Banca de DEFESA: CAMILLA CARLA DO NASCIMENTO DANTAS CARDOSO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : CAMILLA CARLA DO NASCIMENTO DANTAS CARDOSO
DATA : 12/12/2016
HORA: 13:00
LOCAL: Sala de Reuniões do Departamento de Morfologia
TÍTULO:

Anti-inflammatory potential of microparticles containing Triamcinolone in the experimental ulcerative colitis model.

PALAVRAS-CHAVES:

Triamcinolone, ulcerative colitis, microparticles, histopathology

PÁGINAS: 62
GRANDE ÁREA: Ciências da Saúde
ÁREA: Medicina
SUBÁREA: Anatomia Patológica e Patologia Clínica
RESUMO:

Inflammatory bowel disease (IBD) encompasses a spectrum of chronic inflammatory disorders and is classified into two major subtypes: Ulcerative Colitis (UC) and Chron's Disease (DC). The treatment of UC is based on the use of anti-inflammatories, but these drugs in their conventional form generate numerous adverse effects, which stimulates the development of research focused on the search for new therapies and technologies applied to the drugs. The objective of this work was to evaluate the activity of microparticles coated by chitosan and guar gum biopolymers containing as active principle triamcinolone in order to observe the anti-inflammatory response in the macro and microscopic parameters of the intestinal mucosa, as well as the activity of the disease In response to the experimental model of 4% acetic acid-induced ulcerative colitis in Wistar rats. G2: Colitis (absence of colitis and absence of treatment), G3: Sulfasalazine (SSZ) (colitis + G5: free TR (colitis + 10mg / kg / day drug), G6: free TR (colitis + 5mg / kg / day drug) Kg / day drug), G7: Microparticle of triamcinolone (colitis + 15mg / kg / day drug) and group G8: Biopolymers (Colite + vehicle Chitosan-Gum guar). The experiment lasted eight days and induction of colitis occurred on the third day. The treatments were administered every day of the experiment, except on the 8th day, in which the animals were euthanized for posterior macro and microscopic analysis of the colon. The results obtained for the macroscopic evaluation showed that the lowest degree of injury, except for the negative control group, was found in the Biopolymer group (score 2.7) when compared to the other groups: Colite (score 6.6), SSZ (score 3.2), TR 5mg (score 7.5), TR 10mg (score 4.4), TR 15mg (score 3.3) and TR microparticle (score 3,7). In the statistical analysis of this parameter, the groups treated with SSZ 500mg / kg / day, TR 15mg / kg / day, TR microparticle 15mg / Kg / day and Biopolymers (microparticle vehicle) differed statistically when compared to the Colite group (p < ), This difference being more significant in the Biopolymer group (p <0.01). In the analysis of weight loss, the TR microparticle reduced this parameter by 8 to 12% when compared to the free Triamcinolone groups, differing statistically (p <0.05), suggesting that the technology employed was satisfactory in terms of reducing this adverse effect . In the histopathological evaluation the TR group 15mg / kg / day obtained the lowest tissue damage, evidencing the anti-inflammatory potential of the free drug. In view of the results, it is suggested that nanotechnology with the use of microparticles was able to reduce adverse effects, such as weight loss and, in addition, obtained better results when compared to the free Triamcinolone 10mg group, since there was release of Only 69% of the 15mg of triamcinolone encapsulated in 24 hours. Thus, studies aimed at the application of nanotechnology and new assets should grow and expand in order to seek new therapies and elucidate mechanisms of action that have not yet been discovered.

MEMBROS DA BANCA:
Presidente - 4507066 - CHRISTINA DA SILVA CAMILLO
Externo ao Programa - 2569445 - LOURENA MAFRA VERISSIMO
Externo à Instituição - JEYMESSON RAPHAEL CARDOSO VIEIRA - UFPE