Banca de QUALIFICAÇÃO: THAMIRES BARRETO SANCHO

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : THAMIRES BARRETO SANCHO
DATE: 10/06/2024
TIME: 09:00
LOCAL: REMOTO: https://meet.google.com/xjh-oqoz-ebc
TITLE:

COMPARISON OF MORPHINE AND TRAMADOL ON PROLIFERATION, APOPTOSIS, AND LUNG METASTASIS OF MELANOMA TUMOR CELLS IN VITRO AND IN VIVO

KEY WORDS:

Melanoma, Morphine, Tramadol, Opioides, Tumor Growth

PAGES: 23
BIG AREA: Ciências da Saúde
AREA: Medicina
SUMMARY:

Tramadol and morphine are analgesic drugs used to manage cancer pain. Morphine, depending on factors, can inhibit or stimulate the growth of melanoma cells, but there is no data on tramadol. Therefore, the aim of the present study was to compare the effects of morphine and tramadol on melanoma cells in vitro and in vivo. The two drugs, in a dose-dependent manner, were cytotoxic to B16F10 melanoma cells, reaching a maximum effect (80%) at 5.0 μM. However, at this concentration, morphine (49%) induced more apoptosis in cells than tramadol (37%). In the clonogenic test, tramadol (0.5 μM) inhibited the number of colonies formed by 80%, while morphine (0.5 μM) inhibited the formation of colonies by 100%. When administered at clinical doses, both morphine and tramadol significantly (p<0.05) decreased the formation of metastases in mice. However, morphine showed a greater reduction in lung tumorigenicity compared to tramadol (p<0.05). The association of tramadol at a clinical dose (2.00 μM) with cisplatin 0.0312 μM potentiated the inhibition of cell proliferation compared to cisplatin alone (p<0.05). In summary, morphine was more effective in inhibiting the formation of lung metastasis compared to tramadol. At clinical doses, tramadol potentiated the in vitro antitumor effect of cisplatin. However, further experiments are needed to determine whether clinical doses of morphine have an antitumor effect and whether tramadol has the potential to replace morphine in antitumor and pain management in patients with melanoma cancer.

COMMITTEE MEMBERS:
Presidente - 346138 - ALDO DA CUNHA MEDEIROS
Externa à Instituição - AMALIA CINTHIA MENESES DO REGO - UnP
Externo à Instituição - ITALO MEDEIROS DE AZEVEDO - UFRN