Banca de DEFESA: ANA BEATRIZ COSTA ALMEIDA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ANA BEATRIZ COSTA ALMEIDA
DATE: 31/07/2023
TIME: 08:30
LOCAL: Auditório do Departamento de Odontologia
TITLE:

EVALUATION OF THE ANTI-INFLAMMATORY EFFECT OF SPONDIAS MOMBIN L. EXTRACT IN AN EXPERIMENTAL MODEL OF PERIODONTITIS

 

 

 

 

 

KEY WORDS:

Phytotherapy; Periodontal disease; Spondias.

PAGES: 69
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUBÁREA: Periodontia
SUMMARY:

Periodontitis is a chronic, multifactorial inflammatory disease that affects protective tissues and acquired supports. The hydroethanolic extract of mombin L. spondias (EHSM) has been highlighted due to its antimicrobial action against oral pathogens and its anti-inflammatory and antioxidant activities. The aim of this work was to evaluate the anti-inflammatory effect of EHSM in an experimental model of periodontitis. A pre-clinical, controlled and in vivo test was carried out, using 61 male Wistar rats, randomly distributed in the following groups: saline (n=7); SD (n=14); DP + SM 50 mg/ml (n=12); DP + SM 100mg/kg (n=13) and DP + SM 200 mg/kg (n=15). Gavage was performed daily from the day of periodontitis induction to the 11th experimental day (euthanasia). Blood, gingival and jaw samples were transferred and sent for biochemical, quantitative cytokine (IL-1β and IL-6), histological and computerized microtomographic analyses. For statistical analysis, the parametric ANOVA test was used, followed by Student's t test and the non-parametric Kruskall-Wallis test. The administration of EHSM extract did not cause systemic diseases in the animals, being able to reduce the concentration of IL-6, at a dose of 50mg/kg (p<0.05), and of IL-1β, at a dose of 100mg /kg (p<0.01), as well as improved bone parameters in animals that received EHSM at concentrations of 100mg/kg (p<0.05) and 200mg/kg (p<0.001). In addition, the animals admitted to oral gavage with the extract at concentrations of 100mg/kg (p<0.05) and 200mg/kg (p<0.01) had better histological scores. In conclusion, the findings of this study indicate that EHSM, at different concentrations, presents local anti-inflammatory activity in an experimental in vivo model of periodontitis, without causing any systemic involvement, thus pointing to the potential use of the aforementioned extract in the treatment of this disease.

COMMITTEE MEMBERS:
Presidente - 1958729 - RUTHINEIA DIOGENES ALVES UCHOA LINS
Interno - 1660087 - BRUNO CESAR DE VASCONCELOS GURGEL
Externa à Instituição - Ketsia Bezerra Medeiros - UNI-RN