Banca de QUALIFICAÇÃO: DANIELE DE SOUZA MARINHO DO NASCIMENTO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : DANIELE DE SOUZA MARINHO DO NASCIMENTO
DATA : 02/12/2016
HORA: 09:00
LOCAL: Sala Carl Peter Von Dietrich - Departamento de Bioquimica
TÍTULO:

THE ANTI-OBESITY PLANT BACCHARIS TRIMERA (LESS.) DC SHOWS ANTIADIPOGENIC EFFECT BY INHIBITING THE EXPRESSION OF PROTEINS INVOLVED IN ADIPOCYTE DIFFERENTIATION.

PALAVRAS-CHAVES:

Baccharis trimera, Adipogenesis, Antioxidant, adipogenic transcriptional factors, chlorogenic acid

PÁGINAS: 19
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
RESUMO:

Baccharis trimera (Less.) DC (gorse) is popularly used to produce a tonic that is consumed against obesity. In this study, we obtained three Baccharis extracts, aquous (AE), decoction (AE-D) and methanol (ME) and their antioxidant in six different tests and their anti-adipogenic effect in 3T3-L1 cells were investigated. The extracts showed antioxidant activity in all tests in dose-dependent way. AE was the most potent in copper and ferric ions chelation assays whereas AE-D was most potent in superoxide and hydroxyl radical scavenging assay, reducing power assay, and total antioxidant capacity. Only ME showed cytotoxic effect against 3T3-L1 cells. Lipid accumulation decreased in 3T3-L1 adipocytes in the presence of extracts (from 0.5 to 1.0 mg/mL). In addition, they attenuated dramatically the levels of adipogenic transcriptional factors, C/EBPα, C/EBPβ and PPARγ, during adipogenesis. AE-D (1.0 mg/mL) highlighting that caused ~90% reduction of the levels of these molecules. Chemical analysis revealed chlorogenic acid the main anti-adipogenic active compound in AE-D and AE. We propose that B. trimera has anti-adipogenic effect and we provided scientific evidences for its efficacy in the development for functional foods.

MEMBROS DA BANCA:
Externo ao Programa - 2578619 - ANA HELONEIDA DE ARAUJO MORAIS
Externo à Instituição - ARIANE FERREIRA LACERDA - UFRN
Presidente - 2195251 - HUGO ALEXANDRE DE OLIVEIRA ROCHA