Banca de DEFESA: CAROLINA MARIA CAMPOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : CAROLINA MARIA CAMPOS
DATE: 19/12/2019
TIME: 14:00
LOCAL: AUDITÓRIO DO DEPARTAMENTO DE ODONTOLOGIA
TITLE:

IMMUNOHISTOCHEMICAL EXPRESSION OF ING1 AND ING2 PROTEINS IN BENIGN EPITELIAL DENTAL INJURIES

KEY WORDS:

odontogenic cysts. odontogenic tumors. Tumor suppressor gene. Inhibitor of Growth Protein 1. Immunohistochemistry.

PAGES: 85
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUMMARY:

Epithelial odontogenic lesions constitute a heterogeneous group of lesions whose biological behavior is still under investigation. originated from epithelial and / or ectomesenchymal tissues of the dental organ. Defects in cell cycle regulation may be involved in the development and progression of these lesions. INGs (inhibitors of growth) proteins play an important role in controlling cell cycle-related mechanisms. The aim of this study was therefore to investigate the expression of ING1 and ING2, to better understand the possible role of these proteins in ameloblastoma (AMB) (n = 20), adenomatoid odontogenic tumor (TOA) (n = 20), odontogenic keratocyst ( CO) (n = 20) and dental follicle (FD) (n = 10). The immunohistochemical expression of ING1 and ING2 was quantitatively evaluated by a single evaluator. In five fields of each lesion, positive and negative cells in the nucleus and / or cytoplasm were quantified, establishing the percentage of positive cells in relation to the total number of cells. Data were submitted to statistical analysis using the Kruskal-Wallis (KW), Dunn and Spearman (r) tests, with the significance level set at 5% (p <0.05). There was a significant difference between ING1 nuclear labeling in the analyzed groups. AMBs and COs exhibited significantly lower ING1 expression than FDs. There was a difference in the concomitant nucleus / cytoplasmic labeling of ING1, with a significantly higher expression in AMB cases when compared to the other groups (p <0.05). For the ING2 protein, there was a difference in nuclear labeling between the analyzed groups (p = 0.0001), and a significantly lower expression in the cases of AMBs, TOAs and COs when compared to the FDs (p <0.05). When comparing groups two by two in the expression nucleus / cytoplasmic, it was observed that the group of AMBs and COs showed a higher expression than TOA (p = 0.002 and p = 0.0001). For all groups studied, there was a negative correlation of ING1 and ING2 labeling in the nucleus / cytoplasm with the cytoplasm restricted labeling. The results of this study suggest the participation of ING1 and ING2 proteins in the pathogenesis of the lesions studied and the immunostaining of ING1 and ING2 in AMBs and COs indicates that these proteins contribute to the more aggressive biological behavior of these two lesions when compared to TOA.

BANKING MEMBERS:
Externa à Instituição - JAMILE MARINHO BEZERRA DE OLIVEIRA MOURA - UERN
Presidente - 1258693 - LELIA MARIA GUEDES QUEIROZ
Externa ao Programa - 3121806 - MARIA LUIZA DINIZ DE SOUSA LOPES