Banca de DEFESA: JOAQUIM FELIPE JUNIOR

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : JOAQUIM FELIPE JUNIOR
DATE: 20/02/2020
TIME: 15:00
LOCAL: SALA III DO DEPARTAMENTO DE ODONTOLOGIA
TITLE:

MORPHOLOGICAL AND IMMUNOHISTOCHEMICAL EVALUATION OF EPITELIAL PROLIFERATIVE POTENTIAL OF AGGRESSIVE ODONTOGENIC CYSTIC INJURIES

KEY WORDS:

odontogenic cysts; cyclin D1; EGFR; SOX 2.

PAGES: 66
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUMMARY:

INTRODUCTION: Odontogenic cysts are known for their growth potential and bone resorption of gnathic bones, ranging from indolent to aggressive, regardless of their histogenesis, linked to the epithelial, ectomesenchymal and / or mesenchymal remnants of odontogenesis. Most of these lesions have controversial pathogenesis, which is why they stimulate numerous investigations on the possible relationship between the odontogenic epithelium and the development of these lesions. OBJECTIVE: To analyze the different patterns of proliferation of odontogenic epithelium in odontogenic cystic lesions with more aggressive behavior, through the analysis of the epidermal growth factor (EGFR) immunoexpression and the SOX 2 transcription pathway in the control of the cell cycle phases (cyclin D1 ) in aggressive odontogenic lesions. MATERIALS AND METHODS: Sectional study with a non-probabilistic sample for convenience consisting of 31 cases, 10 cases of recurrent odontogenic keratocysts (COs) isolated, 10 cases of botryoid odontogenic cysts (BOC) and 4 cases of odontogenic cysts glandular (COG). RESULTS: When analyzing statistically with the Mann-Whitney and Kruskal-Wallis tests; P <0.05 was considered significant. The highest expression of positive cells for cyclin D1 was observed in the suprabasal layer of odontogenic keratocysts (P <0.001) and in the basal and suprabasal layers of COGs (P <0.001), and portions of COBs (P> 0.001). There was no statistical difference between recurrent and non-recurring COs. In addition, all cases of COs, COBs and COGs were positive for EGFR in all layers of the cystic epithelial lining. COBs and COGs showed negativity for SOX 2 while COs found high expression of SOX 2 in the suprabasal layer. CONCLUSIONS: Cyclin D1 implies cell cycle disorders in G1-S phases, reflecting the aggressiveness of COs and COGs. The overexpression of EGFR demonstrated in COs, COBs and COGs suggests that the combination of cytoplasmic and membranous cytoplasmic is indicative of the proliferation potential of the epithelial lining. The high standard of SOX 2 expression in CO can also explain the aggressive nature of the lesion and are responsible for the presence of numerous child cysts responsible for its high rate of recurrence. The CSC subpopulation presents itself as a reason for recurrence to COs, due to the self-renewal and pluripotency properties of these cells.

BANKING MEMBERS:
Interno - 1258707 - ANTONIO DE LISBOA LOPES COSTA
Presidente - 350485 - HEBEL CAVALCANTI GALVAO
Externo à Instituição - MANUEL ANTONIO GORDON NUNEZ - UEPB