Banca de DEFESA: JULLIANY TAVERNY SOUSA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : JULLIANY TAVERNY SOUSA
DATE: 28/07/2022
TIME: 09:00
LOCAL: DEPARTAMENTO DE ODONTOLOGIA - AUDITÓRIO
TITLE:

Immunoexpression of ING3 and cyclin D1 proteins between epithelial dysplasias and epidermoid carcinomas of the tongue.

KEY WORDS:

Oral epidermoid carcinoma. Oral Epithelial Dysplasia. Tumor biomarkers. Immunohistochemistry.

PAGES: 84
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUMMARY:

Oral squamous cell carcinoma (OSCC) is a malignant neoplasm of complex pathogenesis. In turn, oral epithelial dysplasia (OED) is an entity that, depending on its evolution, exhibits varied risks of malignant transformation to OSCC. The altered expression of tumor suppressor genes and cell cycle regulatory proteins are related to the etiopathogenesis of different malignant tumors. Among the tumor suppressor genes, the family of inhibitors of growth (ING) stands out, which prevents uncontrolled cell formation. Amid the cell cycle regulatory proteins, Cyclin D1 acts in the progression from S to G1 phase, allowing cell proliferation. The aim of the present study was to analyze the immunoexpression of ING3 and Cyclin D1 proteins in 28 OED and 25 OSCC specimens, located on the tongue, as well as morphologically evaluate and reclassify the lesions according to the criteria established by the World Health Organization (WHO) and by Kujan et al. (2006), for OED; by Almagunsh et al. (2015) for OSCC. Protein immunoexpression was quantitatively evaluated, considering the cellular location (cytoplasmic and/or nuclear for ING3 and nuclear for Cyclin D1), present in the epithelial component. Protein expression was compared between the two groups of samples, as well as with the clinical-pathological parameters of the lesions studied, through the Mann-Whitney (U) statistical tests and the Spearman correlation test, adopting a significance level of 5% for all tests considering the value of p≤ 0.05. Of the 28 cases of OED analyzed, 53.6% were high-risk lesions according to the binary model and of the 25 cases of OSCC analyzed, 48% were considered high-risk scores according to the BD model. No statistically significant associations were found between the morphological variables of the lesions studied and the clinical characteristics (p>0.05). A statistically significant difference was observed between the absence of ING3 expression and the grading of OEDs, according to the WHO (p<0.05). Regarding the expression of Cyclin D1, it was significantly higher in OED, when compared to OSCC cases (p<0.05). Nucleocytoplasmic ING3 expression was significantly lower in OSCC cases when compared to OED cases (p<0.05). Cytoplasm-restricted ING3 expression was higher in OED cases when compared to OSCC cases (p<0.05). The results of the present study suggest that, although there is no evidence of a significant relationship between the expression of proteins and the histopathological gradations of the lesions, there is a notable decrease in the nuclear expression of ING3, with an increase in the severity of the lesions, indicating an altered tumor suppressor function. of this protein in OED and OSCCs. We also highlight the increased expression of Cyclin D1 in OEDs, showing that these lesions have a significant rate of cell proliferation.

COMMITTEE MEMBERS:
Externa à Instituição - KEILA MARTHA AMORIM BARROSO - UFPB
Presidente - 344668 - LEAO PEREIRA PINTO
Interna - 1258693 - LELIA MARIA GUEDES QUEIROZ