Banca de DEFESA: ANDRÉ FELIPE MORAIS DE LIMA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ANDRÉ FELIPE MORAIS DE LIMA
DATE: 01/06/2021
TIME: 15:00
LOCAL: Vídeo conferência
TITLE:

STEREOLOGICAL AND HISTOPATHOLOGICAL ANALYSIS OF PROLEY LIVER IN MICE SUBMITTED TO ETHANOL DURING PRE AND POST-CHRISTMAS PERIODS.

KEY WORDS:

Ethanol; Liver; Pregnancy; Lactation; Morphology

PAGES: 70
BIG AREA: Ciências Biológicas
AREA: Morfologia
SUMMARY:

The initial effects of alcohol on the body are well-being and pleasure, however the continued use causes dependence and deleterious effects on organs that are important for life, especially in the female body. We can observe in the literature that the alcohol consumed by women during pregnancy and / or breastfeeding passes into the organism of the offspring, bringing negative effects to the fetal and baby development, being one of the main known teratogenic agents. Excessive and prolonged exposure to alcohol in the body of adult individuals affects liver structure and function causing changes in Alcoholic Liver Disease (ADL). In view of the importance of the liver for the functioning of the organs, it is important to observe and describe whether exposure to alcohol in pregnancy and / or lactation can cause damage to the liver of the offspring, similar to ADL. Thus, the objective of this work is, through histopathological and stereological analyzes, the morphology of the liver of mice generated under the effect of ethanol during pregnancy and / or lactation. For this, 23 young male and female mice (Mus muscullus) were separated into 4 groups: The Control Group (CG) (n=6): offspring from mothers who did not receive alcohol during pregnancy and lactation; Gestation Group (GEtG) (n=5): offspring from mothers who received 15% alcohol only during pregnancy; Lactation group (GEtL) (n=6): offspring from mothers who received 15% alcohol only during lactation; and Gestation + lactation group (GEtGL) (n=6): offspring from mothers who received alcohol during the entire period of pregnancy and lactation. After 34 to 36 days of life, the animals were weighed and euthanized, the liver removed and preserved for further processing and histological analysis. The histopathological diagnosis was obtained through the qualitative description of the tissues and semi-quantitative scores. Stereological analyzes were performed to obtain the volume of the figure using the Cavaliere method. The data obtained were submitted to analysis by ANOVA and post hoc by GABRIEL, with a significance level of 5% (p <0.05). There was a reduction in body weight at weaning of the animals of the GEtL and GEtGL group when compared to the other groups, there was also a decrease in the final body weight and liver weight of the GEtL group when compared to the GEtG. All groups treated with alcohol had tissue damage characteristic of alcoholic liver disease, such as: presence of micro and macro vesicular steatosis, edematous degeneration and areas suggestive of necrosis. According to the semi-quantitative analyzes, the GetGL group showed more liver damage when compared to the other groups. The exposure of alcohol during the lactation phase has negative effects on the nutrition of the offspring, compromising body weight and liver, due to the fact that alcohol intervenes in the production and composition of breast milk, as well as in its consumption. Histopathological changes arise due to the accumulation of reactive oxygen species produced during alcohol metabolism, affecting the structure of hepatocytes. Thus, the exposure of alcohol in an acute form in the stages of pregnancy and / or lactation showed deleterious effects for the offspring, compromising the development and the hepatic morphophysiology.

BANKING MEMBERS:
Presidente - 4507066 - CHRISTINA DA SILVA CAMILLO
Externo à Instituição - FRANCISCO GILBERTO OLIVEIRA - URCA
Externo ao Programa - 2318723 - SERGIO ADRIANE BEZERRA DE MOURA