Banca de DEFESA: VALÉRIA MILENA DANTAS DE CASTRO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : VALÉRIA MILENA DANTAS DE CASTRO
DATA : 28/02/2019
HORA: 09:00
LOCAL: Auditório do Programa de Pós-graduação em Biologia estrutural e Funcional
TÍTULO:

EFFECTS OF EXTRACT ALLIUM CEPA L AND S-METILCYSTEIN IN MORPHOLOGY OF DUODENE OF RATS WITH DIABETICS INDUCED BY ESTREPTOZOTOCIN.

PALAVRAS-CHAVES:

Diabetes Mellitus type 1, phytotherapics, stereology, cytokines, duodenum.

PÁGINAS: 48
GRANDE ÁREA: Ciências Biológicas
ÁREA: Morfologia
SUBÁREA: Histologia
RESUMO:

Diabetes Mellitus is a worldwide epidemic that is expected to increase in the coming decades. It is a heterogeneous metabolic disorder and is classified as three major forms, DM1, DM2 and gestational diabetes. The experimental DM1 being the focus of this study. DM1 is characterized by being an autoimmune disease, where the immune-mediated destruction of pancreatic β cells occurs, with consequent insulin deficit, leading to hyperglycemia which in turn leads to damage such as retinopathy, neuropathy, gastrointestinal disorders, among others. The objective of this study is to evaluate the alterations due to DM1 in the duodenal portion and the possible attenuating or reversing effects of the extract of Allium cepa L. and its isolated amino acid S-methylcysteine in its morphology. For this purpose 35 male Wistar animals, aged 90 days and weighing between 250 and 300 g, were used. The animals were housed in polypropylene boxes provided with drinking fountain and feeder. They were divided into four groups: C (control), D (diabetic group without treatment), DSM (diabetic group treated with S-methylcysteine) and DAC (diabetic group treated with Allium cepa L extract). The treatment period lasted thirty days, then euthanasia was performed with a lethal dose of isoflurane for sample withdrawal. The samples were then fixed to follow routine histological processing with HE staining to determine the volume by the Cavalieri principle, the proportion occupied by the duodenum layers by the Delesse principle, absolute volume. Blood collection was also performed to analyze the profile of pro-inflammatory cytokines (IL-1B and IL-6) and anti-inflammatory cytokines (IL-10). The treatments used reduced the glycemia in the DSM, DAC groups compared to the D group, but in the loss of weight, polydipsia and polyphagia had no positive effect. As for the increase in the reference volume of wall, lumen and total volume only the DSM group had improvement of the changes. In the absolute volume of mucosa, submucosa and muscle both the extract and the amino acid had a positive effect, the latter being more efficient. In the cytokine analysis, the extract had a non-significant reduction of the proinflammatory cytokines IL-1B and IL-6, while the amino acid reinforced the anti-inflammatory activity of IL-10. From the results, it was concluded that the treatments were able to reduce glycemia, partially reverse stereological changes and act positively on the cytokine profile present in the serum. With this, it can be concluded that phytotherapic intervention can act as a coadjuvant alternative in the treatment of diabetes with benefits both pre-clinical and duodenal tissue level.

MEMBROS DA BANCA:
Externo à Instituição - GILBERTO SANTOS CERQUEIRA - UFC
Externa ao Programa - 1714418 - KARINA CARLA DE PAULA MEDEIROS
Presidente - 2477216 - NAISANDRA BEZERRA DA SILVA FARIAS