Banca de QUALIFICAÇÃO: VLADIMIR GALDINO SABINO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : VLADIMIR GALDINO SABINO
DATE: 16/11/2022
TIME: 16:30
LOCAL: Plataforma Google Meet
TITLE:

EFFECTS OF LOW-LEVEL LASER IRRADIATION ON OSTEOBLASTS CULTURED ON NANOFIBROUS POLYLACTIC ACID SCAFFOLDS

KEY WORDS:

photobiomodulation; bone; bone tissue engineering; polymers.

PAGES: 41
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUMMARY:

Bone tissue engineering is an essential branch of regenerative medicine for the repair of defects, malformations, and bone reconstruction, whose development depends on the study of suitable biomaterials and scaffolds with favorable architecture, as well as methods capable of promoting cellular activity, either through growth factors or biophysical stimuli. This study will evaluate the use of low-level laser irradiation (LLLI) to promote in vitro biostimulation of osteoblastic cells cultured on nanofibrous polylactic acid scaffolds. The scaffolds will be produced by the electrospinning technique and characterized in terms of hydrophilicity determined by the sessile droplet contact angle, composition by Fourier transform infrared spectroscopy (FTIR), surface morphology by scanning electron microscopy (SEM) and nanotopography by atomic force microscopy (AFM). The biological assays will be conducted with osteoblasts of the OFCOL II lineage, which will be cultured on the surface of the scaffolds and subjected or not (control group) to irradiation with InGaAIP diode laser at doses of 1, 4, and 6 J/cm², power of 30 mW, using red and infrared light wavelengths. After irradiation, the effects of LLLT on osteoblast proliferation will be evaluated using the Alamar Blue biochemical assay at intervals of 24, 48, and 72h, and cell viability and morphology will be analyzed at 72h, using the Live/Dead assay and scanning electron microscopy (SEM), respectively. The data obtained will be tabulated, and the statistical evaluations will be performed in the GraphPad Prism 6.0 software, adopting a significance level of 5%. It is expected that photobiomodulation will positively influence the viability and proliferation of osteoblasts on the surface of scaffolds, contributing to the development of promising techniques for bone tissue engineering.

COMMITTEE MEMBERS:
Presidente - 2220417 - CARLOS AUGUSTO GALVAO BARBOZA
Interna - 1298808 - MARCIA CRISTINA DA COSTA MIGUEL
Externo à Instituição - CARLOS EDUARDO BEZERRA DE MOURA - UFERSA