Banca de DEFESA: NARA RÉGIA DA SILVA DOMINGOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : NARA RÉGIA DA SILVA DOMINGOS
DATE: 09/11/2021
TIME: 09:00
LOCAL: PLATAFORMA VIRTUAL
TITLE:

IMMUNOEXPRESSION OF E-CADHERIN AND TWIST1 PROTEINS IN SALIVARY GLAND NEOPLASMS

KEY WORDS:

Salivary glands. Neoplasms. Epithelial-mesenchymal transition. Immunohistochemistry.

PAGES: 83
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUMMARY:

Salivary gland neoplasms present a great diversity of morphological aspects and biological behaviors, bringing diagnostic difficulties and raising interest in the scientific research of these lesions. In turn, the important role of the epithelial-mesenchymal transition (EMT) is recognized, which plays a fundamental role in tumor invasion and progression, being a necessary step for metastasis. In this process, epithelial cells lose their adhesion and polarity and acquire mesenchymal properties and increased motility, with decreased expression of epithelial biomarkers and increased expression of mesenchymal biomarkers with the involvement of several transcription factors. Among the biomarkers involved in this process are E-cadherin and Twist, which play a fundamental role in the EMT process. Therefore, this study aimed to investigate the immunoexpression of E-cadherin and Twist1 proteins in Pleomorphic Adenoma (PA), Cystic Adenoid Carcinoma (ACC) and Ex-Pleomorphic Adenoma Carcinoma (CXPA) aiming at a better understanding of the EMT process in this group of injuries. The study was cross-sectional and the sample consisted of 20 cases of PA, 20 of CAC and 10 of CXPA, in which the immunoexpression of E-cadherin and Twist1 proteins in the tumor parenchyma were analyzed. Possible differences between the expression pattern of these proteins with the clinicopathological profile of the studied lesions were investigated. A statistically significant difference was observed between a greater expression of membrane E-cadherin and the benign nature of the SGT studied (p = 0.030), however, the cytoplasmic immunoexpression of E-cadherin and Twist1 did not show significant results regarding the benign/malignant nature of the lesions (p > 0.05). Regarding the PA histopathological patterns and ACC histological subtypes, no statistically significant differences were observed between the immunoexpression of the studied proteins, similarly for the anatomical location of the cases in our sample (p > 0.05). When evaluating possible differences between the immunoexpression of E-cadherin proteins (cytoplasmic and membrane) and Twist1 with the clinical characteristics of malignant neoplasms (ACC and CXPA), statistically significant differences were evidenced between a higher expression of cytoplasmic E-cadherin in tumors of larger size (p = 0.024). E-cadherin membrane immunoexpression did not show statistically significant results for any of the clinical parameters investigated in the malignant neoplasms included in the present study (p > 0.05). Our results suggest the participation of these proteins in salivary gland neoplasms, in addition to the fact that the high cytoplasmic expression of E-cadherin may be indicative of tumors with worse clinical behavior.

BANKING MEMBERS:
Presidente - 346077 - LELIA BATISTA DE SOUZA
Interna - 1258693 - LELIA MARIA GUEDES QUEIROZ
Externo à Instituição - LEORIK PEREIRA DA SILVA - UFCG