Banca de QUALIFICAÇÃO: JULIANA CAMPOS PINHEIRO

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : JULIANA CAMPOS PINHEIRO
DATE: 09/08/2022
TIME: 14:00
LOCAL: Plataforma Google Meet
TITLE:

BIOLOGICAL EFFECT OF LOW-LEVEL LASER IRRADIATION ON PRE-OSTEOBLASTS AND FIBROBLASTS CULTURED ON THE SURFACE OF 3D POLYMERIC SCAFFOLDS

KEY WORDS:

laser therapy; cell proliferation; biomaterials; tissue engineering.

PAGES: 72
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUMMARY:

Low-level laser irradiation (LLLI) has shown positive effects on the proliferation and viability of several cell types, but it is still little used in the field of tissue engineering. The first part of the work evaluated, through a systematic review of in vitro studies, the applicability of photobiomodulation as an auxiliary tool in tissue engineering. Of the 8373 studies initially identified based on the search criteria, ten articles met the inclusion criteria for analysis. Data obtained from most of the studies reviewed indicate that LLLI can increase the proliferation and differentiation of cells grown on the surface of biomaterials. However, the irradiation density, the exposure time, the cells cultured, and the characteristics of the scaffold must be considered in implementing more accurate and adequate photobiomodulation protocols. In the second part of the work, the effect of LLLI on the viability and proliferation of cells cultured on the surface of polymeric scaffolds produced by solution blow spinning technique will be evaluated. Cells of the MC3T3-E1 (pre-osteoblasts) and NIH-3T3 (fibroblasts) cell lines will be cultured on the surface of polylactic acid and polylactic acid/chitosan (50:50) scaffolds and subjected or not (control) to diode laser irradiation (InGaAlP; 660 nm; 30 mW; 4 J/cm²; 2min 13sec; single dose; continuous mode). The characteristics of the surfaces of the scaffolds will be evaluated before and after irradiation by scanning electron microscopy (SEM) and atomic force microscopy (AFM). Cell viability and proliferation will be assessed at intervals of 24, 48, and 72 h post-irradiation, through the MTT, Alamar blue, and Live/Dead assays, in addition to the analysis of cell-biomaterial integration by SEM. It is expected that the protocol favors the viability and proliferation of both cell types in both scaffolds, which could represent an advance in bone and skin tissue engineering research.

COMMITTEE MEMBERS:
Presidente - 2220417 - CARLOS AUGUSTO GALVAO BARBOZA
Interno - 2859541 - PEDRO PAULO DE ANDRADE SANTOS
Externo à Instituição - CARLOS EDUARDO BEZERRA DE MOURA - UFERSA