Banca de DEFESA: MAYRA SOUSA GOMES

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : MAYRA SOUSA GOMES
DATE: 25/06/2020
TIME: 14:00
LOCAL: PLATAFORMA GOOGLE MEETS
TITLE:

Anti-inflammatory and antioxidant activity of hydroethanolic extract of Spondias mombin leaf in an  oral mucositis experimental model

KEY WORDS:

5-fluorouracil. Oral mucositis. Spondias mombin. Oxidative stress. Inflammation.

 

PAGES: 53
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUBÁREA: Clínica Odontológica
SUMMARY:

Oral mucositis (MO) is characterized as an inflammatory condition for clinically presenting erythematous and ulcerated areas. It is the most common oral complication in patients undergoing chemotherapy and / or radiotherapy, and its treatment aims to relieve painful symptoms and induce the injury repair process. Spondias mombin is a plant widely used in northeastern Brazil and its leaves are used in folk medicine to treat inflammation in the mouth and throat. Aim: To evaluate the antioxidant and anti-inflammatory potentials of the hydroethanolic extract of the leaf of Spondias mombin (HESM) in an experimental model of oral mucositis. Methods: A pre-clinical, in vivo, blind, randomized and controlled trial was performed. The sample consisted of 42 male hamsters, divided into 6 groups: Saline (S), Mechanical trauma (TM), 5 fluorouracil / Mechanical trauma (5-FU / TM) and experimental HESM doses of 50, 100 and 200 mg / kg, for ten days. The animals were submitted to biochemical analyzes and the samples of pouches of the cheek mucosa were subjected to macroscopic, histopathological and immunohistochemical analyzes, with Cox-2, iNOS, NF-kB p50 NLS and MMP-2 markers. The levels of IL-1β and TNF-α were analyzed by ELISA immunoreaction and the levels of superoxide dismutase (SOD), glutathione (GSH) and malondialdehyde (MDA) were submitted to spectroscopic analysis. Results: The HESM-treated group at a dose of 200 mg / kg showed the best healing effect, showing no evidence of ulceration in macroscopic analysis (p <0.05). Histopathological analysis of this group showed reepithelialization, a mild mononuclear inflammatory infiltrate and absence of a bleeding and edema score of 1 (1-1) (p <0.05), in addition to a large amount of collagen fibers. Reduced immunoexpression of Cox-2, iNOS, NF-kB p50 NLS and MMP-2; decreased levels of SOD (p <0.05), MDA (p <0.001), IL-1β (p <0.05) and TNF-α (p <0.001); and an increase in GSH levels (p <0.01) were also observed in this same group. Conclusion: HESM (200 mg / kg) reduced oxidative stress and inflammation in oral mucositis induced by 5-fluorouracil in hamsters.

BANKING MEMBERS:
Interna - 2085732 - ANA RAFAELA LUZ DE AQUINO MARTINS
Interno - 2220417 - CARLOS AUGUSTO GALVAO BARBOZA
Externo à Instituição - EDJA MARIA MELO DE BRITO COSTA - UEPB
Externa à Instituição - MARIA CECILIA AZEVEDO DE AGUIAR - UNIFACEX
Presidente - 1958729 - RUTHINEIA DIOGENES ALVES UCHOA LINS