Banca de DEFESA: MOISÉS WILLIANS DA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MOISÉS WILLIANS DA SILVA
DATE: 11/07/2025
TIME: 14:00
LOCAL: Auditório do Departamento de Odontologia
TITLE:

Anti-bacterial and antibiofilm potential of Nopalea cochenillifera on microorganisms in the oral environment

KEY WORDS:

Biofilms, Anti-bacterial, Phytotherapy, Nopalea cochenillifera

PAGES: 46
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUBÁREA: Clínica Odontológica
SUMMARY:

The oral environment is a diverse microbiota coexisting in symbiosis. The imbalance of this ecosystem favors the emergence of biofilm-dependent diseases. Therefore, it is necessary to search for complementary natural alternatives that restore moderation. The cactus Nopalea cochenillifera (palm) is abundant in Northeastern Brazil and represents a good source of compounds with biological effects. The objective of the present study is to evaluate the antimicrobial activity of Nopalea cochenillifera (NP) on microorganisms resident in the mouth. The bacteriostatic, bactericidal and antibiofilm action of the hydroethanolic extract of the plant cladodes on Streptococcus oralis (ATCC 10557), S. salivarius (ATCC 7073) and S. mutans (ATCC 700610) was investigated. Each assay was performed in triplicate. The cultures were standardized so that each microbial suspension was adjusted in a spectrophotometer at a wavelength of 625 nm, equivalent to 106 CFU/mL. Subsequently, the bacterial inocula, the extract at different concentrations and Mueller Hinton broth enriched with 15% sucrose were added to 96-well plates. The same procedures were performed with the positive and negative controls, respectively, 0.12% chlorhexidine digluconate, distilled water and the culture medium. The plates were incubated at 37 ± 1 ˚C for 24 hours and the growth, microbial death and antibiofilm action were indicated by the addition of an aqueous solution of resazurin, seeding in petri dishes containing Mueller Hinton Agar and by means of the Elisa reader. It was observed that the NP extract did not present bacteriostatic and bactericidal action at the concentrations proposed in the study; however, it presented antibiofilm activity for all bacterial strains and concentrations tested, especially against S. mutans. The structural diversity of bioactive compounds in plants presents significant opportunities for the discovery of new therapeutic agents, including antibiofilm activity against bacteria that present all the bacterial requirements for cariogenicity.

COMMITTEE MEMBERS:
Presidente - 1958729 - RUTHINEIA DIOGENES ALVES UCHOA LINS
Externa ao Programa - 3469623 - MAURÍLIA RAQUEL DE SOUTO MEDEIROS - UFRNExterno à Instituição - CARLUS ALBERTO OLIVEIRA DOS SANTOS