Banca de DEFESA: LETICIA ALVES BORGES E PIRES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : LETICIA ALVES BORGES E PIRES
DATE: 03/09/2025
TIME: 09:00
LOCAL: Departamento de Morfologia- CB
TITLE:

Hepatic evaluation of diabetic mice treated with hydroalcoholic extract from the peel of Spondias tuberosa Arruda fruit

KEY WORDS:

Experimental diabetes mellitus. Spondias tuberosa. Liver. Morphology.

PAGES: 62
BIG AREA: Ciências Biológicas
AREA: Morfologia
SUBÁREA: Histologia
SUMMARY:

Type 1 diabetes mellitus (DM1) occurs due to a failure in insulin secretion caused by autoimmune destruction of pancreatic beta cells, leading to hyperglycemia and systemic impairment. In this condition, the liver, which is commonly affected, presents with various morphofunctional changes and accumulation of reactive oxygen species (ROS). Due to its multifactorial etiology, the pathophysiology of DM is not completely understood, which limits the specificity of pharmacological therapies. Thus, alternative treatments based on medicinal plants are potentially useful for treating DM. The species Spondias tuberosa Arruda (“umbuzeiro”) has been popularly used for the treatment of digestive diseases, infections, and diabetes, but has been little explored scientifically. Considering its pharmacological potential, the objective of this research is to evaluate the possible hepatoprotective effect of the hydroalcoholic extract of the fruit peel of Spondias tuberosa Arruda (ExSt) in diabetic animals. Forty male C57BL/6 mice were used. DM was induced by streptozotocin (65 mg/kg i.p.), and ExSt was administered therapeutically or preventively for seven consecutive weeks orally at a dose of 100 mg/kg. Four groups were established: CT: Control group; DM: Diabetic group; DM/TER: Diabetic/therapeutic group; DMPRE: Diabetic/preventive group. Serum and liver samples were sent for biochemical, morphological, and molecular analysis. The results showed that diabetic animals experienced weight loss, increased water and feed consumption, and morphological changes in liver injury, with ExSt treatment able to attenuate necrosis and inflammation. Furthermore, ExSt was able to reduce ALT levels in the DMTER group, but not glycemic levels. It modulated SOD activity, reduced ROS, and increased hepatic expression of SOD-1, PI3Kp110b, and AKT-1. In the in vitro evaluation using HepG2 cells in a hyperglycemic microenvironment, ExSt (100 ug/mL) showed no cytotoxicity and was able to modulate oxidative stress. This study shows that although ExSt did not maintain normoglycemia in experimental DM, it was able to improve some morphological and molecular parameters of liver damage. However, further studies are needed to better understand the protective mechanism of action of ExSt in DM. Furthermore, this study helped to understand the pathogenesis of DM, in addition to encouraging the search for new forms of treatment, valuing natural products derived from Brazilian flora.

COMMITTEE MEMBERS:
Presidente - 1714418 - KARINA CARLA DE PAULA MEDEIROS
Interno - 2329140 - RAIMUNDO FERNANDES DE ARAUJO JUNIOR
Interna - 1720860 - VANESSA DE PAULA SOARES RACHETTI
Externa à Instituição - CRISTIANE DAMAS GIL - UNIFESP