Banca de DEFESA: KEYLLA DAYANNE COELHO MARINHO DE MELO
Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.STUDENT : KEYLLA DAYANNE COELHO MARINHO DE MELO
DATE: 15/09/2023
TIME: 09:00
LOCAL: REMOTA - https://meet.google.com/tun-jjyo-uja
TITLE:
ANTIOXIDANT ACTIVITY OF FUCOIDAN MODIFIED WITH GALLIC ACID USING THE REDOX METHOD
KEY WORDS:
sulfated polysaccharides; oxidative damage; sulfated fucan; antioxidant activity
PAGES: 60
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:
Antioxidant compounds decrease the amount of intracellular reactive oxygen species (ROS) and, consequently, reduce the deleterious effects of ROS in osteoblasts. Here, we modified a 21 kDa fucoidan (FucA) with gallic acid (GA) using the redox method, to potentiate its antioxidant/protective capacity on pre-osteoblast-like cells (MC3T3) against oxidative stress. The 20 kDa FucA-GA contains 37 ± 3.0 mg GA per gram of FucA. FucA-GA was the most efficient antioxidant agent in terms of total antioxidant capacity (2.5 times), reducing power (five times), copper chelation (three times), and superoxide radical scavenging (2 times). Exposure of MC3T3 cells to H2O2 increased ROS levels and activated caspase-3 along with caspase-9. In addition, the cell viability decreased approximately 80%. FucA-GA also provided the most effective protection against oxidative damage caused by H2O2. Treatment with FucA-GA (1.0 mg/mL) increased cell viability (~80%) and decreased intracellular ROS (100%) and caspase activation (~80%). In addition, Fuc-GA (0.1 mg/mL) abolished H2O2-induced oxidative stress in zebra fish embryos. Overall, FucA-GA protected MC3T3 cells from oxidative stress and could represent a possible adjuvant for the treatment of bone fragility by counteracting oxidative phenomena
COMMITTEE MEMBERS:
Presidente - 2195251 - HUGO ALEXANDRE DE OLIVEIRA ROCHA
Externo à Instituição - MOACIR FRANCO DE OLIVEIRA - UFERSA
Externa ao Programa - 2477216 - NAISANDRA BEZERRA DA SILVA FARIAS - nullExterna à Instituição - VALQUIRIA PEREIRA DE MEDEIROS - UFJF
Externa ao Programa - 1720860 - VANESSA DE PAULA SOARES RACHETTI - null