Banca de DEFESA: ISIANNE KELLY MOURA CERQUEIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ISIANNE KELLY MOURA CERQUEIRA
DATE: 20/10/2023
TIME: 14:00
LOCAL: DEPARTAMENTO DE ODONTOLOGIA - AUDITÓRIO
TITLE:

ANTIMICROBIAL EVALUATION OF A LOW VISCOSITY BULK FILL RESIN WITH XYLITOL INSERTION

KEY WORDS:

Composite resins. Xylitol. Antimicrobial action.

PAGES: 25
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUMMARY:

Introduction: Bulk Fill resins are in good demand among professionals, as their use reduces clinical time and improves the quality of restorations, but they do not have antibacterial activity, which is one of the factors that still hurt people's lives. Biomodification with xylitol aims to improve its clinical characteristics. Objective: To evaluate the antimicrobial action of a Bulk Fill flow resin after inserting xylitol. Methodology: Tetric® N-Flow Bulk Fill resin was mixed with xylitol concentrations (0% [Control], 2.5% and 5% w/w). Cylindrical samples (n=5 from the control group and n=3 from the other groups) were made in acrylic molds with a diameter of 2 mm thick, light-cured for 10 s and stored at 37º C for 24 h. The specimens were sterilized by ultraviolet light for 20 minutes before being placed in a sterile 48-well plate, with 0.5 mL of Mueller Hinton broth added to each well. The plate was incubated at 37 ± 1 ˚C for 48 hours. After the incubation period, the specimens were gently removed, and microbial growth was indicated by adding 100 μL of 0.01% resazurin (SigmaAldrich) aqueous solution with subsequent incubation at 37 ± 1 ˚C for two hours. Viable microorganisms reduce the dye, changing its color from blue to pink. The MIC was defined as the lowest concentration of the substance that inhibited the color change of resazurin. In 0.12%, chlorhexidine was added to a well containing the control group to compare the results generated from the test groups. Result: There was no inhibition of bacterial growth in the wells with inocula containing S. mutans and the resin specimen with xylitol added. Conclusion: This study showed that adding 2.5% and 5% Xylitol to the Tetric® N-Flow Bulk Fill resin did not inhibit bacterial growth.

COMMITTEE MEMBERS:
Presidente - 2042290 - BONIEK CASTILLO DUTRA BORGES
Interna - 2292784 - ISAUREMI VIEIRA DE ASSUNCAO
Externa à Instituição - MARIA ALICE PIMENTEL FUSCELLA