Banca de QUALIFICAÇÃO: RAFAELA ALCINDO SILVA DE SOUSA FÉ

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : RAFAELA ALCINDO SILVA DE SOUSA FÉ
DATE: 19/12/2025
TIME: 14:00
LOCAL: Laboratório de Farmacologia II Departamento de Biofísica e Farmacologia
TITLE:

Biocompatibility of a new Portland cement formulation associated with niobium: an in vivo study.

KEY WORDS:

Biocompatibility; Portland cement; Niobium; Endodontics

PAGES: 48
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUMMARY:

Introduction: Maintaining pulp vitality is one of the main objectives of conservative endodontic therapy. For the success of this procedure, the use of biocompatible materials is essential, and Portland cement associated with niobium oxide (Nb₂O₅) has emerged as a promising alternative to conventional materials such as calcium hydroxide (HC) and MTA. Objective: To evaluate the biological performance of the F6 cement, composed of Portland cement, calcium sulfate, and Nb₂O₅, using a subcutaneous implantation model in rats. Material and Methods: Polyethylene tubes containing F6, calcium hydroxide (HC), and an empty tube (CN) were implanted into the subcutaneous tissue of rats. The samples were processed for Raman spectroscopy, X-ray fluorescence (XRF), Hematoxylin and Eosin (HE), Von Kossa staining, polarized light microscopy, Picrosirius staining, immunohistochemistry, and RT-PCR analyses. Statistical analysis was performed using the Kruskal–Wallis and Mann–Whitney tests, with a significance level of 5%. Partial Results: XRF analysis showed that F6 exhibited calcium accumulation near the implant and a localized concentration of Nb₂O₅, while Raman spectroscopy revealed bands compatible with calcium compounds and collagenous matrix. Histological analysis (HE) showed that F6 exhibited significantly less inflammation on day 7 compared with HC (p < 0.05). Intragroup comparisons revealed a significant reduction in inflammation over time in all groups (p < 0.05). Polarized light and Von Kossa staining demonstrated birefringent crystalline deposits and dark precipitates, respectively, in F6 and HC, which were absent in CN. RT-PCR revealed a statistically significant increase in WNT expression in the F6 group compared with the negative control (p < 0.01). Final Considerations: These findings indicate that F6 exhibits favorable biological behavior, promotes mineral formation, and stands out as a potential material for pulp capping applications, supporting the continuation of complementary analyses.

COMMITTEE MEMBERS:
Presidente - 2374605 - AURIGENA ANTUNES DE ARAUJO
Interno - 2859541 - PEDRO PAULO DE ANDRADE SANTOS
Externa ao Programa - 2477216 - NAISANDRA BEZERRA DA SILVA FARIAS - UFRN