Banca de DEFESA: JULIANA CAMPOS PINHEIRO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : JULIANA CAMPOS PINHEIRO
DATE: 26/12/2022
TIME: 14:00
LOCAL: Plataforma Google Meet
TITLE:

BIOLOGICAL EFFECT OF LOW-LEVEL LASER IRRADIATION ON OSTEOBLASTS CULTURED ON THE SURFACE OF 3D POLYMERIC SCAFFOLDS

KEY WORDS:

Laser Therapy; Osteoblasts; Cell Proliferation; Biomaterials; Tissue Engineering.

PAGES: 141
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUMMARY:

The first part of the work evaluated, through a systematic review of in vitro studies, the applicability of photobiomodulation as an auxiliary tool in tissue engineering. Of 8373 studies initially identified from the search strategies, ten articles met the inclusion criteria for analysis. Data obtained from most of the reviewed studies indicated that low-intensity laser therapy (LLLT) could increase the proliferation and differentiation of cells cultured on the surface of biomaterials. The second part of the work evaluated the effect of LLLT at a dose of 4 J/cm² on the proliferation of osteoblasts (OFCOL II) cultivated on the surface of three-dimensional (3D) polymer scaffolds of polylactic acid (PLA) and PLA associated with chitosan (PLA/Q) produced by the solution blow spinning technique. The Alamar Blue assay demonstrated that OFCOL II cells cultured on 3D PLA scaffolds and irradiated showed more significant proliferative activity when compared to non-irradiated groups within 72 h. Furthermore, OFCOL II cells cultured on PLA/Q scaffolds showed higher proliferative activity at 24 h. Analysis by scanning electron microscopy (SEM) showed that the osteoblasts were anchored in the concavities of the fibers of the examined scaffolds. It was concluded that the proposed model showed potential for studies in the field of bone tissue engineering. The third part of the work evaluated the influence of infrared (IR) and red (R) LLLT at different dosages (1 J/cm², 4 J/cm², and 6 J/cm²) on the proliferation and viability of OFCOL II cells. The Alamar Blue assay showed significant differences (p<0.05) in the mitochondrial activity of group IR using the dose of 1 J/cm² and 4 J/cm² at 24 and 48 h. The Live/Dead assay showed that LLLT induced an increase in cell viability in the IR group at a dose of 4 J/cm² compared to the other groups. Taken together, the results suggest that LLLT can promote in vitro biostimulation of osteoblasts, even when cultivated on the surface of 3D polymeric scaffolds, thus representing a promising tool in bone tissue engineering techniques.

COMMITTEE MEMBERS:
Presidente - 2220417 - CARLOS AUGUSTO GALVAO BARBOZA
Interna - 1298808 - MARCIA CRISTINA DA COSTA MIGUEL
Interno - 2859541 - PEDRO PAULO DE ANDRADE SANTOS
Externo à Instituição - CARLOS EDUARDO BEZERRA DE MOURA - UFERSA
Externo à Instituição - RICARDO LUIZ CAVALCANTI DE ALBUQUERQUE JUNIOR - UFSC