Banca de QUALIFICAÇÃO: LEONARDO MAGALHÃES CARLAN

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : LEONARDO MAGALHÃES CARLAN
DATE: 27/08/2024
TIME: 14:30
LOCAL: Departamento de Odontologia
TITLE:

EVALUATION OF THE ROLE OF GLI-1 AND SNAIL IN PROMOTING EPITHELIAL-MESENCHYMAL TRANSITION IN SPECIMENS OF PROLIFERATIVE VERRUCOUS LEUKOPLASIA AND HISTOPATHOLOGICAL VARIANTS OF SQUAMOUS CELL CARCINOMA IN THE HEAD AND NECK REGION

KEY WORDS:

Squamous Cell Carcinoma. Epithelial-Mesenchymal Transition. Transcription Factors. carcinogenesis.

PAGES: 69
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUMMARY:

The occurrence of tumor progression depends on the acquisition of an aggressive phenotype by neoplastic cells, which increases cell motility and treatment resistance, favoring tumor progression. Molecular pathways, such as Hedgehog, promote epithelial-mesenchymal transition (EMT), where the transcription factor Gli-1 induces Snail1 overexpression, leading to the loss of E-cadherin and N-cadherin expression. Given that squamous cell carcinoma exhibits histological variants with different invasive patterns and prognoses, and that proliferative verrucous leukoplakia has the highest cancer evolution rate, it is important to investigate the role of Gli-1 in these lesions. The proposed study aims to analyze Gli-1 expression and its correlation with EMT markers (Snail1, E-cadherin, and N-cadherin) in biopsy specimens of proliferative verrucous leukoplakia, squamous cell carcinoma, verrucous carcinoma, and spindle cell squamous carcinoma of the head and neck. This study was approved by the Ethics Committee (approval number 74204623.4.0000.5537) and will be conducted as a retrospective, observational, and cross-sectional study, using immunohistochemistry, RT-PCR, and Western Blot techniques to quantify Gli-1, E-cadherin, N-cadherin, and Snail1 proteins. Possible associations between the expression of these proteins and lesion diagnosis, morphological characteristics, and the presence of stromal and inflammatory cells will be investigated. The results are expected to contribute to a better understanding of the molecular events involved in the malignant transformation of lesions with evolutionary potential, such as proliferative verrucous leukoplakia, and to assess whether differences in the expression of these factors are related to the histological variants of squamous cell carcinoma.

COMMITTEE MEMBERS:
Presidente - 350485 - HEBEL CAVALCANTI GALVAO
Interno - 2859541 - PEDRO PAULO DE ANDRADE SANTOS
Interna - 350484 - ROSEANA DE ALMEIDA FREITAS