Banca de DEFESA: KATRYNE TARGINO RODRIGUES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : KATRYNE TARGINO RODRIGUES
DATE: 29/07/2022
TIME: 09:00
LOCAL: Remoto
TITLE:

EVALUATION OF THE PROBIOTIC LACTOBACILLUS RHAMNOSUS EM1107 ADJUNCT TO PERIODONTAL THERAPY IN EXPERIMENTAL MODEL OF PERIODONTITIS

KEY WORDS:

Periodontal diseases; Probiotics; Lactobacillus rhamnosus

PAGES: 52
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUBÁREA: Periodontia
SUMMARY:

Periodontal disease (PD) is characterized by a host-mediated immune-inflammatory response associated with pathogens, which compromises the protective and supporting tissues of the periodontium. It is commonly treated through non-surgical periodontal therapy in the form of scaling and root surface planing (SRP). However, in some situations, such as in the control of deep periodontal pockets, which are difficult to access and with destruction and progression of the disease over time, the practice of adjuvant therapies is indicated. In this sense, the aim of this study is to evaluate the anti-oxidant and anti-inflammatory effects of the administration of the probiotic Lactobacillus rhamnosus EM1107 (LR) as an adjunct to the basic periodontal treatment of ligature-induced periodontitis in mice. For the study, the periodontal disease induction model was used by placing a 4.0 silk ligature around the upper left second molar of mice. The animals were divided into four groups: Group I: - PD – LR - RAR; Group II: + PD – LR - RAR; Group III: + PD – LR + RAR; Group IV: + PD + LR + RAR. Biochemical analyzes of liver and kidney functions, leukocyte count, intestinal tissue concentration of glutathione (GSH), malondialdehyde (MDA) and superoxide dismutase (SOD) and expression of blood cytokines IL-1β and IL-6 were performed. The analysis of biochemical parameters revealed values above the standards in the levels of TGO/AST for groups II, III and IV. All groups maintained TGP/ALT, urea and creatinine levels within reference values. The number of leukocytes was also within the reference standards for all groups. No statistically significant differences were found between groups for tissue levels of GSH and SOD. For MDA, an increase in levels was observed in relation to the PD + RACR group, whose difference was statistically significant in relation to the positive control and negative control groups (p<0.05). There was no statistically significant difference in the concentration of inflammatory cytokines IL-1β and IL-6, despite a reduction in tissue concentrations of IL-1β in relation to the group that received the probiotic and their respective controls. There was also lower expression of the pro-inflammatory cytokine IL-6 in the PD + RACR group compared to the other groups. The use of Lactobacillus rhamnosus EM1107 in the adjuvant treatment of experimental ligature-induced periodontitis in mice was able to modulate the oxidative stress generated from the reduction in the level of malondialdehyde (MDA) and although there was no statistically significant difference, it was able to reduce the inflammatory marker IL-1β.

 

COMMITTEE MEMBERS:
Presidente - 2085732 - ANA RAFAELA LUZ DE AQUINO MARTINS
Interno - 1660087 - BRUNO CESAR DE VASCONCELOS GURGEL
Externa à Instituição - RACHEL DE QUEIROZ FERREIRA RODRIGUES - UFCG