Banca de DEFESA: RAQUEL COSTA SILVA DANTAS KOMATSU

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : RAQUEL COSTA SILVA DANTAS KOMATSU
DATE: 15/12/2023
TIME: 14:00
LOCAL: REMOTA - meet.google.com/mvw-xjrh-vqr
TITLE:

Leukocyte-derived microRNA expression associated with left ventricular systolic dysfunction in post-acute myocardial infarction

KEY WORDS:

miRNA; leukocyte; myocardial infarction; left ventricular systolic dysfunction; heart failure; enrichment analysis

PAGES: 59
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Left ventricular systolic dysfunction (LVEDD) is considered a serious consequence of ST-segment elevation myocardial infarction (STEMI) and finding biomarkers of LVEDD is necessary. Thus, immune cell-derived microRNAs stand out as they mediate complications associated with AMI. Therefore, this study aims to evaluate the association of leukocyte-derived microRNA with the development of post-STEMI LVSd. Post-STEMI patients were grouped as having (LVSd, n=9) or not LVSd (non-LVSd, n=16). The expression of 61 microRNAs was analyzed by RT-qPCR and the differentially expressed microRNAs were identified. Principal Component Analysis evaluated the stratification of groups based on microRNA expression. Predictive variables of LVSd were investigated through logistic regression analysis. A system biology approach was used to explore the regulatory molecular network of the disease and an enrichment analysis was performed. The let-7b-5p (AUC: 0.807; 95% CI: 0.63–0.98; p = 0.013), miR-125a-3p (AUC: 0.800; 95% CI: 0.61–0.99; p = 0.036) and miR-326 (AUC: 0.783; 95% CI: 0.54–1.00; p = 0.028) were upregulated in LVSd (p < 0.05) and discriminated LVSd from non-LVSd. Multivariate logistic regression analysis showed let-7b-5p (OR: 16.00; 95% CI: 1.54-166.05; p = 0.020) and miR-326 (OR: 28.00; 95% CI: 2.42-323.70; p = 0.008) as predictors of LVSd. The enrichment analysis of the targets of these three microRNAs is associated with immunological response, cell-cell adhesion, and cardiac changes. Therefore, the expression of let-7b-5p, miR-326, and miR-125a-3p was associated with post-STEMI LVSd, indicating their potential involvement in the cardiac dysfunction physiopathology and highlighting these microRNAs as possible LVSd biomarkers

COMMITTEE MEMBERS:
Presidente - 2087759 - ANDRE DUCATI LUCHESSI
Externa ao Programa - 1837354 - JULLIANE TAMARA ARAUJO DE MELO CAMPOS - UFRNExterna ao Programa - 1055045 - MARCELA ABBOTT GALVAO URURAHY - nullExterno à Instituição - MARCELO MACEDO ROGERO
Externo à Instituição - MARCOS FERREIRA MINICUCCI - UNESP