Banca de DEFESA: EMANUELLE LOUYDE FERREIRA DE LIMA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : EMANUELLE LOUYDE FERREIRA DE LIMA
DATE: 28/06/2021
TIME: 14:00
LOCAL: Plataforma google meet
TITLE:

ANTIFUNGAL ACTIVITY AND ANTIBIOFILM POTENTIAL OF SPONDIAS MOMBIN L. HYDROETHANOLIC EXTRACT AND ITS FRACTIONS AGAINST YEASTS OF THE GENUS CANDIDA 

KEY WORDS:

Anacardiaceae; Phytotherapy; Candidiasis, Oral.

PAGES: 55
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUBÁREA: Clínica Odontológica
SUMMARY:

The antimicrobial action of the hydroethanolic extract of Spondias mombin L. is already known in the literature against several microorganisms, including when placed against Candida genus fungi. In addition to the antifungal activity, it is essential to investigate the antibiofilm activity of substances, since these fungi can accumulate and become part of the biofilm present in the oral cavity of individuals, causing local and systemic problems, with clinical relevance. Knowing this, this study aimed to investigate the antifungal and antibiofilm action of the hydroethanolic extract (EH) of Spondias mombin L. and its fractions, against fungi of the genus Candida, of clinical importance. For this, the minimum inhibitory concentration tests were carried out using disks and the microdilution technique in microplates, minimum inhibitory concentration of adhesion, determination of the fungal death curve and antibiofilm activity of the fractions of phenolic acids, flavonoids and tannin, against the fungi Candida albicans, Candida tropicalis, Candida Krusei and Candida Glabrata, using nystatin (100,000 IU) as a positive control, in triplicate. It was seen that the antifungal and antibiofilm activity of EH and its analyzed fractions present satisfactory results, however, when we compare the fractions with EH, the latter presented better activity and when we draw the comparison between the antifungal and antibiofilm action of the tested substances and the control group, we had better results than the control group against the tested fungi. Thus, it was possible to conclude that the hydroethanolic extract with all its compounds together present superior results of antifungal and antibiofilm activity compared to the fractions of this extract tested separately and that this extract had very similar results to the control group.Palavras-chave: Anacardiaceae; Fitoterapia; Candidíase Bucal.

BANKING MEMBERS:
Presidente - 1958729 - RUTHINEIA DIOGENES ALVES UCHOA LINS
Interna - 2492713 - ERICKA JANINE DANTAS DA SILVEIRA
Interno - 1879551 - FABIO ROBERTO DAMETTO
Externo à Instituição - EDJA MARIA MELO DE BRITO COSTA - UEPB
Externo à Instituição - PEDRO HENRIQUE SETTE DE SOUZA - UPE