Banca de DEFESA: MAÍRA MARIA DE MENEZES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : MAÍRA MARIA DE MENEZES
DATA : 23/03/2019
HORA: 14:30
LOCAL: Sala Carl Peter von Dietrich
TÍTULO:

A low molecular weight galactofucan from seaweed Spatoglossum schröederi binds fibronectin and inhibits capillary-like tube formation in vitro.

PALAVRAS-CHAVES:

Fucoidan; brown seaweed, endothelial cells; anti-angiogenic

PÁGINAS: 68
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

A low molecular weight (LMW) heterofucan (named fucan B) was obtained from the brown seaweed Spatoglossum schröederi and its activity as inhibitor on endothelial cell (EC) capillary-like tube formation was analyzed. Chemical, infrared and electrophoresis analysis confirmed the fucan B identity. In contrast to other LMW fucans, the fucan B (from 0.012 to 0.1 mg/mL) was able to inhibit EC capillary-like tube formation in a dose-dependent way. In addition, fucan B (from 0.01 to 0.05 mg/mL) did not affect EC proliferation. Fucan B also inhibits EC migration when fibronectin was substrate, but fail when laminin or collagen was substrate. This fucan was biotinylated and used as a probe to identify its action sites. When biotinylated fucan was assayed in confocal experiments, it did not show binding to the cell surface, but rather only to fibronectin. We suggested that fucan B inhibits EC capillary-like tube formation and EC migration because binds directly to fibronectin, and blocking fibronectin sites that are recognized by cell surface ligands. Our data indicate that further studies are needed to evaluate the in vivo effect of fucan B.  

MEMBROS DA BANCA:
Externo à Instituição - DEBORAH YARA ALVES CURSINO DOS SANTOS - USP
Externo ao Programa - 2524706 - GIULIANNA PAIVA VIANA DE ANDRADE SOUZA
Presidente - 2195251 - HUGO ALEXANDRE DE OLIVEIRA ROCHA