Banca de QUALIFICAÇÃO: RAQUEL COSTA SILVA DANTAS KOMATSU

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : RAQUEL COSTA SILVA DANTAS KOMATSU
DATE: 11/08/2024
TIME: 14:30
LOCAL: REMOTA - https://meet.google.com/szj-qjjp-ekf
TITLE:

THE LET-7B-5P, MIR-326, AND MIR-125A-3P ARE ASSOCIATED WITH LEFT VENTRICULAR SYSTOLIC DYSFUNCTION IN POST-MYOCARDIAL INFARCTION

KEY WORDS:

miRNA, PBMC, myocardial infarction, left ventricular systolic dysfunction, heart failure, enrichment analysis

PAGES: 11
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Background: Acute ST-elevation myocardial infarction (STEMI) can lead to adverse cardiac remodeling, resulting in left ventricular systolic dysfunction (LVSd) and heart failure. Epigenetic regulators, such as microRNAs, may be involved in the physiopathology of LVSd.

Objective: This study explored microRNAs in peripheral blood mononuclear cells (PBMC) of post-myocardial infarction patients with LVSd.

Methods: Post-STEMI patients were grouped as having (LVSd, n = 9) or not LVSd (non-LVSd, n = 16). The expression of 61 microRNAs was analyzed in PBMC by RT-qPCR and the differentially expressed microRNAs were identified. Principal Component Analysis stratified the microRNAs based on the development of dysfunction. Predictive variables of LVSd were investigated through logistic regression analysis. A system biology approach was used to explore the

regulatory molecular network of the disease and an enrichment analysis was performed.

Results: The let-7b-5p (AUC: 0.807; 95% CI: 0.63–0.98; p= 0.013), miR-125a-3p (AUC: 0.800; 95% CI: 0.61–0.99; p = 0.036) and miR-326 (AUC: 0.783; 95% CI: 0.54–1.00; p = 0.028) were upregulated in LVSd (p < 0.05) and discriminated LVSd from non-LVSd. Multivariate logistic regression analysis showed let-7b-5p (OR: 16.00; 95% CI: 1.54–166.05; p = 0.020) and miR-326 (OR: 28.00; 95% CI:

2.42–323.70; p = 0.008) as predictors of LVSd. The enrichment analysis revealed association of the targets of these three microRNAs with immunological response, cell-cell adhesion, and cardiac changes.

Conclusion: LVSd alters the expression of let-7b-5p, miR-326, andmiR-125a-3p in PBMC from post-STEMI, indicating their potential involvement in the cardiac dysfunction physiopathology and highlighting these microRNAs as possible LVSd biomarkers.

COMMITTEE MEMBERS:
Externo à Instituição - ALVARO DANILO CERDA MAUREIRA - UFRO
Presidente - 2087759 - ANDRE DUCATI LUCHESSI
Externo à Instituição - ERNESTO VARGAS MENDEZ
Externo ao Programa - 1544647 - MATHEUS DE FREITAS FERNANDES PEDROSA - null