Banca de DEFESA: DANIELE CAVALCANTE GOMES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : DANIELE CAVALCANTE GOMES
DATE: 09/08/2024
TIME: 09:00
LOCAL: REMOTA - https://meet.google.com/rus-qzjk-apu
TITLE:

HYBRID NANOCARRIERS WITH A DOUBLE DRUG STRATEGY FOR THE TREATMENT OF INFECTION BY Trypanosoma cruzi

KEY WORDS:

chagas disease; benznidazole; phytol; hybrid nanocarrier; with-nanoencapsulation of assets.

PAGES: 97
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Chagas disease is a neglected tropical disease caused by the protozoan Trypanosoma cruzi. In this scenario, the only drug of choice for treatment in Brazil is Benznidazole (BNZ). Despite its widespread use, this molecule has efficacy problems due to its high toxicity, difficulty in crossing biological barriers, and low solubility in aqueous media. Therefore, the co-delivery of benznidazole together with phytol (FIT), an acyclic monounsaturated diterpene alcohol that expresses promising antitrypanosomal activity, is justified. Nanocarriers have proven ability to cross most biological barriers and intracellular targeting of drugs. The objective of this work was to develop biodegradable hybrid nanocarriers containing benznidazole and phytol, to improve the targeting of the compounds to cells infected by Trypanosoma cruzi. The systems were produced by the nanoprecipitation method. The formulation development and obtaining parameters were optimized by measurements of mean particle size, polydispersity index, zeta potential, atomic force microscopy (AFM), scanning electron microscopy (SEM), infrared absorption spectroscopy with transform Fourier (FTIR-ATR), encapsulation efficiency and in vitro studies. Spherical and stable hybrid nanocarriers below 200 nm were obtained, with encapsulation efficiency greater than 95% for FIT and 30% for BNZ. The in vitro drug release kinetics demonstrated a slow release of the nanocarriers. In vitro assays in normal cells (3T3) and (RAW) showed cytocompatibility and in trypomastigotes (CL-Brenner) showed an increase in the antichagasic activity of nanocarriers containing BNZ-FIT over monoloaded systems and BNZ and free FIT. Thus, the present work systematically addresses the development of a nanotechnological system with innovative potential for increasing the antichagasic response from the association of actives.

COMMITTEE MEMBERS:
Externo à Instituição - ALVARO FEDERICO JIMENEZ KAIRUZ - UNC/AR
Interno - 2087759 - ANDRE DUCATI LUCHESSI
Presidente - 1639820 - ARNOBIO ANTONIO DA SILVA JUNIOR
Externa à Instituição - DANIELE RIBEIRO DE ARAUJO - UFABC