Banca de DEFESA: LÍLIAN ANDRADE CARLOS DE MENDONÇA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : LÍLIAN ANDRADE CARLOS DE MENDONÇA
DATE: 01/12/2023
TIME: 14:00
LOCAL: Auditorio do PPGBioEF
TITLE:

NEUROPROTECTIVE ACTION OF AYAHUASCA ON THE PREFRONTAL CORTEX OF SAGUIS (Callithrix jacchus) INDUCED INTO A DEPRESSIVE STATE

KEY WORDS:

Major depressive disorder, Marmoset common, Prefrontal cortex, Ayahuasca, Stereology

PAGES: 55
BIG AREA: Ciências Biológicas
AREA: Morfologia
SUMMARY:

Major depressive disorder can present as a single or recurrent episode, lasting days, with different symptoms influencing everyday relationships. One of the main causes of this disorder is chronic stress, and the biological response to these stressors can cause dysregulation in the human response. This diversity in the possible causes of depression leads to other focuses of studies, and our group aims to identify the neuroprotective competence of Ayahuasca on the brain of marmosets (Callithrix jacchus) subjected to an induced chronic stress protocol. The results were evaluated through stereology applied to histological sections. 4 males and 4 females were used, allocated into 3 groups, 02 in the family group (GF), 02 in the isolated group (GI) and 04 in the treated group (GT), all selected randomly. We selected only specimens in juvenile phase I and II equivalent to 7 to 9 months of age. The behavioral record was collected using the continuous focal sampling method, collecting frequency and/or duration of behaviors. A fraction (1/6) of the sections obtained were subjected to histological staining with Nissl techniques, using thionine as dye. To estimate the morphoquantitative parameters, stereology, a three-dimensional histological quantification technique, was used. Among the behaviors, we did not find significant differences between the groups in the experimental phase, except in food intake (H(2) = 6.00, p = 0.050). Regarding the total volume of the prefrontal cortex (PFC), no significant difference was found between groups (H(2) = 3.00, p = 0.223). With regard to neuronal density, it visually appeared to be more intense in GF and GT compared to GI in the three selected areas of the prefrontal cortex. Regarding the mean neuronal volume of the medial prefrontal cortex (VN-mCPF), no significant difference was found between groups (H(2) = 4.00, p = 0.135); in the mean neuronal volume of the dorsolateral prefrontal cortex (VN-dlCPF), no significant difference was found between groups (H(2) = 4.50, p = 0.105); in the mean neuronal volume of the dorsolateral prefrontal cortex (VN-dlCPF), no significant difference was found between groups (H(2) = 4.50, p = 0.105). In this study, we observed a subtle reduction in the volume of the marmoset's prefrontal cortex when comparing the GF with the GI, which can be corroborated by some behaviors, dependent on social command, which also showed a reduction. In the mCPF and dlCPF we observed an average reduction of 25% in neuronal density when comparing the GF with the GI, whereas when comparing the GF with the GT we observed similarity in quantities. The atrophy of neurons in the prefrontal cortex is associated with depressive and anxiety disorders. This characteristic was identified in marmosets from the GI region of the three regions of the PFC (medial, dorsolateral and ventral), suggesting that it is a marker of the depressive state. These results indicate that Ayahuasca provided protection in the total volume of the cortex, as well as in the density and neuronal volume of the marmosets, in relation to the changes caused by the depressive state. Therefore, more studies are needed to understand the underlying mechanisms and determine whether ayahuasca tea has potential as a preventive intervention for depression.

COMMITTEE MEMBERS:
Interno - 4363493 - EXPEDITO SILVA DO NASCIMENTO JUNIOR
Externo à Instituição - FELIPE PORTO FIUZA
Presidente - 1076490 - FERNANDO VAGNER LOBO LADD