Banca de DEFESA: RODRIGO SERAFIM DE ARAUJO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : RODRIGO SERAFIM DE ARAUJO
DATA : 12/12/2016
HORA: 08:30
LOCAL: Sala de Reuniões do Departamento de Morfologia
TÍTULO:

Morphology and epididymal morphometry of the bat Artibeus planirostris (CHIROPTERA: PHYLLOSTOMIDAE)

PALAVRAS-CHAVES:

Reproductive seasonality, bats, epididymis.

PÁGINAS: 3454
GRANDE ÁREA: Ciências Biológicas
ÁREA: Morfologia
SUBÁREA: Histologia
RESUMO:

Although studies on the reproductive biology of bats have intensified in Brazil, for many species and in several regions of the country these information are still scarce, such as is the case of Artibeus planirostris. The objective of this study was to understand the reproductive parameters of this species, from the morphological and morphometric analysis of the epididymis. Sixteen adult animals were collected during the dry season (n = 08) and rainy season (n = 08) in 2014. The captures were taken at dusk on central campus of Federal University of Rio Grande do Norte (Natal-RN, Brazil) (SISBIO authorization no. 25233-1), using mist nets. After anesthesia and euthanasia by transcardiac perfusion with 4% paraformaldehyde, the epididymis were collected (CEUA/UFRN authorization no. 009/2012), followed by histological processing for embedding in historesin and analysis under light microscopy. Morphometric analyzes of the epididymal parenchyma were performed using images from the histological slides, by the Image-Pro Plus. The results were submitted to ANOVA, and the means were compared by the Student-Newman-Keuls test at 5%, followed by the Tukey post-test. At the two analyzed seasons, the morphological evaluation showed that the organ was divided into 4 main regions: initial segment, head, body and tail. The parenchyma was predominantly composed of epididymal tubules, supported by intertubular connective tissue. During the three main epididymal portions, the tubules occupied 75.07 ± 2.94 % of parenchyma in rainy season and 67.23 ± 2.44 % in dry season, and its percentages in the head and body were significantly larger in rainy season than in dry season. The remaining part of parenchyma was represented by the intertubular region, which showed proportional reduction in the same regions and seasons. The tubules were composed of cylindrical pseudostratified epithelium with stereocyllium, supported on a basement membrane and lumen. The epididymal epithelium was the main component of the epididymal tubule. In the tail region, its percentage was higher in dry season (27.58 ± 8.33%) than in rainy season (17.79 ± 4.33%). This epithelium was composed of the main (MA), basal (BA), narrow (NA), halo (HA), clear (CL) and apical (AP) cells. The first and last cells ones showed, respectively, the highest and the lowest distributions in all epididymal regions, in both rainy and dry seasons. In the head and tail regions, MA cells were the most predominant in the epididymal epithelium in the dry season in relation to the rainy season, while BA cells were the most predominant in the rainy season, such in head as in tail. AP cells had a higher distribution in epididymis head during the rainy season, while NA cells had a greater distribution in the epididymis body during dry season. There was a increase in several parameters, from the head to the tail, such as the tubular diameter, luminal diameter and its percentages with spermatozoa, and the muscle cells surrounding the epididymal tubules, in contrast with a decrease in epithelial high. Several of these parameters showed larger values in the rainy season in relation to the dry season. It can be concluded that the epididymis of A. planirostris was similar to that described for other mammals. It was found spermatozoa in the lumen of epididymal tubules during all the seasons, especially in the tail region, showing a continuous annual reproductive pattern, with reproductive peaks in the rainy season.

MEMBROS DA BANCA:
Presidente - 1718086 - DANIELLE BARBOSA MORAIS
Interno - 1330828 - CARLOS EDUARDO BEZERRA DE MOURA
Externo à Instituição - SERGIO LUIS PINTO DA MATTA - UFV