Banca de DEFESA: DENNYS RAMON DE MELO FERNANDES ALMEIDA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : DENNYS RAMON DE MELO FERNANDES ALMEIDA
DATE: 03/03/2023
TIME: 14:00
LOCAL: PLATAFORMA REMOTA
TITLE:
INFLUENCE OF THE HIPPO PATHWAY IN THE REPAIR OF CHEMICALLY INDUCED ORAL MUCOSITIS IN THE MURINE MODEL SUBJECTED TO LASER TREATMENT, ROYAL JELLY OR PROPOLIS.


KEY WORDS:

Oral mucositis. Phytotherapy. Laser. Via Hippo


PAGES: 55
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUMMARY:
The hippo pathway is a highly conserved signal transduction pathway that is implicated in cell/tissue development, homeostasis and regeneration. YAP plays a key role in the hippo pathway since, together with TAZ, they activate transcription factors that lead to cell growth, differentiation and migration. The YAP/TAZ phosphorylation mechanism by LATS1/LATS2 creates a binding site to keep YAP in the cytoplasm (phosphorylated) preventing its functions at the nuclear level. Given the important functions of this pathway in tissue repair and growth, this research evaluated the immunostaining profile of YAP and LATS2 proteins in oral mucositis (OM) chemically induced by 5-fluororacil (5-FU), in a murine model, treated with propolis ( P), royal jelly (GR) and laser (FBM) compared to the control group (C) without treatment. Samples of male wistar rats divided into the following groups were used: control (C), propolis (P), royal jelly (GR) and laser (FBM) (intraoral 6 J/cm2) separated into three experimental times: days 08, 10 and 14. In the YAP immunostaining analysis, on day 08, the control group obtained scores 0 and 1 in most samples, while on days 10 and 14 most samples obtained scores 2 and 3. In the experimental groups (P, GR, and FBM), score 2 prevailed at all experimental times. For LATS2 there was a prevalence of score 2 both in the control and experimental groups. Regarding the statistical analysis of the immunoexpression of the YAP protein, there was a statistically significant difference (p= 0.020), only on day 08 between the control group compared to the experimental groups (P, GR and FBM). The for LATS2, no statistical difference was found. In the statistical evaluation of the different experimental times within the same group, a statistically significant difference was only found in the laser group and only for LATS2 (p=0.025). Additionally, the spearman correlation was performed between YAP and LATS2 for all groups, but there was no statistically significant association. The greater immunoexpression of YAP and LATS2 (scores 2 and 3) observed the experimental groups indicates that the hippo pathway is activated and seems to influence the repair process in chemically induced oral mucositis treated by different methods.

COMMITTEE MEMBERS:
Interno - 2220417 - CARLOS AUGUSTO GALVAO BARBOZA
Interna - 2492713 - ERICKA JANINE DANTAS DA SILVEIRA
Externa à Instituição - GLÓRIA MARIA DE FRANÇA - UFAL
Externo à Instituição - LUIZ ARTHUR BARBOSA DA SILVA - F.M.Nassau
Presidente - 1298808 - MARCIA CRISTINA DA COSTA MIGUEL
Notícia cadastrada em: 17/02/2023 09:32
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