIRO FERNANDES RABELO CALDAS
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ISABELITA DUARTE AZEVEDO
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ARIANE SALGADO GONZAGA
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Data: Aug 25, 2022
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Show Abstract
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Introduction: Molar Incisor Hypomineralization (MIH) is a qualitative defect of dental enamel, which makes it fragile and porous, and can interfere with orthodontic practice. Thus, it is necessary for the orthodontist to know this enamel change. Aim: To assess the level of knowledge and clinical practices of orthodontists in relation to MIH. Methodology: We performed a cross-sectional observational study among brazilian orthodontists to evaluate their practices and previous knowledge regarding MIH. We developed a questionnaire including questions about MIH diagnosis, clinical experience and orthodontic approaches regarding MIH. The questionnaire was validated by pediatric dentists (using the Delphi method) and sent to orthodontists acting in all brazilian regions. Orthodontists who did not reside in Brazil and those who were temporarily unable to practice the speciality were excluded from this study. At total, responses from 351 orthodontists were considered. Results: Although 80.1% orthodontists claimed to know the HMI, 44.2% said they did not feel confident in carrying out their diagnosis. There was no significant association between the level of knowledge of these professionals and the sociodemographic variables (p>0.05), with the exception of the gender variable, where females showed satisfactory knowledge regarding MIH, when compared to males (p<0. 05). The HMI has been noticed by 74.4% of the participants in their clinical routine, and 80.3% stated that these changes directly affect their orthodontic practices. Conclusion: Although orthodontists know the MIH, they do not fully master its characteristics and peculiarities. There are no ideal protocols in the literature for the conduct of bonding, cementing and debonding of orthodontic accessories in teeth with MIH, which highlights the need for further studies in this regard. In this way, the orthodontist will be able to help in the preservation of teeth with MIH, avoiding possible iatrogenesis during the orthodontic treatment and conducting the case in the best possible way.
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13
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WANDERSON TALLES DO NASCIMENTO PEREIRA SANTOS
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Influence of tooth whitening on the surface hardness of three composite resins: An In Vitro Study
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Advisor : ISAUREMI VIEIRA DE ASSUNCAO
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COMMITTEE MEMBERS :
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AMANDA MARIA FERREIRA BARBOSA
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ISAUREMI VIEIRA DE ASSUNCAO
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PRISCYLLA GONCALVES CORREIA LEITE DE MARCELOS
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Data: Aug 26, 2022
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Show Abstract
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This study aims to evaluate in vitro the surface hardness of three polymerizable composite resins (Filtek Z350 - 3M ESPE, Zirconfil - TECHNEW, Vitra - FGM), after a 40% hydrogen peroxide tooth bleaching process. 60 specimens were made, 20 specimens of each restorative material (n=20). After the finishing and polishing process, the specimens were randomly divided into two groups for each restorative material (n=10), where the control group G1 was maintained in artificial saliva and the test group G2 was subjected to bleaching gel based on of 40% hydrogen peroxide according to the manufacturer's instructions. For each specimen, four consecutive and equidistant readings were performed, with a load of 300g of force for 15s. Hardness values for tested resins were correspondingly higher in the control group than in the test group. The biggest difference in the mean values between the groups occurred in the Z350XT resin and for the fixed margin of error (5%) a significant difference (p < 0.05) between the test and control groups for each resin. The hypothesis tested in this test was null, since there were significant changes between the hardness of the control group and the test group, thus showing that the bleaching gel altered the hardness of the tested resins.
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14
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TATIANA BERNARDO FARIAS PEREIRA
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RISK FACTORS ASSOCIATED TO THE DEVELOPMENT OF ORAL alterations IN PATIENTS UNDER HEMATOPOIETIC STEM CELL TRANSPLANTATION: A COHORT STUDY
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Advisor : ERICKA JANINE DANTAS DA SILVEIRA
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COMMITTEE MEMBERS :
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ERICKA JANINE DANTAS DA SILVEIRA
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ANA RAFAELA LUZ DE AQUINO MARTINS
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JAMILE MARINHO BEZERRA DE OLIVEIRA MOURA
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Data: Aug 30, 2022
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Show Abstract
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Hematopoietic stem cell transplantation can cause adverse effects on the oral environment that can affect the patient's response to treatment and the prognosis. Thereby, this study aimed to identify the most prevalent oral alterations during the period of hospitalization for the performance of HSCT, to analyze the development time of these alterations in this period, as well as the factors associated with the development of these alterations. It was an observational longitudinal, cohort study carried out in patients that was assisted by the bone marrow transplant sector in a main hospital in Rio Grande do Norte between December 2021 and June 2022. Data about oral physical examination, diagnosis of hematological disorder, type of transplant, comorbidities, chemotherapy protocols and local risk factors of patients obtained during the HSCT period were submitted to the Kaplan-Meyer test, parametric logrank test and univariate COX regression to estimate the time of oral changes' onset of and association of risk factors. The statistical significance adopted was 5%. 57.7% of the 30 selected patients were male, median age of 35 years, where 93.3% developed some oral alteration and 53.3% started treatment with some risk factor. The most frequent neoplasms were leukemias (myeloid and lymphoid) and multiple myeloma (23.3% each). FluBuMel was the most used conditioning protocol (40%). The most frequent oral alteration was buccal mucosa edema (83.3%) followed by oral mucositis (80%; grade 1 - 54.2%). The follow-up time was on average 23 days and it was found that on the 5th day of hospitalization the probability of the patient being free of alterations is 93.3%, with this rate decreasing over time, reaching 6.7% in the 28th day. The Mel200 protocol is associated with earlier development of alterations (p=0.02), as well as autologous transplantation (p=0.004). The results suggest that the type of transplant and the chemotherapy conditioning protocol affects the time of onset of oral alterations and additionally reinforce that the low severity of the oral alterations is directly related with the presence of a dentist in the multidisciplinary team.
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15
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FELIPE ALEXANDER CALDAS AFONSO
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COMPARATIVE STUDY OF THE HEAD ORIENTATION METHODS FOR 3D VIRTUAL PLANNING OF ORTHOGNATHIC SURGERIES
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Advisor : ADRIANO ROCHA GERMANO
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COMMITTEE MEMBERS :
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ADRIANO ROCHA GERMANO
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JOSE SANDRO PEREIRA DA SILVA
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LIOGI IWAKI FILHO
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Data: Sep 14, 2022
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Show Abstract
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INTRODUCTION 3D virtual computer simulation was a paradigm shift in the treatment of dentofacial deformities, however, the best way to achieve the Neutral Head Position (NHP) is still controversial. There is a lack of comparative studies between the present protocols to assess the degree of precision of the methods. This work aims to evaluate the degree of discrepancy between NHP methods in a virtual environment. METHODS Ten consecutively selected patients with dentofacial deformities were included in the analysis. Each patient underwent all the following protocols for acquiring the neutral head position: (A) The Procrustes Method – Primary Sagittal Plane; (B) The Charlotte Protocol Method; (C) The Universal Protocol Method. Cone-beam computed tomography (CBCT) with CR bite registration were requested and impressions of both dental arches were taken for 3D scanning. Then, for the preparation and positioning of the composite skulls in the NHP, the Anatomic Aligner software (Houston Methodist Research Institute) was used. The discrepancies were evaluated by superimposing the 3D craniofacial models using the Gateno-Xia cephalometric analysis, followed by measurements of linear and angular differences. RESULTS In the cases analyzed in this study, when comparing the NHP acquisition methods with each other, the linear and angular values obtained are within of the limits recommended in the literature as being excellent (angular differences less than 2mm and 4°), with the largest linear deviation of 1.56 mm, and orientation of 1.96°, considering all oriented composite skulls. In addition, paired comparative analyzes between the NHP methods were performed, following those comparisons: Group A against Group B; Group A against Group C; and Group B against Group C. The authors observed that there were slight deviations from the values recommended in the literature only when comparing the anteroposterior linear axes Y (mm) between groups A and B, with 2.64 mm, and between groups B and C, with 2.2mm. Finally, statistical analysis of ANOVA did not show any statistically significant difference. DISCUSSION The authors confirm the hypothesis that all head orientation methods evaluated in this study do not present statistically significant differences (P>0.05) and consider that when and orientation of the skull-composite in a controlled environment and by professionals specialized and trained in the surgical area, all techniques for orientation of the head can be useful, accurate and valid for the desired result. This is because all this preparation is based on facial analysis and clinical data collected by the surgeon. CONCLUSION This study showed that there was no statistical difference in terms of precision between all the protocols listed for comparison, and it proved to be extremely important to provide surgeons with a reflection on which technique would be most appropriate for the treatment of patients with dentofacial deformities in its center, always considering the main aspects that facilitate the surgeon's clinical day: Simplicity of execution; less time spent; and familiarity with the technique.
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16
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ANNA CRISLLAINY DA COSTA MONTEIRO
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OCCURRENCE AND SEVERITY OF MOLAR-INCISIVE HYPOMINERALIZATION IN CHILDREN AND ADOLESCENTS: A serial panel study
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Advisor : KENIO COSTA DE LIMA
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COMMITTEE MEMBERS :
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KENIO COSTA DE LIMA
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RENATA SARAIVA GUEDES
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MARINA DE DEUS MOURA DE LIMA
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Data: Sep 16, 2022
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Show Abstract
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Introduction: Molar-Incisor Hypomineralization (MIH) is an enamel development defect of systemic origin that affects at least one permanent first molar and may be associated with permanent incisors. Clinically, it is characterized as demarcated enamel opacities that can vary in color (white-cream or yellow-brown). Objective: To estimate the occurrence and severity of MIH in children and adolescents and identify possible factors associated with the occurrence of MIH. Methodology: A cross-sectional study was carried out in serial panels (2002 to 2011) and (2012 to 2021) using 230 medical records of patients aged between 6 and 12 years, undergoing orthodontic treatment at the Brazilian Dental Association between 2002 and 2021. The diagnostic criteria used were the presence or absence of demarcated opacities, atypical restorations, post-rupturing fractures and molar extraction due to HMI. The collected data were typed and processed in the SPSS program, statistically treated using the chi-square test at a confidence level of 95%. Results: The occurrence of MIH throughout the study period was 26 cases among the 230 medical records evaluated (which represents 11.3% with a 95% CI from 7.21 to 15.39%). Of these, 03 cases are severe, which is equivalent to 11.5% (95% CI). An association of the occurrence of MIH with the variables gender (male), age (06 to 10 years) and year in which the documentation was carried out (2012 to 2021) was observed. Conclusion:This work shows that although the occurrence of MIH is low in children and adolescents from 6 to 12 years old, it has been increasing in recent years. The percentage of male and younger individuals with HMI is higher, indicating an increasing trend in the occurrence of HMI in the coming years. In addition, most cases are mild, with greater involvement of molar teeth compared to incisors and maxillary molars and incisors compared to mandibular ones. These findings are important to help formulate strategies to prevent or reduce the consequences of HMI.
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17
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DANIELLE MACHADO FARIAS
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Imunoexpression of ING4, VEGF e NF-κB in inflamatory periapical lesions
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Advisor : LELIA MARIA GUEDES QUEIROZ
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COMMITTEE MEMBERS :
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EMANUEL SÁVIO DE SOUZA ANDRADE
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ERICKA JANINE DANTAS DA SILVEIRA
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LELIA MARIA GUEDES QUEIROZ
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Data: Sep 19, 2022
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Show Abstract
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IPLs are pathological conditions resulting from infections of odontogenic origin, being mainly represented by PGs and RCs. Its pathogenesis is associated with immunological and angiogenic mechanisms, characterized by the self-limitation of the inflammatory reaction. Therefore, this retrospective, semi-quantitative and comparative study aimed to analyze the immunohistochemical expression of ING4, VEGF and NF-κB in IPLs, and to correlate the pattern of expression of these proteins. The sample consisted of 62 IPLs, of which 41.9% were PGs, 27.4% RCs, 30.6% RRCs. Morphological analysis was performed, evaluating epithelial thickness and inflammatory infiltrate, and the correlation of these findings with the immunohistochemical expression pattern of ING4, VEGF and NF-κB proteins in IPLs. To perform the statistical analysis, the chi-square, Kruskal-Wallis, Mann-Whitney and Spearmen correlation tests were used. The intensity of the inflammatory infiltrate showed a significant association in the IPLs, showing greater intensity in the PG when compared to the cysts. Furthermore, the RCs showed a higher intensity of the inflammatory infiltrate when compared to the RRCs. By associating the expression of ING4 in inflammatory cells of the connective tissue or fibrous capsule, the PGs and RCs showed greater expression of this protein both at the nuclear and cytoplasmic levels. Immunoexpression of VEGF in the nucleus of inflammatory cells of the connective tissue or fibrous capsule shows a significant association with IPLs, with greater expression of this protein in the cysts than in the PG. There was a statistically significant association between NF-κB immunostaining and IPLs, in which PGs exhibited higher protein expression in relation to RCs and RRCs. When compared between the cysts, there was no statistically significant association, however the median of NF-κB expression was higher for the RCs. In the fibrous capsule, nuclear immunoexpression of NF-κB in inflammatory cells was higher in periapical lesions with intense inflammatory infiltrate. Similarly, the cytoplasmic immunoexpression of NF-κB in inflammatory cells was higher in cases of periapical lesions that presented intense inflammatory infiltrate. Therefore, it is suggested that ING4, VEGF and NF-κB participate in the etiopathogenesis of IPLs, and that there is a directly proportional relationship between the expression of these proteins.
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18
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HEVILA DE FIGUEIREDO PIRES
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AMELOBLASTOMA RECURRENCE: PROGNOSIS STUDY
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Advisor : HEBEL CAVALCANTI GALVAO
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COMMITTEE MEMBERS :
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HEBEL CAVALCANTI GALVAO
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ROSEANA DE ALMEIDA FREITAS
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MANUEL ANTONIO GORDON NUNEZ
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Data: Sep 23, 2022
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Show Abstract
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Introduction: Ameloblastoma (AMB) is a benign odontogenic neoplasm that has high rates of postoperative recurrence. Several studies show the relationship between clinical-pathological characteristics and treatment modalities in AMB recurrence. The molecular mechanisms involved in the etiopathogenesis of this tumor are poorly understood, and although alterations in the Mismatch System (MMR) favor the development of different human neoplasms, their importance in the development of ameloblastoma remains poorly understood. Objective: To identify the prognostic factors associated with ameloblastoma recurrence, as well as to investigate the role of the immunoexpression of hMLH1, hMSH2 and KI-67 proteins in the recurrence of these odontogenic tumors. Methodology: This was a descriptive, cross-sectional and retrospective study, with a sample consisting of 22 cases of recurrent ameloblastomas and 22 non-recurring cases. The immunohistochemical analysis was performed quantitatively, considering the cellular (nuclear) location of the proteins studied. McNemar's test was used to compare the variables between primary and recurrent ameloblastoma lesions. Recurrence-free survival was analyzed using the Kaplan-Meier method and survival functions were compared according to variables using the log-rank test. Results: The total sample was mostly female (n=24; 54.5%), with a mean age of involvement of 39.1 ± 19.8 years, and 45.5% (n=20) were caucasian. The posterior mandible region was the most frequent in the relapsed group (n=18, 81.8%) and also for the cases that did not present relapses (n=16, 72.8%). The recurrence-free time was 50.0 (34.5 – 63.6) months. Factors significantly associated with the recurrence of AMBs were: expansion of cortical bone (p=0.0089), presence of bone reconstruction (p=0.018), conservative treatment (p=0.021), loss of hMSH2 immunoexpression (p=0.006) and hMLH1 (p=0.038) and strong immunoexpression of KI-67 (p=0.029). Conclusion: Based on the findings of this research, it is concluded that radiographic appearance, treatment modality and immunoexpression of proteins from the Mismatch System and KI-67 are the most significant prognostic factors for the recurrence of AMBs.
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19
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NATÁLIA CRISTINA GARCIA PINHEIRO
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KNOWLEDGE, NEED AND PREFERENCE ABOUT HOME DENTISTRY AND ORAL HEALTH CONDITIONS OF DOMICILED ELDERLY: a cross-sectional study
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Advisor : KENIO COSTA DE LIMA
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COMMITTEE MEMBERS :
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KENIO COSTA DE LIMA
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ANA RAFAELA LUZ DE AQUINO MARTINS
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GUSTAVO PINA GODOY
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Data: Sep 23, 2022
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Show Abstract
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Objective: The study aims to understand the knowledge, preference and need for dental care (AOD), especially for the elderly population, in addition to identifying the oral health condition of the elderly living and its associated factors. Methodology: These are two web surveys, the first with the educated adult population and the second with the elderly population. In addition, a study was also carried out with secondary data from medical records of elderly people who used some home dental care service to identify their oral health condition. In the three studies, descriptive analysis, chi-square test and robust Poisson regression were performed with a significance level of 5%. elderly in relation to dental and rehabilitation status. Results: In the first study, 443 respondents participated, whose predominance was women (67.9%) and aged between 31 and 40 years (38.8%). Most participants (69.5%) do not know what home dental care is. The fact of being a woman and a health professional directly influences the knowledge of this type of care. In the second study, 203 elderly people participated, 67% of whom were women, aged between 60 and 69 years. The majority of this population is not familiar with home dentistry (79.8%), has never used this type of service (95.6%) and prefers to be seen in a dental office (68.5%). There was a significant association between knowing about home dentistry and having a medical health plan. It was also observed that those elderly who do not have health insurance and with a lower level of education prefer to be cared for at home in relation to the traditional office. In the third study, 207 medical records of the elderly were evaluated, whose predominant sex was female (58.5%), aged between 80 and 89 years and partially dependent for basic activities of daily living. Regarding oral health status, the mean DMFT was 23.5, with 3.35 pairs in occlusion, 75.8% of whom were functional edentulous. In the medical records, 53.6% of the elderly had bleeding on probing in some sextant and about 20% had a mouth lesion. Patients with more injuries were younger elderly, diabetics and those who had been to the dentist for a longer period of time. Regarding the periodontal issue, younger patients, single residents and whites have more bleeding on probing. Regarding the dental and rehabilitation condition, two clusters were produced, the first with edentulous and poorly rehabilitated elderly and the second with more dentate and rehabilitated elderly. Black elderly people who do not have health insurance were more present in the first cluster. Conclusion: The general and elderly population knows little and realizes that they need home dental care. As a result, the more independent elderly population still prefers to be seen in the office. Furthermore, the oral health condition of patients who use home dental care is still precarious, as a result of mutilating dental care, characterizing the scenario of social inequality among elderly people living in domicile.
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20
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NILMÁRIO OLIVEIRA DE SOUSA
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SURVIVAL OF CERAMIC RESTORATIONS AND THEIR ASSOCIATED FACTORS
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Advisor : ERIKA OLIVEIRA DE ALMEIDA FREITAS
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COMMITTEE MEMBERS :
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AMILCAR CHAGAS FREITAS JÚNIOR
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ERIKA OLIVEIRA DE ALMEIDA FREITAS
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FERNANDO JOSE DE OLIVEIRA NOBREGA
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Data: Sep 27, 2022
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Show Abstract
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Ceramic restorations can be essential in aesthetic and functional dental rehabilitation, but their longevity and treatment plan need to be well established. Different material options, fabrication techniques and protocols are available, but there is little scientific literature explaining the factors that generate the main failures and clinical complications related to these fixed prostheses. Thus, the present study aimed to evaluate the survival of ceramic restorations and their associated factors. Therefore, an observational, retrospective and longitudinal cohort research was carried out to follow up different types of ceramic restorations installed in the last 10 years and made following the same clinical protocol. A total of 32 patients were evaluated, totaling 256 fixed prostheses, regarding the presence of failures or complications, type of ceramic system, whether the patients had a diagnosis of sleep bruxism and whether they used an occlusal splint and the type of cement used for cementation. Participants attended a clinical evaluation to analyze the integrity of the prosthesis, answered questions that address signs and symptoms of bruxism, such as tooth wear, muscle hypertrophy and indentations. Additional information about time and materials used to make the restorations was collected from the patients' medical records. The data obtained were analyzed using the SPSS program (Statistical Package for the Social Science) 20.0 with the Chi-Square or Fisher's Exact test, with a confidence level of 95%. The results showed success and survival rates of 84.37% and 96.1%, respectively. Considering the correlation with prosthetic failures, the male gender (p=0.017), the presence of sleep bruxism (p=0.004) and wakeful bruxism (p=0.009) had a positive influence. As for the interaction with prosthetic complications, the presence of sleep bruxism (p=0.040), the type of prosthetic piece (p=0.014) and the type of cement (p=0.043) were statistically significant. Within the limitations of the study, it can be concluded that ceramic restorations had high success and survival rates, ensuring the longevity of this type of rehabilitation. Sleep bruxism and awake bruxism are risk factors for ceramic restorations.
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21
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NIZYARA COSTA DA SILVA
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JAW OSTEOMYELITIS: CLINICAL PATHOLOGICAL STUDY
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Advisor : ANTONIO DE LISBOA LOPES COSTA
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COMMITTEE MEMBERS :
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ANTONIO DE LISBOA LOPES COSTA
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HEBEL CAVALCANTI GALVAO
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MARIA DE LOURDES SILVA DE ARRUDA MORAIS
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Data: Sep 30, 2022
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Show Abstract
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Osteomyelitis is defined as an inflammatory state of the bone and its etiopathogenesis is multifactorial, including trauma, systemic diseases and odontogenic infections. Therefore, this research is an analysis study of the analysis of retrospective and comparative evaluation studies of the types of osteomyelitis and their evaluations, which consist of clinical-pathological characteristics such as clinical-pathological characteristics in the jaws, diagnosed in the Oral Pathology, Department of Dentistry of the UFRN lesion, in the period from January 1970 to December 2021. The sample consists of 75 cases of acute osteomyelitis, with 9 cases of acute osteomyelitis, 3 of primary chronic osteomyelitis and 63 cases of secondary chronic osteomyelitis. A collection of clinical data was carried out, from which demographic data, drinking and smoking habits, disability resources and treatments used were performed. Radiographic analysis of location considered bone loss, dimension, pat fracture, bone sequencing, reactional, and size. The morphological study evaluated osteoblasts, osteoclasts, bacterial colonies, necrotic bone and granulation tissue. For statistical analysis, the fisher tests and the chi-square test were used. The results found were revealed with the predominant female sex (n=67; 89.3%). The average age was 42,68 years, with variations between 3 and 85 years old, patients self-reported as white were predominant (n=37; 49.3%). As for the types of osteomyelitis, secondary chronic osteomyelitis was predominant (n = 63; 84%), followed by acute osteomyelitis (n = 9, 12%) and primary chronic osteomyelitis (n = 3; 4%). As for the anatomical location, the mandible was predominant with (n=70, 93.3%). As for the causes, odontogenic infection was more prevalent with (n=41; 54.7%). The inflammatory process was present in all cases (n=75; 100%). As for the types of osteomyelitis, secondary chronic osteomyelitis was predominant (n = 63, 84%), followed by acute osteomyelitis (n = 9, 12%) and primary chronic osteomyelitis (n = 3, 4%). As for the anatomical location, the mandible was predominant with (n=70, 93.3%). As for the causes, odontogenic infection was more prevalent with (n=41; 54.7%). The inflammatory process was present in all cases (n=75; 100%). As for neutrophils, they were absent in most cases (n=51; 68%) and discreetly present in (n=18; 24%). In view of the results obtained, we conclude that osteomyelitis is more common in females with a mean age of 42.68 years, with the most common type being secondary chronic osteomyelitis, originating from odontogenic infection and located in the mandible. The association between the type of osteomyelitis and the presence or absence of bacterial colonies showed a statistically significant association. The same did not happen with the other statistical associations carried out in this research.
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22
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VLADIMIR GALDINO SABINO
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EFFECTS OF LOW-LEVEL LASER IRRADIATION ON OSTEOBLASTS CULTURED ON NANOFIBROUS POLYLACTIC ACID SCAFFOLDS
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Advisor : CARLOS AUGUSTO GALVAO BARBOZA
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COMMITTEE MEMBERS :
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CARLOS AUGUSTO GALVAO BARBOZA
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MARCIA CRISTINA DA COSTA MIGUEL
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CARLOS EDUARDO BEZERRA DE MOURA
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Data: Nov 28, 2022
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Show Abstract
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Bone tissue engineering is a relevant branch of regenerative medicine and involves the development of scaffolds with composition and architecture favorable to cell integration, in addition to studying factors capable of promoting cell adhesion and proliferation, including chemical and biophysical stimuli. The study aimed to evaluate the use of low-level laser irradiation (LLLI) to promote in vitro biostimulation of osteoblastic cells cultured on polylactic acid (PLA) nanofibrous scaffolds. The scaffolds were produced by the electrospinning technique and characterized in terms of wettability, composition by Fourier transform infrared spectroscopy (FTIR), surface morphology by scanning electron microscopy (SEM), thermogravimetric characterization (TGA), differential scanning calorimetry (DSC) and crystallinity by X-ray diffraction (XRD). The biological assays were conducted with osteoblasts of the OFCOL II lineage cultured on the surface of the scaffolds and submitted or not (control group) to irradiation with InGaAIP diode laser at doses of 1, 4 and 6 J/cm², power of 30 mW, at wavelengths of 660 nm (R, red light) and 780 nm (IR, infrared). After irradiation, the effects of LLLI on osteoblast proliferation were evaluated using the Alamar Blue biochemical method at intervals of 24, 48, and 72h. Cell viability and morphology were analyzed at 72h, using the Live/Dead assay and scanning electron microscopy (SEM), respectively. Data from the Alamar Blue biochemical assay showed more significant cell proliferation in groups R1 and R6, with R6 showing a significantly higher percentage of Alamar reduction compared to the control group at all intervals (p<0.05). Other differences between the control and irradiated groups were found only at intervals of 48h and 72h for R1 and 72h for IR6. The Live/Dead assay revealed an increase in cell viability in the laser-treated groups, significantly higher in the R1 group when compared to the control group. SEM analysis showed adequate interaction between osteoblasts and scaffolds in all groups, with the cell body spreading along the nanofiber axis, with more evident physical contacts being observed through the formation of bonds through filopodia and lamellipodia in groups R1, R6, and IR6, the same ones that exhibited proliferative highlights in the Alamar Blue assay. Taken together, the data from this study showed that LLLT promotes the biostimulation of osteoblasts cultured on PLA nanofibers, which points to its potential use in bone tissue engineering techniques.
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Thesis |
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1
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GLÓRIA MARIA DE FRANÇA
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The role of WNT/ß-Catenin, TGF-ß/BMP4 and SHH pathway proteins in odontogenesis and odontomas.
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Advisor : HEBEL CAVALCANTI GALVAO
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COMMITTEE MEMBERS :
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HEBEL CAVALCANTI GALVAO
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LEORIK PEREIRA DA SILVA
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MANUEL ANTONIO GORDON NUNEZ
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PEDRO PAULO DE ANDRADE SANTOS
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ROSEANA DE ALMEIDA FREITAS
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Data: Jan 25, 2022
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Show Abstract
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The Wingless (Wnt) / β-catenin signaling pathway is essential for the early activation of odontogenesis and the development of odontogenic tumors, additionally this pathway is responsible for the expression of high molecular weight cytokeratins, such as CK14 and CK19. TGF-β and BMPs have been associated with reactionary and repair dentinogenesis processes. This growth factor is released by odontoblasts and fibroblasts and is stored inside the dentinal tubules. Tooth development depends on a series of reciprocal signaling interactions between the oral epithelium (EO) and neural crest-derived ectomesenchyma, the WNT pathway with TGF-β and BMP4 has been implicated in the formation of supernumerary teeth during odontogenesis. Meanwhile, Shh signaling can regulate cell proliferation in the dental mesenchyme, thus controlling dental morphogenesis. Therefore, the objective of our work will be to investigate the role of these pathways in odontogenesis and in the formation of odontomas and benign mixed odontogenic tumors. For this, a retrospective and immunohistochemical sectional study was developed containing 23 compound odontomas, 21 complex odontomas, 17 dental germs, 05 ameloblastic fibro-odontomas and 01 ameloblastic fibroma. The results found showed greater immunoexpression of the WNT/ß-catenin pathway in the epithelium of tooth germs (p<0.001) and in ameloblastic fibroma, whereas it was in the ectomesenchyma of odontomas (p<0.001) and ameloblastic fibro-odontomas. WNT/ß-catenin correlated moderately and significantly with CK14 in the epithelium (p<0.001) and ectomesenchyma of all lesions (p=0.018). BMP4 was immunoexpressed, especially in the mesenchyme of complex odontomas (median = 33.7; p<0.001). Shh was more immunoexpressed in the epithelium of tooth germs (p<0.001) and in the ectomesenchyma of complex odontomas (p=0.029). Similarly, TGF-ß showed greater immunoexpression in the epithelium of tooth germs (p<0.001) and in the ectomesenchyma of complex odontomas (p = 0.002). Finally, Odontogenesis exhibits higher concentrations for these proteins in the odontogenic epithelium in the early stages and the change in expression in the ectomesenchyma occurs, mainly, in the histodifferentiation in the bell phase, predisposing to the formation of odontogenic tumors.
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2
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EVERTON FREITAS DE MORAIS
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BIOMARKERS OF EPITHELIO-MESENCHYMAL TRANSITION IN POTENTIALLY MALIGNANT DISORDERS AND ORAL TONGUE SQUAMOUS CELL CARCINOMA
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Advisor : ROSEANA DE ALMEIDA FREITAS
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COMMITTEE MEMBERS :
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CIRO DANTAS SOARES
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HEBEL CAVALCANTI GALVAO
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LELIA BATISTA DE SOUZA
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RICARDO DELLA COLLETA
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ROSEANA DE ALMEIDA FREITAS
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Data: Feb 24, 2022
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Show Abstract
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During oral carcinogenesis, malignant cells acquire an aggressive phenotype that results in increased individual motility and the ability to invade surrounding tissues. Therefore, malignant epithelial cells develop a regulatory and programmed process called epithelial-mesenchymal transition (EMT), which is crucial for the acquisition of this aggressive malignant phenotype. The aim of the present study was to investigate the role of immunohistochemical expression of EMT signaling proteins in oral epithelial dysplasia and oral tongue squamous cell carcinoma (OTSCC), evaluating their respective associations with clinical-pathological parameters of prognosis. For the immunohistochemical study, 47 cases of oral epithelial dysplasia and 41 cases diagnosed as (OTSCC) were selected, in which the immunoexpression of the proteins Twist1, Snail1, E-cadherin and N-cadherin were analyzed. Possible associations between the expression pattern of these proteins with the histopathological gradation of epithelial dysplasias and with the clinical-pathological aspects, recurrence and survival in OTSCC were investigated. Different labeling patterns were observed between the analyzed groups, with a significant loss of membrane E-cadherin expression in cases of OTSCC compared to cases of oral epithelial dysplasia (p = <0.0001). A worse overall survival was observed in cases with low membrane E-cadherin expression (HR = 0.27; p = 0.033) and high cytoplasmic Twist1 expression (HR = 3.19; p = 0.010). When analyzing the expression intensity parameter alone, an association was observed between the high intensity of cytoplasmic N-cadherin and overall survival (HR = 4.93; p=0.006). Our findings suggest that loss of E-cadherin expression and increased expression of N-cadherin and nuclear transcription factors Twist1 and Snail1 are associated with the development and progression of oral carcinogenesis. In isolation, loss of membrane expression of E-cadherin and increased cytoplasmic expression of Twist1 and N-cadherin were associated with poorer survival.
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3
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VICTOR DINIZ BORBOREMA DOS SANTOS
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ASSESSMENT OF THE VALIDITY, REPRODUCIBILITY AND ACCURACY OF DIGITAL MODELS OBTAINED FOR VIRTUAL SURGICAL SIMULATION IN ORTHOGNATHIC SURGERY
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Advisor : JOSE SANDRO PEREIRA DA SILVA
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COMMITTEE MEMBERS :
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HECIO HENRIQUE ARAUJO DE MORAIS
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JOSE SANDRO PEREIRA DA SILVA
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JOSÉ WILSON NOLETO JÚNIOR
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MARCELO AUGUSTO DE OLIVEIRA SALES
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WAGNER RANIER MACIEL DANTAS
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Data: Feb 25, 2022
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Show Abstract
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This experimental study evaluated the accuracy of digital models generated by two scanning techniques (intraoral scanner and bench-top scanner) of an experimental model of the upper arch made of polyurethane and the plaster model obtained from this arch. Reference measurements, which the manufacturer demarcated, were performed using surgical simulation software and analyzed for validity and accuracy of the scanning techniques used in all groups (1 to 5). In the validation analysis, all techniques showed to be valid compared to the control group, except the FG measure, which showed statistically significant differences (p<0.05) between groups 1 and 2. Precision was assessed using the intraclass correlation index, and all techniques were highly accurate with an ICC close to 1. Thus, it is concluded that the intraoral scanner and the bench scanner used in this study were reliable compared to the control group and that the two scanner models used presented themselves with high precision.
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4
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ANA CLÁUDIA DE MACÊDO ANDRADE
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IN VITRO AND IN SILICO EVALUATION OF THE ANTINEOPLASTIC ACTIVITY OF S-(-)-PERILLYL ALCOHOL ON EQUAMOUS CELL CARCINOMA OF THE TONGUE
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Advisor : ANTONIO DE LISBOA LOPES COSTA
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COMMITTEE MEMBERS :
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LUCIANA SCOTTI
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ANTONIO DE LISBOA LOPES COSTA
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AURIGENA ANTUNES DE ARAUJO
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CARLOS AUGUSTO GALVAO BARBOZA
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SABRINA GARCIA DE AQUINO
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Data: Mar 31, 2022
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Show Abstract
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Oral squamous cell carcinoma (OSCC) is the most frequent malignant neoplasm of the oral cavity and constitutes a public health problem due to its high incidence and mortality rate caused in many cases by therapeutic failure and tumor resistance. Therefore, the search for new biologically active molecules stands out, such as those found in products of natural origin. This work aims to evaluate the antineoplastic activity of S-(-)-perillyl alcohol (POH) in cell cultures of tongue CEO and to analyze the probable mechanism of action on the antineoplastic activity of this phytoconstituent through molecular docking. For this purpose, two cell lines of tongue CEO were used, HSC-3 and SCC-25. The following groups were analyzed: G0 (control; cells cultured in the absence of POH), G1 (cells treated with 40 μM cisplatin), G2 (cells treated with 0.5 mM POH), G3 (cells treated with 1 .0 mM), G4 (cells treated with 1.5 mM POH) and G5 (cells treated with 3.0 mM POH). Differences between these groups were investigated through the following assays: cell viability (Alamar Blue and Live/Dead assay) and migratory activity (Wound healing). The prediction of the POH action mechanism on cell cycle control molecules were also performed using molecular docking using AutoDock 4.2 and Molegro Virtual Docker, v. 6.0.1. The data was statistically treated by GraphPad Prism 6.0 (GraphPad Software, USA), parametric analysis using Anova test, Tukey post-test and Kruskal-Wallis non-parametric statistical test, followed by Mann-Whitney post-test were adopted for determination of differences between the experimental groups. The significance index considered in this work was 5%. In the analysis of cell viability using Alamar Blue, for the cell line SCC-25, cell viability was significantly reduced in the 40 μM cisplatin, 0.5 mM POH, 1 mM POH, 1.5 mM POH and 3 mM POH groups (p<0.05), at intervals of 24 h and 48 h when compared to the growth control. In turn, in the 72 h interval, only the 0.5 mM POH concentration showed no statistical difference when compared to the control group (p= 0.35). For the HSC-3 cell line, there was a significant decrease in viability in the 40 μM, 1 mM, 1.5 mM and 3 mM cisplatin groups (p<0.01), in the time interval of 24h, 48h and 72h, when compared to growth control. Furthermore, for both strains, the 3 mM POH concentration presented the best results of viability reduction when compared to 40 μM cisplatin, in the intervals of 24 h for SCC-25 and 24 h (p<0.01) and 48h (p<0.01) for HSC-3. In the analysis of cell viability by the Live/Dead assay, for the cell line SCC-25, all experimental groups showed a significant reduction in the percentage of cell viability (p<0.001) when compared to the control group, since, for the HSC line -3, only the 0.5 mM POH group showed no statistically significant difference compared to the control group (p= 0.9663). As for the anti-migratory capacity of POH, for the SCC-25 strain, the 40 μM cisplatin, 0.5 mM POH and 1.0 mM POH groups had a statistically significant reduction in cell migration when compared to the control group, at 12 h, on the other hand, within 24 h, only cisplatin showed anti-migratory activity (p≤ 0.05). For the HSC-3 strain, the 40 μM cisplatin and 1 mM POH groups showed statistical differences compared to the control group (p≤ 0.05), at 12 h and 24 h intervals. The ability of the POH molecule to bind to proteins responsible for cell cycle activation was evaluated using docking models. Among them, the protein GTPase Kras showed the best binding energy (-86.70 kcal/mol), featuring hydrogen bonds with residues THR58 (A) and ASP57 (A) and steric bonds with residues TRY32 (A) and ALA18 ( THE). The evidence from this study supports the idea that POH has antineoplastic activity on the CEO, suggesting that this molecule may be a strong candidate for the development of drugs aimed at the treatment of this pathology.
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5
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KEIVERTON RONES GURGEL PAIVA
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THE INFLUENCE OF THE ABUTMENT ON THE SATISFACTION AND QUALITY OF LIFE OF PATIENTS REHABILITATED BY A METAL-FREE SINGLE CROWN: A CLINICAL TRIAL
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Advisor : PATRICIA DOS SANTOS CALDERON
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COMMITTEE MEMBERS :
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PATRICIA DOS SANTOS CALDERON
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GUSTAVO AUGUSTO SEABRA BARBOSA
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WAGNER RANIER MACIEL DANTAS
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KATIA RODRIGUES REIS
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ANDRE ULISSES DANTAS BATISTA
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Data: Apr 27, 2022
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Show Abstract
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Introduction: The quality of implant-supported rehabilitation in addition to the principles of osseointegration, demands a wider assessment, both from the aesthetic point of view and its impact on the patient's satisfaction and quality of life. Objective: To evaluate objectively and subjectively the aesthetic function, quality of life, and patient's satisfaction wearing metal-free crowns on single implants installed in the smile region using prefabricated titanium (GT) or customized zirconia (GZ) prosthetic abutments. Materials and methods: A nonrandomized controlled clinical trial was performed out with the sample arrangement in a sequenced manner; the first half had a prefabricated titanium abutment (GT) and the second half a zirconia abutment (GZ). All patients received a temporary restoration before the final crown. Aesthetic assessments were performed using the Pink Esthetic Score (PES) and White Esthetic Score (WES), satisfaction was assessed by a Visual Analog Scale (VAS), and quality of life by the Oral Impacts on Daily Performances (OIDP) and the Oral Health Impact Profile (OHIP-14). Results: Twenty-four patients were included, the mean age was 40.03 years. 26 implants were installed, 14 in GT and 12 in GZ, the anterior region had 18 placed implants and the posterior region had 08 implants. The OIDP was positively influenced by the provisional crown in both groups, remaining the same for the final crowns on GZ and increasing on GT after installing the final crowns. On the OHIP-14 all dimensions improved after the provisional crown and further improved with the final crowns. For the satisfaction, an improvement was noticed by the provisional crown, in both groups, and the final crowns bring another increase in satisfaction. Conclusion: The placement of metal-free crowns on single implants can play an important role in the improvement of the patient's satisfaction and quality of life.
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6
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CRISTIANNE KALINNE SANTOS MEDEIROS
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COMPARATIVE ANALYSIS OF BURNING MOUTH TREATMENT WITH LOW-INTENSITY LASER THERAPY AND TRANSCUTANEOUS ELECTRICAL NERVOUS STIMULATION: A RANDOMIZED CLINICAL TRIAL
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Advisor : PATRICIA TEIXEIRA DE OLIVEIRA
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COMMITTEE MEMBERS :
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AMANDA KATARINNY GOES GONZAGA
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ANTONIO ADILSON SOARES DE LIMA
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EMELINE DAS NEVES DE ARAUJO LIMA
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ERICKA JANINE DANTAS DA SILVEIRA
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PATRICIA TEIXEIRA DE OLIVEIRA
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Data: May 30, 2022
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Show Abstract
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Introduction: The management of patients with burning mouth is a challenge in the clinical routine. Objective: To compare the effect of low-level laser therapy (LLLT) and transcutaneous electrical nerve stimulation (TENS) in the treatment of burning mouth. Methodology: Randomized clinical trial, consisting of 25 patients with burning mouth, of which 12 were allocated to the TENS group and 13 to the LLLT group. The two-way analysis of variance (ANOVA) test was used to verify whether there was a significant difference between the times T0, T1, T2 and T3 in relation to symptomatology, investigated through the Visual Analog Scale (VAS), and unstimulated salivary flow with TENS and LLLT interventions. Results: Most partients were female with a mean age of 59.25 years in the TENS group and 62.08 in the LLLT group. The VAS analysis showed a significant intragroup difference (p<0.001), and in the TENS group it occurred between T0xT1, T0xT2 and T0xT3, and in the LLLT group, between T0xT2 and T0xT3. There was a significant difference for VAS between the interaction of times with the analyzed groups (p=0.034), whose difference was found between the time intervals T2 and T3 (p=0.003), in which the VAS score decreased in the LLLT group and increased in the TENS group. The linear trend test in relation to unstimulated salivary flow showed a statistical difference intergroup between T1 and T2 times, since salivary flow increased in the TENS group and decreased in the LLLT group (p=0.052). Conclusion: TENS and LLLT were effective in reducing the symptoms of burning mouth during treatment sessions, and LLLT presented a better VAS score after treatment follow-up. Additionally, none of the therapies employed had adverse effects.
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7
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JANAÍNA LESSA DE MORAES DOS SANTOS
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IMMUNOHISTOCHEMICAL PROFILE OF MARKERS ASSOCIATED WITH EPITHELIAL-MESENCHYMAL TRANSITION IN BENIGN EPITHELIAL ODONTOGENIC LESIONS
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Advisor : LELIA MARIA GUEDES QUEIROZ
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COMMITTEE MEMBERS :
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LELIA MARIA GUEDES QUEIROZ
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PEDRO PAULO DE ANDRADE SANTOS
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AMANDA KATARINNY GOES GONZAGA
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HIANNE CRISTINNE DE MORAIS MEDEIROS
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LEORIK PEREIRA DA SILVA
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Data: Jun 21, 2022
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Show Abstract
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Odontogenic cysts and tumors present a high percentage of the lesions that affect the maxillomandibular complex and some of them may exhibit a more aggressive clinical-biological behavior. In the epithelial-mesenchymal transition (EMT), epithelial cells lose phenotypic properties characteristic of epithelial cells and acquire properties characteristic of mesenchymal cells, including increased motility and invasiveness, through the regulation of central transcription factors and their associated pathways. Thus, the present study aimed to analyze and compare the immunohistochemical expression of proteins involved in the EMT process (Zeb1, E-cadherin, N-cadherin, and vimentin) in benign epithelial odontogenic lesions. The sample consisted of 88 cases of odontogenic lesions, comprising 28 cases of ameloblastoma (AB), 30 of odontogenic keratocyst (OK), and 30 of dentigerous cyst (DC). All specimens submitted to the immunohistochemical technique were evaluated by light microscopy and submitted to the random choice of 5 (five) fields, which were photographed at a 40x magnification. The evaluation of the expression of each marker, based on the analysis in its specific cellular compartment, was performed by multiplying the scores associated with the percentage of immunoreactive cells by the scores related to the intensity of staining. Such a result was defined as low expression or high expression, according to the methodology choosen. The associations were made using the chi-square test and the correlations through the Spearman correlation test. The significance level was set at 5% (p < 0.05). The results were statistically significant in the epidemiological variables of the lesion region, radiographic appearance, and lesion size. In turn, immunohistochemistry showed a positive and moderate correlation between nuclear Zeb1 and membrane E-cadherin, cytoplasmic Zeb1 and membrane E-cadherin, and between cytoplasmic E-cadherin and vimentin in cases of AB; a moderate positive correlation between nuclear Zeb1 and cytoplasmic vimentin, and between cytoplasmic Zeb1 and vimentin in cases of dentigerous cyst. Therefore, it was possible to conclude that Zeb1 may not act directly on the pathways responsible for the development and growth of these studied lesions, and E-cadherin, N-cadherin, and vimentin may be part of a partial EMT process that would occur in the studied benign epithelial odontogenic lesions.
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8
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DÁUREA ADÍLIA CÓBE SENA
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Immunoexpression study of proteins involved in epithelial-mesenchymal transition in salivary gland tumors
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Advisor : LELIA BATISTA DE SOUZA
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COMMITTEE MEMBERS :
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AMANDA KATARINNY GOES GONZAGA
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JAMILE MARINHO BEZERRA DE OLIVEIRA MOURA
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LEAO PEREIRA PINTO
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LELIA BATISTA DE SOUZA
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LEORIK PEREIRA DA SILVA
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Data: Jun 23, 2022
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Show Abstract
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Salivary gland tumors (SGTs) present remarkable clinical and biological complexity; therefore, many studies investigate the events involved in their progression. One of the dynamics involved in the tumor invasion of different types of carcinomas is the epithelial-mesenchymal transition (EMT). In this process, epithelial cells undergo a transition to a mobile mesenchymal state, favoring invasion and metastasis. To acquire this phenotype, epithelial cells reduce the expression of E-cadherin and increase the expression of Twist1, Snail1, vimentin (VM), and matrix metalloproteinases (MMPs). In addition, myofibroblasts, located in the stroma, express smooth muscle alpha-actin (α-SMA) and are known to modulate tumor progression. Therefore, this research analyzed the immunohistochemical expression of some proteins involved in EMT in a series of SGTs cases; correlations among the biomarkers, as well as between the biomarkers and clinicopathological parameters were made. We selected 20 cases of pleomorphic adenoma (PA), 20 cases of mucoepidermoid carcinoma (MEC), 20 cases of adenoid cystic carcinoma (ACC), 10 cases of polymorphous adenocarcinoma (PAC), 10 cases of epithelial-myoepithelial carcinoma (EMC) and 10 cases of carcinoma ex-pleomorphic adenoma (CXPA). E-cadherin, Twist1, and Snail1 were analyzed in tumor parenchyma, observing the percentage of positive cells (PP) using scores ranging from 0 to 4, and the expression intensity (EI), whose scores were ranged from 0 to 3. The evaluation of MMP-9 was performed in tumor parenchyma and stroma, also evaluating PP and IE, both based on scores that ranged from 0 to 3. The labeling for α-SMA and VM was analyzed in stromal cells. Positive cells for α-SMA were counted in 10 fields and the mean was calculated. VM was evaluated qualitatively, using 4 scores according to EI and whether the labeling was diffuse or focal. Obtained data were analyzed using Statistical Package for Social Science, GraphPad Prism, and STATA software. The significance level of 5% was adopted for the statistical tests. Patients were mostly female, with a mean age of 49.8 years; the major salivary glands were the most affected anatomical site, mainly the parotid gland. A lower E-cadherin immunostaining was verified in PAs in comparison to malignant neoplasms of salivary glands (MNSGs). Low immunoexpression of Twist1 and Snail1 was observed in PAs. Regarding the nuclear expression of Twist1, it was found greater expression in malignant neoplasms than in PAs. Furthermore, Twist1 in the nucleus was correlated with cytoplasmic expression of E-cadherin in MNSGs. Regarding clinicopathological parameters, this protein was statistically related to higher chances of death. Twist1 immunoexpression in cytoplasm showed loss of expression in CACs compared to MECs, PACs, and EMCs. Low immunoexpression of Snail1 was evidenced among the MNSGs. However, in the analysis of CACs, greater nuclear expression was observed in the solid variant compared to the others. Expression of MMP-9 in parenchyma showed a positive correlation with cytoplasmic Twist1 and Snail1nuclear in MNSGs. MMP-9 also showed a positive correlation when comparing its immunoexpression in the parenchyma and the stroma. VM was presented as a biomarker to be considered in the clinical evaluation of patients since it showed a significant correlation between greater tumor size and a higher frequency of death. Furthermore, the high expression of this protein appeared as an independent predictive factor for worse overall survival (OS) rates. The evaluation of the rest of the clinicopathological factors showed advanced clinical stages as an indicator of independent prognostic value for lower rates of OS. For disease-free survival, these indicators were the location in the minor salivary gland and the presence of distant metastasis. Our results suggest that the EMT may be related to myoepithelial differentiation in PAs and tumor progression in MNSGs. Also, Twist1 and MMP-9 appear to play a greater role in the scenario of EMT in MNSGs; finally, VM might be used as a prognostic value indicator.
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9
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MOAN JÉFTER FERNANDES COSTA
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IN SILICO AFFINITY BETWEEN ANALGESIC/ANTI-INFLAMMATORY DRUGS AND THE TRANSIENT RECEPTOR POTENTIAL A1 TO PREDICT POTENTIAL PHARMACOLOGICAL MANAGING APPROACHES FOR BLEACHING SENSITIVITY
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Advisor : BONIEK CASTILLO DUTRA BORGES
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COMMITTEE MEMBERS :
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BONIEK CASTILLO DUTRA BORGES
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ISAUREMI VIEIRA DE ASSUNCAO
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MARILIA REGALADO GALVAO RABELO CALDAS
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DÉBORA MICHELLE GONÇALVES DE AMORIM
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LETICIA VIRGINIA DE FREITAS CHAVES
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Data: Jun 27, 2022
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Show Abstract
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Introduction: Reducing in-office tooth bleaching sensitivity represents a challenge for professionals. Researchers have associated the block of the pain receptor TRPA1 with reducing bleaching sensitivity. However, the chemical affinity of analgesic/anti-inflammatory drugs to the TRPA1 needs to be verified. Objective: To perform a virtual screening of multiple drugs (analgesic and anti-inflammatory drugs) to verify chemical affinity for the TRPA1 receptor. Methodology: The crystal structure of the TRPA1 receptor proteins was retrieved from the Protein Data Bank. The SMILES codes of the ligands were extracted from PubChem. The binding energy of the complex was obtained in ∆G - kcal/mol by AutoDock Vina© and replicated in the webservers SwissDock©, Dockthor©, and CbDock©. LigPlus© confirmed the binding sites. Results: Although the receptor antagonists analyzed showed high affinity, codeine and dexamethasone showed regularity among all servers, even showing binding energy values of -7.9 kcal/mol for codeine and -8.1 kcal/mol for dexamethasone. Conclusion: Codeine and dexamethasone may be potential drugs to manage tooth bleaching sensitivity if they reach the dental pulp TRPA1 receptor.
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10
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RAUL ELTON ARAUJO BORGES
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VALIDITY AND RELIABILITY OF THE EPIDEMIOLOGICAL DIAGNOSTIC INSTRUMENT FOR TEMPOROMANDIBULAR DISORDERS- EDI/TMD
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Advisor : PATRICIA DOS SANTOS CALDERON
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COMMITTEE MEMBERS :
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MARGARETE RIBEIRO DA SILVA
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EVELYN MIKAELA KOGAWA
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GUSTAVO AUGUSTO SEABRA BARBOSA
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PATRICIA DOS SANTOS CALDERON
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PAULO CESAR RODRIGUES CONTI
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Data: Aug 15, 2022
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Show Abstract
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Introduction: Temporomandibular disorders (TMD) is a highly underreported oral health problem. In addition, its diagnosis is complex and requires the use of valid and reliable instruments for use in epidemiological studies. Objective: To validate the Epidemiological Diagnostic Instrument for TMD (EDI/TMD), with applicability for national epidemiological studies. Methodology: This is a validation study, internal structure validity through Exploratory Factor Analysis (EFA), reliability (internal consistency; interobserver and intraobserver), and convergent validity of the EDI/TMD were assessed using the Diagnostic Criteria for Temporomandibular Disorders (DC/TMD) as the gold standards. Results: Bartlett’s test (X2 = 734.645; p< 0,001) and the Kaiser–Meyer–Olkin (KMO=0,746) measure of sampling adequacy was obtained. AFE showed tree factors (Muscle TMD, Joint TMD, and Differential Diagnosis) with all items having factor loadings > 0.4, which explained 73.3% of the total variance. Good internal consistency with Cronbach's alpha= 0.775. Reliability ranged from substantial to excellent, reproducibility interobserver (Kappa=0.79-1.00, ICC=0.91-0.97, p< 0.001) and intraobserver (Kappa= 0.75-1.00, ICC=0.80-0.95; p< 0.001). The EDI/TMD total score showed valid and satisfactory diagnostic criteria according to the DC/TMD (Kappa= 0.906; p<0.001), with the ability to differentiate between individuals without and with TMD with a cut-off point of 4.9 (Sensitivity=1.0; Specificity=1.0; AUR=1.0); Mixed TMD with a cut-off point of 14 or more (Sensitivity=0.8; Specificity=1.0; AUR=0.987); and either Muscles TMD (Sensitivity=1.0; Specificity= 0.88; PPV= 0.89; NPV=1.0) or Joint TMD (Sensitivity=0.95; Specificity= 0.87; PPV= 0.83; NPV=0.96), with a cut-off point between 5-13.9, with the diagnosis obtained from the highest score in each factor (Muscles or Joint). Conclusion: The EDI/TMD is a valid and reliable assessment tool, with adequate and satisfactory psychometric properties, capable of diagnosing people with TMD and classifying the subtype of the condition (Muscle, Joint, and Mixed). Finally, the EDI/TMD is an important Brazilian questionnaire that should be used for TMD epidemiological surveys and in oral health services as a faster and simpler screening tool than the DC/TMD.
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11
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FRANCISCO LEONARDO DA SILVA JUNIOR
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EVALUATION OF THE USE OF THE PROBIOTIC LACTOBACILUS CASEI ADJUNCT TO PERIODONTAL THERAPY IN EXPERIMENTAL MODEL OF PERIODONTITIS: A RELATIONSHIP BETWEEN PERIODONTAL AND INTESTINE
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Advisor : ANA RAFAELA LUZ DE AQUINO MARTINS
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COMMITTEE MEMBERS :
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ANA RAFAELA LUZ DE AQUINO MARTINS
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RUTHINEIA DIOGENES ALVES UCHOA LINS
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EULER MACIEL DANTAS
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FERNANDO JOSE DE OLIVEIRA NOBREGA
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SABRINA GARCIA DE AQUINO
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Data: Aug 17, 2022
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Show Abstract
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Periodontitis is a chronic inflammatory condition that can influence the gut microbiota. The gold standard treatment for periodontitis includes scaling and crown-root planing, but in complex cases adjuvant therapies such as probiotics can be used. The use of this adjuvant treatment may contribute to the improvement of periodontal condition and intestinal symbiosis. The aim of this study was to evaluate the effect on periodontal and intestinal inflammation of the use of Lactobacillus casei (LC) adjunct to scaling and root planing (RACR) in Balb/c mice with ligature-induced periodontitis. This study is a preclinical, in vivo, randomized, blinded, placebo-controlled trial consisting of 40 male Balb/c mice. The animals were submitted to periodontitis induction by placing a 4.0 silk suture ligature around the upper right second molar. The sample was divided into 4 groups, each with 10 animals: Group I: Without Periodontitis and without RACR; Group II: With Periodontitis and without RACR; Group III: With Periodontitis and with RACR; Group IV: With Periodontitis and with RACR + administration of LC, by gavage, for 30 days. Cytokine analyzes were performed by the ELISA method in gingival tissue (IL-6), intestinal tissue (IL-1β, IL-6 and IL-10) and blood (IL-1β and IL-6), the blood was also subjected to analysis biochemical (TGO, TGP, urea and creatinine) and differential leukocyte count. Fragments of the large intestine of these animals were collected and analyzed for biomarkers of oxidative stress (SOD, GSH and MDA), acetylcholinesterase (AChE) activity, and the population of Lactic Acid-Producing Bacteria in the animals' feces was counted. The use of LC adjunct to RACR resulted in a reduction in the expression of IL-6 in the gingival tissue of mice with Periodontitis (p < 0.05), for the blood inteleukins (IL-1β and IL-6), there were no differences between the groups (p > 0.05). As for intestinal cytokines, there was a reduction in the expression of IL-10 (p < 0.05), for the groups that presented Periodontitis. Regarding intestinal oxidative stress, the animals in Groups III and IV had a reduction in MDA levels (p < 0.05), for SOD and GSH, there were no significant differences between the 4 groups studied (p < 0.05 ). It is concluded that the use of LC adjunct to RACR in mice with ligation-induced periodontitis can reduce the release of IL-6 in the gingival tissue. Regarding the intestinal effects, two effects were found: The first related to the modulation of the inflammatory response, with the reduction of MDA, in the animals that received periodontal treatment. And the second related to a pro-inflammatory effect, with the reduction of IL-10 expression.
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12
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NATHALIA RAMOS DA SILVA
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Adhesive cementation of glass ceramics for CAD/CAM: effect of the type of adhesive system and long-term aging on the bond strength to resin cement
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Advisor : RODRIGO OTHAVIO DE ASSUNCAO E SOUZA
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COMMITTEE MEMBERS :
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RODRIGO OTHAVIO DE ASSUNCAO E SOUZA
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ADRIANA DA FONTE PORTO CARREIRO
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SAMIRA ALBUQUERQUE DE SOUSA
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DAYANNE MONIELLE DUARTE MOURA
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RENATA MARQUES DE MELO
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Data: Aug 24, 2022
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Show Abstract
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The aim of this study is to evaluate the effect of the type of adhesive system and aging on the wettability and bond strength of glass ceramics to resin cement. Lithium silicate (SL - Celtra Duo, Dentsply Sirona), feldspathic (FD - Vita Mark II, VITA Zahnfabrik) and polymer-infiltrated ceramic (PIC - Vita Enamic, VITA Zahnfabrik) blocks were sectioned (N=96, 10 x 12 x 2 mm), included and randomly divided into 24 groups according to the factors: “Ceramics” (SL, FD and PIC), “Adhesive” (CONT–control without adhesive; C2P–conventional 2-step adhesive; C3P–adhesive conventional 3-step; UNV–universal adhesive) and “Aging” (SE-non-aged; CE-aged). After the ceramic treatment, cylinders (n=15, Ø = 2 mm and height = 2 mm) of resin cement (RelyX Ultimate, 3M ESPE) were made (4 cylinders/block) and half of the samples were stored in distilled water at 37°C for 24h (SE) and half were subjected to thermocycling 10,000 cycles and storage in water at 37°C for 18 months (EC). Then, the samples were submitted to the shear bond strength test with wire (RUC - 100 KgF, 1 mm/min), failure analysis, contact angle measurement and interface analysis by means of scanning electron microscopy (SEM). RUC (MPa) and contact angle data were analyzed by 2-way ANOVA and 1-way ANOVA, respectively, and Tukey (5%). Weibull analysis was performed for RUC. 2-way ANOVA revealed that the “Adhesive” factor was significant only for SL (p<0.0001) and FD (p=0.0079) ceramics. The Adhesive/Aging interaction (p=0.0015) and “Aging” (p=0.0008) were significant only for SL. For SL, after aging, the CE_UNV (22.18± 7.74CD) and CE_C2P (17.32± 5.86D) groups were lower than the control (30.30± 6.11A). Only the UNV group showed a significant decrease after aging. For FD, C2P had the lowest RUC in relation to the other groups. PIC showed similarity of RUC between all groups. The UNV contact angle presented the lowest value for SL (55.27°) and FD (51.34°). For PIC, C3P presented the highest angle (63.00°) and CONT the smallest (49.83°). Therefore, the application of an adhesive was not beneficial for ceramic-cement adhesion. Furthermore, the use of C2P and UNV after silanization of SL and C2P ceramics for FD should be evaluated with caution, as the bond strength was reduced compared to the control group after aging.
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13
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MARCELO LEITE MACHADO DA SILVEIRA
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Evaluation of cardiovascular alterations and catecholamines serum concentration after oral surgery in patients receiving local anesthetics with adrenergic vasoconstrictor.
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Advisor : ADRIANO ROCHA GERMANO
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COMMITTEE MEMBERS :
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ADRIANO ROCHA GERMANO
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WAGNER RANIER MACIEL DANTAS
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EDUARDO DIAS DE ANDRADE
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FRANCISCO SAMUEL RODRIGUES CARVALHO
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RICARDO JOSÉ HOLANDA VASCONCELOS
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Data: Aug 31, 2022
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Show Abstract
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Objectives: A randomized controlled clinical trial was developed to evaluate the cardiovascular effects of local anesthetics with vasoconstrictors (LAVC) in healthy and hypertensive patients undergoing teeth extraction with lidocaine 2% with epinephrine 1:100.000. Materials and methods: 20 patients were divided into control (CG – normotensive patients) and experimental groups (EG – hypertensive patients). The variables analyzed were heart rate (HR), oxygen saturation (O2S), systolic and diastolic blood pressure (sysBP and diasBP), serum catecholamines concentration (dopamine, epinephrine, and norepinephrine), ventricular and supraventricular extrasystoles (VES and SVES respectively), and ST segment depression. Data was obtained in three different moments (initial, trans and final). Blood samples were taken to measure the catecholamines and a Holter device was used to measure data from the electrocardiogram including a 24-hour postoperative evaluation period. The Mann-Whitney test was used to identify differences between the two groups and the Friedman test with the adjusted Wilcoxon post test were used for intragroup evaluation for repeated measures. Results: The EG presented a lower O2S in the initial period (p = 0,001) while the sysBP showed a statistical difference for the three evaluation periods with the EG presenting the highest values. The VES where higher for the EG during the 24-hour postoperative evaluation period (p = 0,041). The SVES and the serum catecholamines showed were similar between the groups. The intragroup analysis revealed significant statistical difference for the sysBP in the EG with the trans period presenting the highest measurements. The extrasystoles evaluation showed that the 24-hour postoperative period presented most events with only the CG not presenting statistical difference for the variable VES during this period (p = 0,112). No ST segment depression was noticed for both groups. Conclusions: Teeth extraction with LAVC can be safely executed in hypertensive patients. Blood pressure should be monitored in these patients since the sysBP presented significant differences during the surgical procedures. Cardiac arrhythmia and the serum catecholamines concentration levels seem not to be altered by the surgical procedure. Also, serum catecholamines to not influence cardiovascular changes in this type of surgery.
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14
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BRUNA KATHERINE GUIMARÃES CARVALHO
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STABILIZING SPLINTS MANUFACTURED BY DIGITALMETHOD: IN VITRO MICROBIOLOGICAL TEST AND FRACTURE RESISTANCE
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Advisor : ERIKA OLIVEIRA DE ALMEIDA FREITAS
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COMMITTEE MEMBERS :
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ERIKA OLIVEIRA DE ALMEIDA FREITAS
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GUSTAVO AUGUSTO SEABRA BARBOSA
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MARIA REGINA MACEDO COSTA
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Paulo Cézar Simamoto Júnior
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SHEILA RODRIGUES SOUSA PORTA
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Data: Sep 28, 2022
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Show Abstract
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CAD/CAM technology allowed the stabilizing splints manufacture, however there are gaps about these devices' behavior in face of mechanical and biological variables that are still unknown. Thus, the objective of this project was to verify if the resins used in digital technology of milled and printed stabilizer splints can influence fracture resistance and microorganism adhesion, in relation to the resin applied in manufacture of conventional thermopolymerizable splints. The method included mechanical and microbiological steps, each one composed by Conventional (CG) (n=30), Milled (MG) (n=30) and Printed (PG) (n=30) groups, according to the method of preparation, each one having three subgroups (n=10) that varied the specimen thickness from 1 to 3 mm. In the mechanical step, the samples, in the form of 65 mm bars, were tested for fracture resistance in a universal testing machine, with load cell of 500 kgf and speed of 1 mm/min; in the microbiological step, the shape was in form of 15 mm diameter discs, subjected to micro-organism adhesion to the surface with exposure to Streptococcus mutans and kept for 24 hours at 35 °C in oven for successive CFU counting. Data were stored in SPSS 22.0 and statistical analysis used ANOVA and Tukey's post hoc. In the results of mechanical step ANOVA had identified statistically significant differences between the groups (p≤0,005), showing CG and MG had presented better results. Tukey´s post hoc had considered statistically significant difference between MG and PG (p=0,031), listing MG as superior. For the microbiological step, they did not show statistical differences between the groups (p>0.005) in any analysis. It was observed milled resins described superior results and/or close to the conventional technique one, for fracture resistance; and printed resins are superior in terms of microorganisms’ surface adhesion.
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15
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RODRIGO FALCÃO CARVALHO PORTO DE FREITAS
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Complete dentures digital workflow:
Biomechanical properties and development of a novel workflow
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Advisor : ADRIANA DA FONTE PORTO CARREIRO
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COMMITTEE MEMBERS :
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ADRIANA DA FONTE PORTO CARREIRO
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RODRIGO OTHAVIO DE ASSUNCAO E SOUZA
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SANDRA LÚCIA DANTAS DE MORAES
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TÚLIO PESSOA DE ARAÚJO
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VINICIUS DUTRA
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Data: Oct 21, 2022
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Show Abstract
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Complete denture (CD) rehabilitation is the most usual prosthodontic treatment for patients with edentulism. Currently, CDs are mainly designed and fabricated using conventional methods, which involve a broad series of clinical and laboratory procedures. In this context, new digital features like subtractive and additive manufacturing can promote breakthroughs by reducing the time and cost of making CDs. Thus, this study aims to investigate surface characteristics (roughness and contact angle), mechanical properties (mini-flexural strength), and biofilm adhesion on computer-aided design/computer-aided manufacturing (CAD-CAM) PMMA polymer, and three-dimensional (3D) printed resin for denture´s base fabrication as well as to propose an innovative workflow. For in vitro analysis, a total of 60 discs and 40 rectangular specimens were fabricated from one CAD-CAM pre-polymerized PMMA disc (GM), one 3D-printed (GP), and two conventional heat-polymerized (GCL and GCV) materials for denture base fabrication. Roughness was determined by the Ra value; the contact angle was measured by the sessile drop method; the mini-flexural strength test was a three-point bending test while the biofilm formation inhibition behavior was analyzed through C. albicans adhesion. The data were analyzed using descriptive and analytical statistics (α = 0.05). Results showed that 3D-printed specimens presented the highest surface roughness (Ra: 0.317 ± 0.151 μm) and lowest mini-flexural strength values (57.23 ± 9.07 MPa) and the CAD-CAM PMMA group showed the lowest C. Albicans adhesion (log CFU/mL: 3.74 ± 0.57) and highest mini-flexural strength mean (114.96 ± 16.23 MPa). There was no statistical difference between CAD-CAM pre-polymerized resin and conventional groups for roughness, contact angle, and mini-flexural strength. In turn, the presented novel workflow for complete dentures fabrication reached a three appointments protocol in which preliminary impressions are made in the 1st session, all together to maxillary registration of lip support, occlusal plane, and reference lines for teeth arrangement. A trial denture is manufactured and evaluated by the 2nd appointment through conventional or CAD-CAM procedures, allowing esthetics evaluation, final impression, and maxillomandibular relationship record and providing precise references for final dentures fabrication. Based on the findings of this in vitro study, CAD-CAM milled PMMA polymers presented surface and mechanical properties similar to conventional resins and show improved behavior preventing C. albicans adhesion, while 3D printed acrylic specimens exhibited the lowest flexural strength, and highest surface roughness. The proposed device for CD´s fabrication clinical procedures reached feasibility to be used either by conventional workflow or by CAD-CAM technology and, by simplifying available techniques, this protocol could achieve reliability and predictability to produce precise complete dentures with reduced working time.
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16
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CAIO CÉSAR DA SILVA BARROS
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Study of cell cannibalism and epigenetic modification of histone H3 in giant cell lesions
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Advisor : ERICKA JANINE DANTAS DA SILVEIRA
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COMMITTEE MEMBERS :
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CRISTIANE HELENA SQUARIZE
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ERICKA JANINE DANTAS DA SILVEIRA
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KARUZA MARIA ALVES PEREIRA
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LELIA MARIA GUEDES QUEIROZ
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MARCIA CRISTINA DA COSTA MIGUEL
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Data: Nov 1, 2022
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Show Abstract
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Background: Central giant cell granuloma (CGCG) of the jaws is a benign lesion that exhibits varied clinical behavior, being classified as non-aggressive or aggressive. This research aimed to morphologically evaluate the cell cannibalism and the immunohistochemical expression of the acetylation of histone H3 lysine 9 (H3K9ac) and ING5 in 19 cases of peripheral giant cell granuloma (PGCG), 38 cases of CGCG (19 non-aggressive cases and 19 aggressive cases) and in 19 cases of giant cell tumor (GCT) of bone, as well as to analyze the association of cell cannibalism and this immunoexpression with the clinical behavior of these lesions. Methods: Cell cannibalism analysis was performed through the quantification of cannibal multinucleated giant cells (CMGC), while the analysis of H3K9ac and ING5 immunoexpression was performed quantitatively and semiquantitatively, respectively, in mononuclear cells and quantitatively in multinucleated giant cells (MGC) and CMGC. Data analysis was performed using Student's t-test and Spearman's rank correlation coefficient. Results: A significant great amount of CGMC was observed in CGCG aggressive compared to non-aggressive CGCG (p = 0.044). There were no significant differences in the CMGC index between PGCG and non-aggressive CGCG (p = 0.858) and between aggressive CGCG and GCT of bone (p = 0.069). CGCG that exhibited rapid growth and tooth displacement and/or root resorption showed a great amount of CMGC (p = 0.035; p = 0.041, respectively). Aggressive CGCG showed higher immunoexpression of H3K9ac (p < 0.0001) and ING5 in MGC and CMGC (p < 0.05; p < 0.0001, respectively) when compared to non-aggressive CGCG. There was no difference in H3K9ac and ING5 immunoexpression between aggressive CGCG and GCT of bone (p > 0.05). A higher frequency of score 4 of ING5 was observed in mononuclear cells in all lesions. H3K9ac and ING5 immunoexpression were associated with aggressive characteristics in CGCG (p < 0.05). Conclusions: Aggressive CGCG shows a high CMGC index when compared to non-aggressive CGCC. Thus, its quantification can help to predict CGCG clinical behavior. The significantly higher H3K9ac and ING5 immunoexpression may reflect greater clastic activity and cell cannibalism induction in aggressive CGCG and, consequently, be associated with greater aggressiveness in these lesions.
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17
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ALINE DE SOUSA BARBOSA FREITAS PEREIRA
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Metformin Hydrochloride-Loaded PLGA Nanoparticle in Periodontal Disease Experimental Model Using Diabetic Rats
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Advisor : AURIGENA ANTUNES DE ARAUJO
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COMMITTEE MEMBERS :
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AURIGENA ANTUNES DE ARAUJO
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BRUNO CESAR DE VASCONCELOS GURGEL
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FRANCISCO LEONARDO DA SILVA JUNIOR
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JEAN NUNES DOS SANTOS
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RAFAEL RODRIGUES LIMA
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Data: Nov 3, 2022
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Show Abstract
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There is a correlation between diabetes and periodontal disease, and Metformin (MET) in addition to controlling glycemic levels, has shown anti-inflammatory effects and decreased periodontal bone loss. When MET is delivered to a system of nanoparticles of biodegradable polymers, the advantage of increasing therapeutic efficacy can be presented. Objectives: This study consisted of evaluating the anti-inflammatory effects, bone loss and in vitro/in vivo availability of a polylactic-co-glycolic acid (PLGA) nanoparticle associated with MET in a ligature-induced periodontitis model. Materials and methods: The PLGA loaded with different doses of MET was characterized by its mean diameter, particle size, polydispensation index and trapping efficiency. Male Wistar rats were randomly divided into control and experimental groups with different doses of MET associated or not with PLGA, which received different treatments orally. Samples of maxilla and gingival tissue were used to evaluate bone loss and inflammation, by means of micro computed tomography (µCT), histopathological, immunohistochemistry, analysis of inflammatory cytokines and gene expression of proteins by quantitative RT-PCR. For the in vitro release assay (free of Met or PLGA + Met-12.5 mg/mL for 360 min), static Franz vertical diffusion cells were used. For analysis of availability, blood samples were collected at different time intervals, and analyzed by high performance liquid chromatography coupled to mass spectrometry (HPLC-MS/MS). Results: The mean diameter of MET-loaded PLGA nanoparticles was in a range of 457.1 ± 48.9 nm (p <0.05) with a polydispersity index of 0.285 (p <0.05), Z potential of 8.16 ± 1.1 mV (p < 0.01) and trapping efficiency (EE) of 66.7 ± 3.73. Treatment with MET 10 mg/kg + PLGA showed a low concentration of inflammatory cells, weak immunostaining for RANKL, cathepsin K, OPG and osteocalcin and decreased levels of IL-1β and TNF-α (p < 0.05), increase in AMPK gene expression (p <0.05) and decrease in NF-κB p65, HMGB1 and TAK-1 (p <0.05). PLGA + Met 10 mg/kg was released in the in vitro assay suggesting a kinetic model of parabolic diffusion with a release profile of 100% in 10 h by controlled diffusion. The in vivo assay showed the apparent volume of distribution Vz/F (PLGA + Met 10 mg/kg, 40971.8 mL/kg vs. Met 100 mg/kg, 2174.58 mL/kg) and the mean residence time MRTinf (PLGA + Met 10 mg/kg, 37.66 hr vs. Met 100 mg/kg, 3.34 hr). Conclusion: MET loaded PLGA decreased inflammation and bone loss in periodontitis in diabetic rats. The formulation modifies pharmacokinetic parameters, such as apparent volume of distribution and mean residence time. PLGA + Met 10 mg/kg had a slower elimination rate compared to Met 100 mg/kg.
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18
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ARETHA HEITOR VERISSIMO
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FACTORS ASSOCIATED WITH THE INTEREST OF USERS OF COMPLETE MANDIBULAR PROSTHESIS IN IMPLANT SUPPORTED THERAPY AND IMPACT OF REHABILITATION WITH OVERDENTURE WITH SINGLE IMPLANT
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Advisor : ADRIANA DA FONTE PORTO CARREIRO
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COMMITTEE MEMBERS :
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ADRIANA DA FONTE PORTO CARREIRO
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ERIKA OLIVEIRA DE ALMEIDA FREITAS
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GUSTAVO AUGUSTO SEABRA BARBOSA
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FERNANDA FAOT
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ANDRE ULISSES DANTAS BATISTA
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Data: Nov 9, 2022
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Show Abstract
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This study aimed to identify the factors that influence the decision to replace a conventional mandibular complete denture by treatment with dental implants, as well as to evaluate satisfaction, quality of life and masticatory performance (MP) after rehabilitation with overdentures with a single implant in adapted and not adapted to conventional mandibular complete denture (PT). Initially, a cross-sectional study was carried out with 117 users of conventional complete dentures (PTs), who were evaluated for outcomes related to the denture (number of anterior mandibular PTs, time of use of the current denture, whether the dentures were made by a dentist and regarding regular use of the mandibular prosthesis) and regarding the period of mandibular edentulism, mandibular bone height and the patient's willingness to undergo implant therapy. Among these patients, 22 patients who were interested in undergoing therapy with implants were selected, being allocated by pairing into 2 groups: adapted to the mandibular prosthesis (PTA Group - "adapted to mandibular PT") and non-adapted (PTN Group - " not adapted to mandibular PT”). In each patient, a single implant was installed in the midline and after the osseointegration period, the mandibular prostheses were converted into overdentures. Masticatory performance was evaluated by the sieve method, mandibular bone height by paranoid radiographic measurement, satisfaction by quantitative scale with a validated questionnaire and the impact of oral health on quality of life by the OHIP-Edent-19 questionnaire. The chi-square test was used for data analysis and the prevalence ratios adjusted by means of multivariate Poisson regression, with a confidence interval of 95% in the second, for the statistical analysis in each group and between groups, non-response tests were used. -Wilcoxon and Mann-Whitney parameters, with a significance level of 5%. Seventy-eight participants (66.7%) were interested in implant-supported mandibular PT, mean age 65.68±6.38, predominantly female.PM was not influenced by the choice of mandibular implant-supported PT. Preference for mandibular implant-supported PT was correlated with longer experience in mandibular PT (p=0.021) and was significantly associated with dissatisfaction regarding retention (p=0.005). After the implant intervention, all non-adapted patients became adapted. There was no difference between PTA and mandibular PTN for OHIP-Edent (p=0.276) and PM (p=0.222), satisfaction was significant only for the criterion “comfort in the lower arch” (p=0.043). For pre- and post-treatment comparisons with overdentures, the median total OHIP-Edent decreased significantly in both groups. In the intragroup comparison, this reduction was significant in PTA only for “functional limitation” (p=0.026), and in PTN in almost all domains, except “social dysfunction” and “disability” (p>0.05). There was a statistically significant increase in overall satisfaction from 75.41 to 90.25 (p=0.012) in PTN and from 76.10 to 90.50 (p=0.007) in PTA. The parameters “chewing”, “adaptation”, “retention” and “comfort” in the lower arch were significantly different in both groups, and “gustation”, “phonation” and “pain” in the lower arch were significant only for the group not adapted (p<0.05). There was a significant difference for PM in PTN (p=0.002) and PTA (p=0.047) when comparing the type of rehabilitation. There was no correlation between PM and OHIP before and after rehabilitation (p>0.05). It can be concluded that the previous experience with conventional mandibular PT and the dissatisfaction with the retention of these, influence the will of rehabilitation with mandibular implant-supported overdenture, as well as, rehabilitation with overdenture on single implant presents itself as an alternative for patients not adapted to mandibular conventional PT, helping to accept the use of mandibular prosthesis, as well as proving a positive effect on satisfaction, quality of life and masticatory performance.
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19
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SAMUEL BATISTA BORGES
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COMPARISON OF TWO SURGICAL TECHNIQUES FOR ROOT COVERAGE OF SINGLE GINGIVAL RECESSION
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Advisor : BRUNO CESAR DE VASCONCELOS GURGEL
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COMMITTEE MEMBERS :
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ANA RAFAELA LUZ DE AQUINO MARTINS
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BRUNO CESAR DE VASCONCELOS GURGEL
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DANIELA DA SILVA FEITOSA
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KARYNA DE MELO MENEZES
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RENATO VASCONCELOS ALVES
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Data: Nov 11, 2022
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Show Abstract
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The increasing demands of patients for the treatment of gingival recessions bring up clinically significant therapeutic issues, requiring professionals to constantly improve in less invasive and more predictable surgical techniques. AIM: To compare two root coverage techniques to treat single, unilateral, type 1 gingival recessions. METHOD: This parallel, randomized, double-blind clinical trial evaluated individuals with single, unilateral, type 1 gingival recessions 1 (RT1), who underwent root coverage procedure with subepithelial connective tissue graft associated to a coronally advanced flap (control group) or a tunnel technique (test group). The main parameters evaluated were probing depth (PD), bleeding on probing (BoP), clinical attachment level (CAL), gingival recession (GR), height of keratinized tissue (KTH), gingival thickness (GT), percentage of root coverage (RC) and gingival phenotype (GP), in addition to patient-reported outcome measures (PROMs), such as postoperative pain, cervical dentin hypersensitivity (CDH), esthetics, degree of satisfaction and quality of life, intra and intergroup, throughout 06 months follow-up. Data were statistically analyzed using paired Student t-test, t-test for independent samples, Chi-square, McNemar, Split-Plot Analysis of Variance with post hoc t-test (α = 5%). RESULTS: 46 subjects completed this study (control: 23; test: 23). Surgery time was longer for the test group (control: 40min ± 5.6; test: 51min ± 5.9; p = 0.041). Statistically significant reductions for GR and significant gain for CAL, KTH and GT were observed in the intragroup analysis, in both treatment groups, however, without differences between techniques. The CR increased significantly, but no intergroup differences were observed (control: 89.2%; test: 86.5%; p = 0.069). Intragroup analysis revealed a change in GP (control: 95.65%; test: 91.3%; p < 0.001). Both treatment protocols reduced postoperative pain and CDH and improved esthetics, satisfaction and quality of life (p < 0.001), with no differences between the techniques over time. CONCLUSION: Both treatments showed similar clinical efficacy in terms of root coverage and improvement in PROMs.
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20
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ANA MARGARIDA DOS SANTOS MELO
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In vitro evaluation of the base/top hardness ratio and push-out bond strength between a diatomite-containing composite resin and a bulk-fill composite resin
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Advisor : ISAUREMI VIEIRA DE ASSUNCAO
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COMMITTEE MEMBERS :
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ISAUREMI VIEIRA DE ASSUNCAO
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MARIA CRISTINA DOS SANTOS MEDEIROS
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MARILIA REGALADO GALVAO RABELO CALDAS
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JOSELÚCIA DA NÓBREGA DIAS
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REINALDO DIAS DA SILVA NETO
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Data: Nov 14, 2022
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Show Abstract
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After the advent of bulk-fill resins, characterized by their use in a single increment, the restorative composite containing diatomite in its inorganic content appeared, marketed as Zirconfill®. However, the scarcity of data that scientifically validate the benefits of diatomite for the physical properties of this composite resin makes it imperative to carry out new studies, especially regarding the use of Zirconhill in semi-direct restorations, whose indication requires materials that support masticatory loads. Objective: To evaluate in vitro the base/top hardness ratio (B/T) and push-out bond strength (RU) between a composite resin containing diatomite and a bulk-fill composite resin after 24 hours and after 6 months of aging. in water. Materials and methods: Twenty-eight bovine incisors were used to produce conical dentinal cavities (4.8 mm major x 2.8 mm minor diameter x 4 mm depth) and C factor of magnitude 3.1. These cavity preparations (n=28) were restored with composite resins Filtek One Bulk Fill (BF) (3M ESPE) or Zirconfill (ZF) (BM4) through the semi-direct technique using the Scotchbond universal adhesive system (3M ESPE) and cement dual resin Relyx Ultimate (3M ESPE). The B/T analysis was performed with the aid of a Vickers microhardness tester and the RU was performed using the push-out test in a universal testing machine. All samples were stored in distilled water at 37 °C for 24 hours or 6 months. The B/T and RU data were submitted to one- and two-way analysis of variance (ANOVA), respectively, with Tukey's post-test (p <0.05). Results: There was no statistically significant difference between the composite resins in B/T. For the RU, analyzing each aging time separately, the ZF composite resin showed higher values than the BF resin. However, analyzing over time, there was no statistically significant difference for any resin.
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21
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LIDYA NARA MARQUES DE ARAÚJO
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BEHAVIOR OF PERI-IMPLANT TISSUES AFTER SINGLE IMPLANT REHABILITATION IN AESTHETIC AREA COMPARING PREFABRICATED VERSUS CUSTOM PROSTHETIC ABUTMENTS
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Advisor : BRUNO CESAR DE VASCONCELOS GURGEL
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COMMITTEE MEMBERS :
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BRUNO CESAR DE VASCONCELOS GURGEL
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EULER MACIEL DANTAS
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MICHEL REIS MESSORA
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LUANA MARIA MARTINS DE AQUINO
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MARIANA LINHARES ALMEIDA
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Data: Nov 25, 2022
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Show Abstract
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The esthetics and stability of the soft tissue and bone around the implant is a critical component to the long-term success of the implant and can be influenced by factors such as the type of prosthetic connections. OBJECTIVE: To compare the behavior of the peri-implant tissue of single implant-supported crowns in the anterior aesthetic area using prefabricated titanium prosthetic abutments (G1) and customized zirconia prosthetic abutments (G2). METHODOLOGY: In this controlled, blinded, non-randomized clinical trial, 30 single cemented anterior implants were sequentially allocated: 15 implants rehabilitated on implant system with a prefabricated titanium prosthetic abutment (G1) and 15 on implant system with customized zirconia prosthetic abutment (G2). The behavior of the peri-implant tissue was evaluated at T0 (beginning of tissue conditioning), T1 (end of tissue conditioning), T2 (7 days after final cementation), T3 (6 months after final cementation). Visible Plaque Index (VPI), Gingival Bleeding Index (GBI), Bleeding on Probing (BoP), Probing Depth (PD), Interdental Papilla Height/Width Ratio (PH/PW), Keratinized Mucosa Width (KM), Gingival Thickness (PT), Periodontal Phenotype (PF), Gingival Recession (GR), radiographic distance from the bone crest to the contact point and Pink Esthetic Score (PES) were registered. The results were analyzed with the following statistical tests: Friedman, Mann-Whitney and Chi-square/Fisher's Exact test with a significance level of 5%. RESULTS: No statistically significant differences were observed between groups for any of the clinical parameters in any, (p>0.05). However, intragroup analysis showed a statistical reduction in VPI between T1 and T2 only for G2 (p<0.05). There was a significant decrease between T0 and T3 for GT (1.67 mm - 1.47 mm in G1, and 1.70 mm -1.47 mm in G2) and increase in the ratio PH/PW (0.56 - 0.80 in G1 and 0.70-0.83 in G2) in both groups (p<0.0001). PES also increased significantly for both groups (9 – 12 in G1 and 7 – 12 in G2) from T0 to T1 and remained high in the other periods. CONCLUSION: The results showed that the prosthetic abutments used did not impact differences in clinical and aesthetic variables related to the behavior of peri-implant tissues during the study. Therefore, the decision to select components an aesthetic area should be based on aspects such as cost, workflow and timing.
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22
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ONDINA KARLA MOUSINHO ROCHA TORRES
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INFLUENCE OF THE HIPPO PATHWAY IN ORAL TONGUE SQUAMOUS CELL CARCINOMA
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Advisor : MARCIA CRISTINA DA COSTA MIGUEL
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COMMITTEE MEMBERS :
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ERICKA JANINE DANTAS DA SILVEIRA
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HELLEN BANDEIRA DE PONTES SANTOS
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JOABE DOS SANTOS PEREIRA
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LUIZ ARTHUR BARBOSA DA SILVA
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MARCIA CRISTINA DA COSTA MIGUEL
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Data: Dec 5, 2022
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Show Abstract
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Oral tongue squamous cell carcinoma (OTSCC) has high morbidity and mortality rates. Despite the progress made in this area, researchers continue to search for molecular biomarkers that have predictive value in the prognosis of patients and allow the development of new therapeutic strategies. In this context, several studies have highlighted the role of the Hippo pathway for this purpose. Therefore, this research aimed to evaluate whether the proteins related to the Hippo pathway, LATS2 and YAP1, have some influence on the OTSCC biological behavior. The sample consisted of 26 OTSCC cases and 8 normal oral mucosa cases as control. To morphologically assess the OTSCC, the gradations proposed by the WHO (2005) and by Almangush et al. (2014) were performed. The immunohistochemical profile of LATS2 and YAP1 was evaluated by scores (0-3), based on their immunoexpression in intracellular location (nucleus and/or cytoplasm) and epithelial distribution. Pearson's Chi-square, Fisher's Exact, Mann-Whitney, and Kruskal-Wallis statistical tests were performed for the analysis of the studied parameters. Survival analysis was performed using the Kaplan-Meier method and the log-rank test. For all evaluations, values with p<0.05 were considered significant. High expression of LATS2 was observed both in normal oral mucosa and in most OTSCC, with no statistically significant difference. It was also possible to observe the increase in YAP immunoexpression in cases of OTSCC compared to the normal oral mucosa (p<0.001). The high expression of both proteins in most OTSCC suggests that other signaling pathways, in addition to regulating through LATS2, may be inducing the nuclear YAP expression in these tumors. It was also found that the LATS2 low expression was associated with lower rates of disease-free survival (p=0.039). Furthermore, YAP high expression was found associated with the BD model's high-risk classification (p=0.034), suggesting this protein immunoexpression may be associated with EMT and cell invasion in OTSCC. Therefore, it is concluded that the Hippo pathway can influence the OTSCC biological behavior.
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23
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LARISSA SANTOS AMARAL ROLIM
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IMMUNOEXPRESSION ANALYSIS OF MARKERS OF THE EPITHELIAL-MESENCHYMAL TRANSITION IN SPECIMENS AND CELL LINE OF ADENOID CYSTIC CARCINOMA
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Advisor : LEAO PEREIRA PINTO
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COMMITTEE MEMBERS :
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LEAO PEREIRA PINTO
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LELIA BATISTA DE SOUZA
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ROSEANA DE ALMEIDA FREITAS
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ROGÉRIO MORAES DE CASTILHO
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HELLEN BANDEIRA DE PONTES SANTOS
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Data: Dec 13, 2022
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Show Abstract
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Adenoid Cystic Carcinoma (ACC) characterized by persistent and invasive growth, with a high tendency to local recurrences and distant metastases. It is believed that the mechanism responsible for tumor progression is based on the collective or individual cell invasion, kwon as Epithelial-Mesenchymal Transition (EMT), where occur a decrease in the expression of epithelial biomarkers, such as E-cadherin, and increased expression of mesenchymal biomarkers, such as Twist, Snail and Vimentin. Thus, the present research aims to conduct a research on immunoexpression of EMT transcription factors in ACC specimens and their influence on migratory potential and ability to form tumorsphere in an ACC cell line, through exposure to cisplatin. Expressions of Twist, Snail, E-cadherin and Vimentin were evaluated in 20 tissue specimens of ACC in a quantitative and semi-quantitative manner and analyzed for cellular compartment. In order to look for an association with clinicopathological parameters, survival and two histopathological grading systems, the cases were divided into low expression and high expression, with a score of 4 as the cutoff point considered. In the in vitro assays, the staining of EMT markers by immunofluorescence using cisplatin (stock: 10mg/ml; IC50: 4μg/m), in migration assay (wound healing) and in tumorsphere assay were evaluated. There were no significant associations between clinicopathological parameters and grading systems (p>0.05). The immunohistochemical analysis revealed significant associations between the immunoexpression of vimentin, which was statistically significant when compared with the clinical variables: presence of lymph node metastasis (p=0.020), distant metastasis (p=0.007), late clinical staging (p=0.008) and death (p=0.004). The statistical analysis showed no significant associations between immunohistochemical variables and specific survival and disease-free survival (p>0.05). In in vitro assays, the migratory capacity of cells significantly decreased in the first 6h (p=0.050) and 12h (p=0.050) after exposure to chemotherapy. In immunofluorescence, the immunopositivity of Twist (p<0.001), Snail (p<0.001) and Vimentin (p<0.001) was significantly decreased when exposed to cisplatin. In the tumorsphere assay, there was a statistically significant decrease in the types of spheres when compared between the vehicle and cisplatin groups (holospheres: p=0.034; merospheres: p=0.050; paraspheres: p=0.050) and the number of paraspheres was significantly higher than merospheres (p=0.001) and holospheres (p=0.001) in the vehicle group. Through the results of this study, it was possible to conclude that EMT is present in an expressive, however not in a significant, way in the carcinogenesis of ACC. We also observed that ACC cells that exhibit EMT markers are sensitive to the chemotherapy drug cisplatin, affecting the expression of these markers, their migration and the formation of tumorspheres.
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24
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AMANDA FELIX GONÇALVES TOMAZ
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Correlation between the maturation stages of the cervical vertebrae
and the midpalatal suture
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Advisor : PATRICIA TEIXEIRA DE OLIVEIRA
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COMMITTEE MEMBERS :
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PATRICIA TEIXEIRA DE OLIVEIRA
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JOSE SANDRO PEREIRA DA SILVA
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SERGEI GODEIRO FERNANDES RABELO CALDAS
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HANIERI GUSTAVO DE OLIVEIRA
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VÂNIO SANTOS COSTA
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Data: Dec 21, 2022
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Show Abstract
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Introduction: The midpalatal suture maturation (MPSM) is an important parameter to predict the rapid maxillary expansion (RME) success, but a cone-beam computed tomography is required for its evaluation. A correlation between both the MPSM and cervical vertebrae (CVM) would allow predicting the success of rapid maxillary expansion by means of lateral cephalograms. Objective: To correlate MPSM stages with CVM stages using cone-beam computed tomography (CBCT). Material and methods: A total of 195 CBCTs of skull of individuals of both sexes aged over 5 years old will be analyzed. The CBCT exams will be collected from the documentation file of Clínica Radiologia Odontológica Conceito, located at Natal, Rio Grande do Norte. An examiner will withhold the personal informations of patients and randomly assign the CBCT exams based on an electronically generated numerical sequence. Two other previously calibrated examiners will blindly and independently perform the subjective assessment of MPSM and CVM in each CBCT exam. For this, initially, standardization of head position of participants will be carried out in the CBCT exams. The MPSM stage will be assessed by observing the maxilla in the axial view. Depending on the anatomy of the midpalatal suture, maturation will be classified as A, B, C, D or E. On the other hand, the CVM stage will be assessed in the sagittal view of the cervical vertebrae. Depending on the morphology of C2, C3 and C4 vertebrae, maturation will be classified as stage CS1, CS2, CS3, CS4, CS5 or CS6. The intra and interexaminer reliabilities will be analyzed using the Kappa’s Concordance Coefficient. On the other hand, correlation between the maturation stage of the palatal suture, the stage of maturation of the cervical vertebrae and age will be performed using the Spearman Correlation Test. The stages of MPSM and CVM distribution was calculated through the relative and absolute frequencies in individuals distributed in different age groups.
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25
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JULIANA CAMPOS PINHEIRO
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BIOLOGICAL EFFECT OF LOW-LEVEL LASER IRRADIATION ON OSTEOBLASTS CULTURED ON THE SURFACE OF 3D POLYMERIC SCAFFOLDS
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Advisor : CARLOS AUGUSTO GALVAO BARBOZA
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COMMITTEE MEMBERS :
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CARLOS AUGUSTO GALVAO BARBOZA
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MARCIA CRISTINA DA COSTA MIGUEL
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PEDRO PAULO DE ANDRADE SANTOS
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CARLOS EDUARDO BEZERRA DE MOURA
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RICARDO LUIZ CAVALCANTI DE ALBUQUERQUE JUNIOR
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Data: Dec 21, 2022
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Show Abstract
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The first part of the work evaluated, through a systematic review of in vitro studies, the applicability of photobiomodulation as an auxiliary tool in tissue engineering. Of 8373 studies initially identified from the search strategies, ten articles met the inclusion criteria for analysis. Data obtained from most of the reviewed studies indicated that low-intensity laser therapy (LLLT) could increase the proliferation and differentiation of cells cultured on the surface of biomaterials. The second part of the work evaluated the effect of LLLT at a dose of 4 J/cm² on the proliferation of osteoblasts (OFCOL II) cultivated on the surface of three-dimensional (3D) polymer scaffolds of polylactic acid (PLA) and PLA associated with chitosan (PLA/Q) produced by the solution blow spinning technique. The Alamar Blue assay demonstrated that OFCOL II cells cultured on 3D PLA scaffolds and irradiated showed more significant proliferative activity when compared to non-irradiated groups within 72 h. Furthermore, OFCOL II cells cultured on PLA/Q scaffolds showed higher proliferative activity at 24 h. Analysis by scanning electron microscopy (SEM) showed that the osteoblasts were anchored in the concavities of the fibers of the examined scaffolds. It was concluded that the proposed model showed potential for studies in the field of bone tissue engineering. The third part of the work evaluated the influence of infrared (IR) and red (R) LLLT at different dosages (1 J/cm², 4 J/cm², and 6 J/cm²) on the proliferation and viability of OFCOL II cells. The Alamar Blue assay showed significant differences (p<0.05) in the mitochondrial activity of group IR using the dose of 1 J/cm² and 4 J/cm² at 24 and 48 h. The Live/Dead assay showed that LLLT induced an increase in cell viability in the IR group at a dose of 4 J/cm² compared to the other groups. Taken together, the results suggest that LLLT can promote in vitro biostimulation of osteoblasts, even when cultivated on the surface of 3D polymeric scaffolds, thus representing a promising tool in bone tissue engineering techniques.
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