IDENTIFICATION OF THE PREMAMMILLARY NUCLEI IN ROCK CAVY (Kerodon rupestris): CYTOARCHITECTURE AND IMMUNOHISTOCHEMISTRY AGAINST NOS
Hypothalamus; Ventral premammillary nucleus; Dorsal premammillary nucleus; Nitric oxide synthase.
The ventral premammillary nucleus (PMV) and the dorsal premammillary nucleus (PMD) are in the basomedial hypothalamus, being part of columns that control the reproductive and defensive behaviors, respectively. The rat PMV is a compact group of neurons. Rostrally it is drop-shaped, being more rounded caudally. The PMV has bidirectional connections with nuclei involved with the reception of pheromonal information and sexual behavior. The PMV express sexual hormone receptor and leptin receptor, so it is in a good position to evaluate the reproductive and nutritional status. However, PMV lesion causes decrease in reproductive behavior without change the energy balance. The PMD is dorsocaudal to the PMV. In rat it is square-shaped rostrally and rounded caudally. It has many connections with nuclei involved with defensive behavior. However, functional studies using rats point to a division of the PMD with its ventrolateral part (PMDvl) involved with defensive behavior against predator, and its dorsomedial part (PMDdm) involved with defensive behavior towards a co-specific. The present study evaluates the presence and the cytoarchitecture of these nuclei in the hypothalamus of the rock cavy (Kerodon rupestris), an endemic rodent of the Brazilian Northeast. To that end, we used Nissl staining and immunohistochemistry against the nitric oxide synthase (NOS). Both methods of staining showed the presence of the PMV and PMD in the rock cavy’s hypothalamus with location and morphology very similar to the rat. However, the rock cavy’s PMV appears more rostral and the anti-NOS staining seems to show the PMDvl and PMDdm subdivisions.