Banca de DEFESA: JANAÍNA LESSA DE MORAES DOS SANTOS

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : JANAÍNA LESSA DE MORAES DOS SANTOS
DATE: 21/06/2022
TIME: 14:00
LOCAL: PLATAFORMA REMOTA
TITLE:

IMMUNOHISTOCHEMICAL PROFILE OF MARKERS ASSOCIATED WITH EPITHELIAL-MESENCHYMAL TRANSITION IN BENIGN EPITHELIAL ODONTOGENIC LESIONS

KEY WORDS:

Odontogenic Cysts; Odontogenic Tumors; Immunohistochemistry, Epithelial-Mesenchymal Transition

PAGES: 89
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUBÁREA: Clínica Odontológica
SUMMARY:

Odontogenic cysts and tumors present a high percentage of the lesions that affect the maxillomandibular complex and some of them may exhibit a more aggressive clinical-biological behavior. In the epithelial-mesenchymal transition (EMT), epithelial cells lose phenotypic properties characteristic of epithelial cells and acquire properties characteristic of mesenchymal cells, including increased motility and invasiveness, through the regulation of central transcription factors and their associated pathways. Thus, the present study aimed to analyze and compare the immunohistochemical expression of proteins involved in the EMT process (Zeb1, E-cadherin, N-cadherin, and vimentin) in benign epithelial odontogenic lesions. The sample consisted of 88 cases of odontogenic lesions, comprising 28 cases of ameloblastoma (AB), 30 of odontogenic keratocyst (OK), and 30 of dentigerous cyst (DC). All specimens submitted to the immunohistochemical technique were evaluated by light microscopy and submitted to the random choice of 5 (five) fields, which were photographed at a 40x magnification. The evaluation of the expression of each marker, based on the analysis in its specific cellular compartment, was performed by multiplying the scores associated with the percentage of immunoreactive cells by the scores related to the intensity of staining. Such a result was defined as low expression or high expression, according to the methodology choosen. The associations were made using the chi-square test and the correlations through the Spearman correlation test. The significance level was set at 5% (p < 0.05). The results were statistically significant in the epidemiological variables of the lesion region, radiographic appearance, and lesion size. In turn, immunohistochemistry showed a positive and moderate correlation between nuclear Zeb1 and membrane E-cadherin, cytoplasmic Zeb1 and membrane E-cadherin, and between cytoplasmic E-cadherin and vimentin in cases of AB; a moderate positive correlation between nuclear Zeb1 and cytoplasmic vimentin, and between cytoplasmic Zeb1 and vimentin in cases of dentigerous cyst. Therefore, it was possible to conclude that Zeb1 may not act directly on the pathways responsible for the development and growth of these studied lesions, and E-cadherin, N-cadherin, and vimentin may be part of a partial EMT process that would occur in the studied benign epithelial odontogenic lesions.

BANKING MEMBERS:
Externa ao Programa - 3218128 - AMANDA KATARINNY GOES GONZAGA
Externa à Instituição - HIANNE CRISTINNE DE MORAIS MEDEIROS - FIP
Presidente - 1258693 - LELIA MARIA GUEDES QUEIROZ
Externo à Instituição - LEORIK PEREIRA DA SILVA - UFCG
Interno - 2859541 - PEDRO PAULO DE ANDRADE SANTOS