Banca de QUALIFICAÇÃO: PABLO FELIPE FERREIRA FARIAS

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : PABLO FELIPE FERREIRA FARIAS
DATE: 29/04/2024
TIME: 14:30
LOCAL: Videoconferência - Link para acesso: https://meet.google.com/hqy-tmgw-pbw
TITLE:

EVALUATION OF THE NEUROPROTECTIVE POTENTIAL OF SULFATED POLYSACCHARIDES-RICH SAMPLES FROM CAULERPA CUPRESSOIDES VAR. FLABELLATA

KEY WORDS:

Green seaweed; Biomolecules; Antioxidant; Neuroprotection.

PAGES: 64
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:

Neurodegenerative diseases (NDs), such as Alzheimer's and Parkinson's, are a global health issue affecting millions of people. Oxidative stress is believed to be a key factor in the cause and/or progression of NDs, damaging neuronal cells and leading to symptoms such as memory loss and motor problems. Therefore, the search for new antioxidant agents to mitigate those damages is crucial. Among the potential antioxidant agents, sulfated polysaccharides (PS) obtained from seaweeds stand out. Beyond their antioxidant properties, PS also exhibit various bioactivities properties, including anticoagulant, antiproliferative, and anti-inflammatory effects. Thus, this study evaluated the antioxidant potential of PS-rich fractions from the green seaweed Caulerpa cupressoides var. flabellata against H2O2 in murine neuroblastoma cells (Neuro-2A). Thus, four SP-rich samples (F0.3, F0.5, F1.0, and F2.0) were obtained from polysaccharide-crude extract of Caulerpa cupressoides (PCE). The cytotoxicity and protective effect of the samples against H2O2 in Neuro-2A cells were evaluated, using the 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction method. No cytotoxic effects were observed, except for the PCE and F0.5 at higher concentrations. The F2.0 was the only fraction that showed a potential antioxidant and protection effects against H2O2 under the tested conditions. Additionally, F2.0 reduced the levels of reactive oxygen species (ROS), evaluated using the 2,7-dichlorofluorescein diacetate (DCFH-DA) fluorescent probe. Furthermore, embryotoxicity and optomoral response were evaluated using the Zebrafish model (in vivo). Thus, F2.0 showed no embryotoxic or neurotoxic effects in the Zebrafish embryos at different concentrations, since no changes in mortality, malformations, and optomotor response were observed in the larvae. Therefore, it is expected that the results of this study may contribute to the development of a safe treatment for NDs.

COMMITTEE MEMBERS:
Interno - 2962496 - RAFAEL BARROS GOMES DA CAMARA
Externa ao Programa - 1251018 - RIVA DE PAULA OLIVEIRA - nullPresidente - 1199127 - SILVIA REGINA BATISTUZZO DE MEDEIROS