Banca de QUALIFICAÇÃO: FRANCISCO LEONARDO DA SILVA JÚNIOR

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : FRANCISCO LEONARDO DA SILVA JÚNIOR
DATE: 20/06/2022
TIME: 10:00
LOCAL: Remoto
TITLE:

PERIODONTAL AND INTESTINAL ANTI-INFLAMMATORY EFFECT OF THE PROBIOTIC LACTOBACILUS CASEI ADJUNCTED TO PERIODONTAL THERAPY IN EXPERIMENTAL MODEL OF PERIODONTITIS.

KEY WORDS:

Periodontitis; Probiotics; Intestinal dysbiosis; Lactobacillus casei

PAGES: 36
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUBÁREA: Periodontia
SUMMARY:

Periodontitis is a chronic inflammatory condition that can influence the gut microbiota. The gold standard treatment for periodontitis is scaling and root planing, but in complex cases adjuvant therapies such as probiotics can be used. The use of this adjuvant treatment could contribute to the improvement of periodontal condition and intestinal symbiosis. The aim of this study is to evaluate the periodontal and intestinal anti-inflammatory effect of the use Lactobacillus casei (LC) adjunct to scaling and root planing in Balb/c mice with periodontitis. This research is a pre-clinical, in vivo, randomized, blinded, placebo-controlled trial, consisting of 40 male Balb/c mice. The animals will undergo periodontal disease induction by placing a 4.0 silk thread ligature around the right second molar of maxilla. The sample will be divided in 4 groups, each with 10 animals: Group I: - Periodontal Disease (PD) – Ligation (L) – SRP (Scaling and root planing); Group II: + DP – L - RAR; Group III: + DP – L + RAR; Group IV: + DP + LC + RAR. The animals in group IV will receive 30 days of gavage with LC. Analyzes will be performed with X-ray transmission computed tomography (micro-CT) of the animals' maxilla, to verify the height of the alveolar bone crest in the 2nd right molar, histomorphometric analysis of the conjunctival insertion of this tooth and immunohistochemistry for IL- 1β, IL-18, TRAP and TGF-β from periodontal tissues. Blood will still be collected from these animals for biochemical analysis (TGO, TGP, urea and creatinine) and dosage of IL-1β, CINC, IL-10 and TGF-β.  Also the intestine of these animals will be collected and submitted to analysis of IL-1β, CINC, IL-10 and TGF-β, histological by HE, to measure the infiltrate epithelial inflammatory and measure the activity of acetylcholinesterase and invertase enzymes. Feces samples will also be collected from the animals and quantified for short-chain fatty acids and LC colony formation. The data will be analyzed and the results will be submitted to analysis of variance (ANOVA) and Bonferroni test to compare the mean values between the groups. The Kruskal-Wallis test and the Dunn test will be used to compare medians.

BANKING MEMBERS:
Presidente - 2085732 - ANA RAFAELA LUZ DE AQUINO MARTINS
Interna - 2374605 - AURIGENA ANTUNES DE ARAUJO
Interna - 1958729 - RUTHINEIA DIOGENES ALVES UCHOA LINS