Banca de DEFESA: CECILIA PAULINO CASSIANO DA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : CECILIA PAULINO CASSIANO DA SILVA
DATE: 07/06/2024
TIME: 09:00
LOCAL: Departamento de Morfologia- CB
TITLE:

HEPATIC AND PANCREATIC EVALUATION OF DIABETIC ANIMALS INDUCED BY STREPTOZOTOCIN TREATED WITH SPONDIAS TUBEROSA RUDE EXTRACT

KEY WORDS:

Diabetes; Spondias tuberosa; morphology, liver, pancreas

PAGES: 46
BIG AREA: Ciências Biológicas
AREA: Morfologia
SUBÁREA: Histologia
SUMMARY:

Diabetes mellitus (DM) is a chronic metabolic disorder characterized by persistent hyperglycemia, resulting from deficiency in insulin production by the pancreas and/or its action, leading to long-term complications. Pharmacological therapy for DM faces limitations due to the high number of side effects. Alternative treatments based on natural products have potential in the treatment of DM. The species Spondias tuberosa Arruda, known as umbuzeiro, has been widely used in traditional medicine to treat digestive diseases, infections and diabetes. Despite its frequent use, there are few studies on its benefits. However, a recent study demonstrated its antihyperglycemic activity. Considering the easy access to this species in the region and its pharmacological potential, the objective of this work is to evaluate the antidiabetogenic effect of Spondias tuberosa Arruda, in diabetic rats induced by streptozotocin, including clinical, biochemical, morphological analyses, and evaluation of possible markers involved in the therapeutic effect of the species under study. Fifty-two Wistar rats were used, and diabetes mellitus was induced by streptozotocin (40mg/kg i.p.). The groups were divided into 5: GC (control group animals), DM (animals with experimental diabetes), EX (non-diabetic animals treated with S. tuberosa extract), DMEX (animals with experimental diabetes treated with S. tuberosa extract ) and DMIN (animals with experimental diabetes treated with insulin). S. tuberosa extract (500 mg/kg, p.o.) and NPH insulin (10UI s.c.) were administered daily for 30 days after the onset of diabetes mellitus. The parameters analyzed showed that there was an increase in water intake, feed consumption and urinary volume, with weight loss in diabetic animals, a significant decrease in AST (aspartate aminotransferase) in the group treated with the extract, morphology and morphometry of the pancreas with considerable damage. representative samples showing atrophy and vacuoles in animals with DM and improvement in animals treated with insulin and extract. In the evaluation of liver morphology, there was an improvement in glycogen deposition in both extract and insulin treatments. In the evaluation of reactive oxygen species, it was observed that DM increased the immunofluorescence of this parameter, as well as the SOD enzyme by immunohistochemistry, however the extract was unable to reverse it.   Given these results, it can be concluded that the extract reduced the histopathological and morphometric changes in liver and pancreatic tissue, even without managing to alleviate hyperglycemia. Investigating this plant will contribute to a better understanding of the complex pathophysiology of DM1 and will encourage research into new therapeutic or complementary alternatives based on medicinal plants from the rich Brazilian flora.

COMMITTEE MEMBERS:
Presidente - 1714418 - KARINA CARLA DE PAULA MEDEIROS
Interno - 2329140 - RAIMUNDO FERNANDES DE ARAUJO JUNIOR
Externa à Instituição - Islania Giselia Albuquerque Gonçalves - UFPB