Banca de DEFESA: CAROLINA LIMA DOS SANTOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : CAROLINA LIMA DOS SANTOS
DATE: 31/03/2026
TIME: 09:00
LOCAL: GOOGLE MEET
TITLE:

NANOPARTICLES CONTAINING ASTAXANTHIN-PIGMENTED OIL FROM SHRIMP (Litopeneaus vannamei) WASTE MEAL: EVALUATION OF CYTOTOXICITY AND BIOACTIVE POTENTIAL IN VITRO

KEY WORDS:

Shrimp; Waste; Natural pigment; Nanotechnology; Characterization.

PAGES: 96
BIG AREA: Ciências da Saúde
AREA: Nutrição
SUMMARY:

In Brazil, processing Litopenaeus vannamei shrimp generates approximately 50–60% waste, which is rich in astaxanthin, a carotenoid with high bioactive potential and applications in the food industry. However, this pigment presents technological challenges due to its low solubility in aqueous matrices and its instability under conditions
encountered in food processing and storage. Therefore, encapsulation is an alternative to preserve these bioactive compounds. In this context, the objective of this study was to produce and characterize nanoparticles containing astaxanthin-pigmented oil extracted from shrimp waste meal, and to evaluate their cytotoxicity and functional properties in vitro. The nanoparticles were produced using the oil-in- water emulsification technique, with porcine gelatin and isolated soy protein as encapsulating agents and Tween 20 as a surfactant. Three formulations were obtained: pigmented oil and porcine gelatin (EAG); Pigmented oil and a combination of porcine gelatin and isolated soy protein in the proportions 2:2 w/w (EAGS2:2) and 3:1 w/w (EAGS3:1) were evaluated. Characterization was performed by Scanning Electron Microscopy (SEM), Dynamic Light Scattering (DLS), and Fourier Transform Infrared Spectroscopy (FTIR). Encapsulation efficiency, thermal properties, cytotoxicity in CHO-K1 and HepG2 cells, antioxidant capacity, and inhibition of glucose metabolism enzymes were also assessed. The micrographs showed particles with sizes between 100 and 200 nm, spherical shape, and smooth surface. The average sizes obtained by DLS were 205±10.04 nm for EAG (PDI 0.16±0.05), 154±12.05 nm for EAGS2:2 (PDI 0.33±0.134), and 76.70±14.05 nm for EAGS3:1 (PDI 0.015±0.02). FTIR showed an enhancement of the O–H and C–H stretching bands (3310, 3300, and 3310 cm−1), indicating chemical interactions between the pigmented oil and the encapsulating materials. The formulations showed high encapsulation efficiency of the pigmented oil, with values of 98.8±0.08% (EAG), 98.6±0.67% (EAGS2:2), and 98.7±0.12% (EAGS3:1). The astaxanthin incorporation efficiency was 56.997±0.01% (EAG), 57.296±0.00% (EAGS2:2), and 56.603±0.02% (EAGS3:1). In the cytotoxicity evaluation, the pigmented oil and nanoformulations did not show a cytotoxic effect on HepG2 cells,
with MTT reduction between 98.68±0.12% and 112.39±1.35% at 24 h and between 101±1% and 109±2% at 72 h. In CHO-K1 cells, greater sensitivity was observed, with viability of 79±7% and 81±7% at 24 h for EAGS2:2 and EAGS3:1 (1000 μg/mL). After 72 h, viability decreased to 62±8% (oil), 61±8% (EAG), 56±3% (EAGS3:1), and 33±6% (EAGS2:2). In the bioactivity assessment, the pigmented oil (41.03±6.44) and the nanoformulations EAGS2:2 (44.17±3.93) and EAGS3:1 (34.1±1.87) showed an effect on amyloglucosidase. For α-amylase, only EAGS2:2 (41.1±14.3) and EAGS3:1 (35.8±20.48) showed significant inhibition (p < 0.05). The EAGS2:2 formulation showed the highest antioxidant potential by ABTS (7.51±0.067 μmol Trolox/g), while EAG showed the
highest reducing power (81.02±4.42%). During simulated digestion, greater fatty acid release was observed during the intestinal phase. Among the formulations evaluated, EAGS2:2 stood out for its greater bioactive potential, but it exhibited greater cytotoxicity in CHO-K1 cells at high concentrations and under prolonged exposure. Thus, nanoencapsulation proved to be a promising approach for developing new food ingredients with functional potential.

 

 

COMMITTEE MEMBERS:
Externa à Instituição - ANNA BEATRIZ SANTANA LUZ - UFRB
Interna - 2378605 - CRISTIANE FERNANDES DE ASSIS
Externa à Instituição - MAYARA SANTA ROSA LIMA - UFRB
Interna - 2275877 - THAIS SOUZA PASSOS