Banca de DEFESA: EVERTON LIRA FACANHA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : EVERTON LIRA FACANHA
DATE: 29/06/2023
TIME: 15:00
LOCAL: Videoconferência: https://meet.google.com/rcs-oysz-dpe
TITLE:

 

 EFFECTS OF HYPERBARIC OXYGEN THERAPY ON THE TESTICLES OF RATS WITH DIABETES INDUCED FOR STREPTOZTOCIN

KEY WORDS:
PAGES: 39
BIG AREA: Ciências Biológicas
AREA: Morfologia
SUBÁREA: Histologia
SUMMARY:

Diabetes Mellitus (DM) is characterized as a metabolic and chronic disorder related to problems in the production, secretion and/or use of insulin. There are several complications evidenced in diabetic individuals, from neuropathies, heart diseases, retinopathies, among many OHBers, including infertility. Male individuals living with DM present alterations in their reproductive system, such as disturbances in spermatogenesis, reductions in testosterone levels, increased apoptosis of spermatogonia and spermatocytes, reduction in the number and mobility of spermatozoa and alterations in the distribution of Leydig cells. Among the techniques that can prevent or mitigate the various damages caused by DM in the individual, hyperbaric oxygen therapy (HTO) is considered one of the most promising. In this technique, 100% oxygen at pressures greater than sea level or an absolute atmosphere (ATA) plays an influencing role in oxidative metabolism enabling the reduction mainly of edema and inflammatory processes, offering benefits related to maintenance and/or tissue repair as in the case of wound healing. This study aims to analyze the action of hyperbaric oxygen therapy in preventing testicular morphological changes in diabetic rats induced by streptozotocin. Twenty-eight male Wistar rats (Rattus norvegicus) (60 days old), weighing 220-280g were divided into four groups: C (normoglycemic animals), n=7; C+OHB (normoglycemic animals submitted to HBOT), n= 7; DM (diabetic animals) n= 7; DM+OHB (diabetic animals submitted to HBOT), n=7. DM was induced by streptozotocin (40 mg/kg intraperitoneally) and treatment with HBOT was carried out for six weeks, on continuous days. After treatment the animals were weighed and euthanized and had the testicles collected and weighed. In the collected testes, histological analysis of tissue integrity was performed on slides stained with Hematoxylin and Eosin, as well as morphometric analysis on slides stained with toluidine blue, and immunohistochemistry with immunostaining for testosterone receptors. The treatment was not able to reduce the glycemia of the animals nor did it positively influence the biometric parameters. As for the morphometric parameters, the treatment was not able to mitigate the morphological changes in the testicular tissue, there was no significant differences between the DM and DM+HBOT groups in relation to epithelium height, seminiferous tubule diameter and lumen diameter. In the diabetic groups, there was less availability of testosterone receptors and HBOT treatment possibly did not promote an effect in reducing the loss of these receptors. The results showed us that HBO seems to interfere with the cell cycle of the germ line, causing a possible deleterious effect in relation to the height of the seminiferous epithelium, and consequently the diameter of the lumen.

COMMITTEE MEMBERS:
Interno - 1667882 - BENTO JOAO DA GRACA AZEVEDO ABREU
Externo à Instituição - FLAVIO SANTOS DA SILVA
Presidente - 2477216 - NAISANDRA BEZERRA DA SILVA FARIAS