Banca de DEFESA: HANNAH GIL DE FARIAS MORAIS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : HANNAH GIL DE FARIAS MORAIS
DATE: 25/06/2021
TIME: 09:00
LOCAL: PLATAFORMA REMOTA - GOOGLE MEET
TITLE:

IMMUNOEXPRESSION OF E-CADERIN, α-SMA, TGF-β AND SNAIL PROTEINS IN ACTINIC CHEILITIS AND SQUAMOUS CELL CARCINOMA OF THE LOWER LIP.

KEY WORDS:

Squamous cell carcinoma. Oral cancer. Epithelial-mesenchymal transition. Prognosis.

PAGES: 125
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUMMARY:

Epithelial-mesenchymal transition (EMT) is a biological process that has been widely studied in oral squamous cell carcinoma (SCC), however, it is still rarely evaluated in lip carcinogenesis. The aim of this study was to investigate the immunoexpression of E-cadherin, α-SMA, TGF-β and Snail proteins in actinic cheilitis (AC) histopathologically diagnosed as epithelial dysplasia, and in lower lip squamous cell carcinoma (LLSCC). The immunoexpression of E-cadherin, α-SMA, TGF-β and Snail was semiquantitatively analyzed in 54 cases of ACs and 49 LLSCCs. Aiming at association of immunohistochemical findings with clinicopathological variables and overall (OS) and disease-free (DFS) survival rates, cases were classified into low expression and high expression categories. No statistically significant associations were observed with any of proteins analyzed with degree of severity of epithelial dysplasia in ACs. The most aggressive morphological parameters showed significant associations with low E-cadherin membrane expression and α-SMA network/spindle immunostaining pattern, while low expression of this protein showed significance with less aggressive morphological parameters. TGF-β and Snail proteins did not reveal significant associations with morphological parameters. Regarding clinical parameters, it was found that low expression of α-SMA in stroma of tumor front and low expression of TGF-β in tumor buds were significantly associated with LLSCCs with better clinical behavior, while α-SMA network/spindle pattern significantly indicated worse clinical behavior. There were no significant associations between immunoexpression of E-cadherin and Snail with clinical parameters evaluated. Associations of protein immunoexpressions among studied lesions revealed significant results for an increase in cytoplasmic expression of E-cadherin in LLSCCs, reduction of α-SMA in ACs, reduction of TGF-β in LLSCCs and increase of Snail in ACs. Survival analysis revealed that high α-SMA expression in tumor front stroma, its network pattern and high TGF-β expression in tumor buds were significantly associated with worse survival parameters in LLSCCs. The results of present study suggest that immunoexpression of E-cadherin, α-SMA, TGF-β and Snail proteins in ACs do not indicate differences in degree of histopathological severity of these lesions, while in LLSCCs it was found that their more aggressive behavior was associated low expression of membrane E-cadherin, high expression of α-SMA and its network pattern and low expression of TGF-β in tumor buds.

BANKING MEMBERS:
Interna - 2492713 - ERICKA JANINE DANTAS DA SILVEIRA
Externa à Instituição - HELLEN BANDEIRA DE PONTES SANTOS - FACENE
Presidente - 350484 - ROSEANA DE ALMEIDA FREITAS