Banca de QUALIFICAÇÃO: MARDEM PORTELA E VASCONCELOS BARRETO
Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.DISCENTE : MARDEM PORTELA E VASCONCELOS BARRETO
DATA : 15/06/2016
HORA: 11:00
LOCAL: Auditório do Departamento de Morfologia
TÍTULO:
EFFECT OF ASSOCIATION OF LASER THERAPY AND PROPOLIS ON WOUND HEALING IN DIABETIC RATS
PALAVRAS-CHAVES:
Diabetes Mellitus; wound healing; lasers; propolis.
PÁGINAS: 42
GRANDE ÁREA: Ciências Biológicas
ÁREA: Morfologia
SUBÁREA: Histologia
RESUMO:
Diabetes mellitus is a disease that causes several systemic complications such as delayed healing process. Several therapies have been tested in in vitro and in vivo studies in order to promote improvement in the wound healing process in diabetic animals and humans. The aim of this study was to evaluate the association of low level laser therapy and topical administration of propolis in a skin wound model in rats with induced diabetes. Diabetes was induced by streptozotocin administration and confirmed after five days by measuring the glycemic index. Surgical wounds were made on the backs of the animals (n = 96), that were divided into four groups: (C) control, no treatment; (L) exposed to low level laser therapy (4 J/cm2 every 24 hours for 6 days); (P) submitted to topical administration of propolis (alcoholic extract at 30%); and (LP) submitted to the combination of laser therapy and topical administration of propolis. Surgical areas were photographed every other day, for evaluation of the wound closure area. At intervals of 7, 14 and 21 days of the animals were euthanatized and the wound area blocks were removed. The specimens were fixed, processed routinely and embedded in paraffin and the obtained sections were stained with H/E, toluidine blue and Picrosirius red for evaluation of re-epithelialization rate, intensity of the inflammatory infiltrate, number of mast cells and collagen formation and organization. Histological sections were also submitted to immunostaining. Quantitative data were submitted to nonparametric tests, considering a confidence interval of 95% (p <0.05). Initial evaluations show that L and LP groups exhibit better results of the repair process parameters compared to the groups P and C. It is expected that the results of this research can support future clinical studies aimed at establishing a more efficient therapy for skin wounds and consequent improvement of life quality in diabetic patients.
MEMBROS DA BANCA:
Externo ao Programa - 1667882 - BENTO JOÃO DA GRAÇA AZEVEDO ABREU
Presidente - 2477216 - NAISANDRA BEZERRA DA SILVA FARIAS
Interno - 2329140 - RAIMUNDO FERNANDES DE ARAUJO JUNIOR