Distribution of the neuronal nitric oxide in the diencephalon of the rock cavy (Kerodon rupestris)
Nitric oxide; Kerodon rupestris; diencephalon; hypothalamus.
Nitric oxide (NO) is a very simple molecule (N=O), gas with chemical properties of a free radical which was considered until the 80s just a member of a family of undesirable environment pollutant with a potential to be a carcinogen. Since its discovery in the nervous system NO has been implicates in several functions, what is in agreement with its wide distribution in the brain. NO was described in the brain of many species but not in the rock cavy (Kerodon rupestris), a rodent endemic from the Brazilian caatinga that lives in rocky areas and has crepuscular habits. All those features make this rodent an interesting animal to study the NO distribution. Using immunohistochemistry against NO synthase (NOS), enzyme that synthesizes the NO, and NADPHdiaphorase histochemistry we could identify indirectly the presence of nitrergic neurons in the diencephalon. In the actual description we evaluated only the hypothalamus and we could see that neurons NOS-immunorreactive (NOS-IR) were very intense inside the supraoptic nucleus and the lateral part of the retrochiasmatic area, both with neurons highly stained. Inside the septohypothalamic nucleus, median preoptic nucleus, lateral preoptic area, medial magnocelullar and ventral parvocelullar divisions of the paraventricular nucleus of the hypothalamus, the tuberal part of the lateral hypothalamic area, the ventrolateral division of the ventromedial nucleus of the hypothalamus, the dorsal premammillary nucleus and the lateral division of the medial mammillary nucleus the density of neurons NOS-IR was high. The density was moderated in the medial division of the medial preoptic nucleus, magnocelullar preoptic nucleus, ventrolateral nucleus of the hypothalamus, peduncular part of the lateral hypothalamic area, dorsomedial division of the ventrolateral nucleus of the hypothalamus, compact and ventral divisions of the dorsomedial nucleus of the hypothalamus, ventral premammillay nucleus and the posterior nucleus of the hypothalamus. Inside the medial preoptic area, anterior parvocelullar division of the paraventricular nucleus of the hypothalamus, periventricular nucleus, anterior division of the ventromedial nucleus of the hypothalamus and dorsal division of the dorsomedial nucleus of the hypothalamus the density was low. The analysis will be extended to the other parts of the diencephalon along with the description of the types of NO-IR cells.