Banca de DEFESA: FRANCISCO LEONARDO DA SILVA JÚNIOR

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : FRANCISCO LEONARDO DA SILVA JÚNIOR
DATE: 20/08/2022
TIME: 08:30
LOCAL: DEPARTAMENTO DE ODONTOLOGIA - AUDITÓRIO
TITLE:

EVALUATION OF THE USE OF THE PROBIOTIC LACTOBACILUS CASEI ADJUNCT TO PERIODONTAL THERAPY IN EXPERIMENTAL MODEL OF PERIODONTITIS: A RELATIONSHIP BETWEEN PERIODONTAL AND INTESTINE

KEY WORDS:

Periodontitis; Probiotics; Intestinal dysbiosis; Lactobacillus casei

PAGES: 88
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUBÁREA: Periodontia
SUMMARY:

Periodontitis is a chronic inflammatory condition that can influence the gut microbiota. The gold standard treatment for periodontitis includes scaling and crown-root planing, but in complex cases adjuvant therapies such as probiotics can be used. The use of this adjuvant treatment may contribute to the improvement of periodontal condition and intestinal symbiosis. The aim of this study was to evaluate the effect on periodontal and intestinal inflammation of the use of Lactobacillus casei (LC) adjunct to scaling and root planing (RACR) in Balb/c mice with ligature-induced periodontitis. This study is a preclinical, in vivo, randomized, blinded, placebo-controlled trial consisting of 40 male Balb/c mice. The animals were submitted to periodontitis induction by placing a 4.0 silk suture ligature around the upper right second molar. The sample was divided into 4 groups, each with 10 animals: Group I: Without Periodontitis and without RACR; Group II: With Periodontitis and without RACR; Group III: With Periodontitis and with RACR; Group IV: With Periodontitis and with RACR + administration of LC, by gavage, for 30 days. Cytokine analyzes were performed by the ELISA method in gingival tissue (IL-6), intestinal tissue (IL-1β, IL-6 and IL-10) and blood (IL-1β and IL-6), the blood was also subjected to analysis biochemical (TGO, TGP, urea and creatinine) and differential leukocyte count. Fragments of the large intestine of these animals were collected and analyzed for biomarkers of oxidative stress (SOD, GSH and MDA), acetylcholinesterase (AChE) activity, and the population of Lactic Acid-Producing Bacteria in the animals' feces was counted. The use of LC adjunct to RACR resulted in a reduction in the expression of IL-6 in the gingival tissue of mice with Periodontitis (p < 0.05), for the blood inteleukins (IL-1β and IL-6), there were no differences between the groups (p > 0.05). As for intestinal cytokines, there was a reduction in the expression of IL-10 (p < 0.05), for the groups that presented Periodontitis. Regarding intestinal oxidative stress, the animals in Groups III and IV had a reduction in MDA levels (p < 0.05), for SOD and GSH, there were no significant differences between the 4 groups studied (p < 0.05 ). It is concluded that the use of LC adjunct to RACR in mice with ligation-induced periodontitis can reduce the release of IL-6 in the gingival tissue. Regarding the intestinal effects, two effects were found: The first related to the modulation of the inflammatory response, with the reduction of MDA, in the animals that received periodontal treatment. And the second related to a pro-inflammatory effect, with the reduction of IL-10 expression.

COMMITTEE MEMBERS:
Presidente - 2085732 - ANA RAFAELA LUZ DE AQUINO MARTINS
Interna - 1958729 - RUTHINEIA DIOGENES ALVES UCHOA LINS
Externo ao Programa - 1752956 - EULER MACIEL DANTAS - UFRNExterno à Instituição - FERNANDO JOSE DE OLIVEIRA NOBREGA - UERN
Externa à Instituição - SABRINA GARCIA DE AQUINO - UFPB