Banca de QUALIFICAÇÃO: RODOLFO XAVIER DE SOUSA LIMA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : RODOLFO XAVIER DE SOUSA LIMA
DATE: 09/09/2022
TIME: 10:00
LOCAL: DEPARTAMENTO DE ODONTOLOGIA - SALA DE AULAS II
TITLE:

ANALYSIS OF ADHESION, BACTERICIDAL PROPERTIES, AND RELEASE OF AN ANTIMICROBIAL AGENT IN A PIT AND FISSURE SEALANT ASSOCIATED WITH SCHINOPSIS BRASILIENSIS (BRAÚNA)EXTRACT.

KEY WORDS:

pit and fissure sealants; phytotherapy; dental caries.

PAGES: 30
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUBÁREA: Materiais Odontológicos
SUMMARY:

Introduction: dental caries is a chronic oral disease that affects several populations around the world and occurs from the correlation between acidogenic bacteria, fermentable carbohydrates, and dental structure in a dynamic process that can be paralyzed, reactivated, and even reversed through sealing the cavities isthmus. Objectives: the aim of the study it evaluates the effectiveness of the bactericidal properties of the Fluroshield® sealant enriched with the Schinopsis brasiliensis (Braúna) stem extract, as well as the potential for releasing the active ingredient and the bond strength of the experimental materials. Methodology: it will be an “in vitro” experimental study. The Braúna bark will be dried at 40 ± 1 °C and the ethanolic extract will be obtained by percolation of Schinopsis brasiliensis bark powder. The commercial sealant Fluroshield (Dentsply) will be enriched with the proportions by mass (0%
(Control), 20%, 10%, 5%, 2.5% and 1.25%). The microbiological tests will be the Minimum Inhibitory Concentration (MIC) and Inhibition of Biofilm Formation. The release of the active ingredient (gallic acid) will be evaluated by Ultra High-Performance Liquid Chromatography (UHPLC) and the bond strength to dental tissue will be evaluated by microshear. Data will be presented as means + standard error of the mean. The final values of the samples of each test will be submitted to the normality test
( Kolmogorov - Smirnov test) and then submitted to analysis of variance (ANOVA) for the parametric data, followed by the Tukey test (p < 0.05 ). For non-parametric data, the Kruskal Wallis test will be used using the GraphPad Prism 7 software. Expected results: it is expected that the addition of Schinopsis brasiliensis extract will be able to inhibit biofilm formation and that the MIC will be between the groups tested without affecting the ability to release the active ingredient in an aqueous medium and the bond strength of the material to the tooth.

COMMITTEE MEMBERS:
Presidente - 2042290 - BONIEK CASTILLO DUTRA BORGES
Interna - 2292784 - ISAUREMI VIEIRA DE ASSUNCAO
Externa ao Programa - 3138832 - MARIA REGINA MACEDO COSTA - UFRN