Banca de DEFESA: CAIO CÉSAR DA SILVA BARROS

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : CAIO CÉSAR DA SILVA BARROS
DATE: 01/11/2022
TIME: 14:00
LOCAL: PLATAFORMA REMOTA
TITLE:

Study of cell cannibalism and epigenetic modification of histone H3 in giant cell lesions

KEY WORDS:

Giant cell; Giant cell granuloma; Histones; Histone acetyltransferases; Cell-in-cell formation. 

PAGES: 139
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUMMARY:

Background: Central giant cell granuloma (CGCG) of the jaws is a benign lesion that exhibits varied clinical behavior, being classified as non-aggressive or aggressive. This research aimed to morphologically evaluate the cell cannibalism and the immunohistochemical expression of the acetylation of histone H3 lysine 9 (H3K9ac) and ING5 in 19 cases of peripheral giant cell granuloma (PGCG), 38 cases of CGCG (19 non-aggressive cases and 19 aggressive cases) and in 19 cases of giant cell tumor (GCT) of bone, as well as to analyze the association of cell cannibalism and this immunoexpression with the clinical behavior of these lesions. Methods: Cell cannibalism analysis was performed through the quantification of cannibal multinucleated giant cells (CMGC), while the analysis of H3K9ac and ING5 immunoexpression was performed quantitatively and semiquantitatively, respectively, in mononuclear cells and quantitatively in multinucleated giant cells (MGC) and CMGC. Data analysis was performed using Student's t-test and Spearman's rank correlation coefficient. Results: A significant great amount of CGMC was observed in CGCG aggressive compared to non-aggressive CGCG (p = 0.044). There were no significant differences in the CMGC index between PGCG and non-aggressive CGCG (p = 0.858) and between aggressive CGCG and GCT of bone (p = 0.069). CGCG that exhibited rapid growth and tooth displacement and/or root resorption showed a great amount of CMGC (p = 0.035; p = 0.041, respectively). Aggressive CGCG showed higher immunoexpression of H3K9ac (p < 0.0001) and ING5 in MGC and CMGC (p < 0.05; p < 0.0001, respectively) when compared to non-aggressive CGCG. There was no difference in H3K9ac and ING5 immunoexpression between aggressive CGCG and GCT of bone (p > 0.05). A higher frequency of score 4 of ING5 was observed in mononuclear cells in all lesions. H3K9ac and ING5 immunoexpression were associated with aggressive characteristics in CGCG (p < 0.05). Conclusions: Aggressive CGCG shows a high CMGC index when compared to non-aggressive CGCC. Thus, its quantification can help to predict CGCG clinical behavior. The significantly higher H3K9ac and ING5 immunoexpression may reflect greater clastic activity and cell cannibalism induction in aggressive CGCG and, consequently, be associated with greater aggressiveness in these lesions.

COMMITTEE MEMBERS:
Externo à Instituição - CRISTIANE HELENA SQUARIZE - UM
Presidente - 2492713 - ERICKA JANINE DANTAS DA SILVEIRA
Externa à Instituição - KARUZA MARIA ALVES PEREIRA - UFC
Interna - 1258693 - LELIA MARIA GUEDES QUEIROZ
Interna - 1298808 - MARCIA CRISTINA DA COSTA MIGUEL