ALTERNATIVE THERAPIES IN THE CONTROL OF SUPERINFECTIOUS AND OPPORTUNISTIC MICROORGANISMS IN THE ORAL ENVIRONMENT
Alternative therapies; Microbiology; Antimicrobial photodynamic therapy; Methylene blue; Lactobacillus rhamnosus; Spondias mombin L.
Opportunistic microorganisms are those that establish infectious processes when the host's immune conditions allow. Within this group, we have superinfectious microorganisms that proliferate rapidly when part of the resident microbiota is suppressed and are known for their resistance to most antimicrobials. In this regard, the increasing prevalence and complexity of multi-drug resistant microorganisms have prompted the search for alternative therapies capable of effectively addressing emerging drug resistance. Therefore, this study aims to comparatively evaluate the antimicrobial action in vitro of antimicrobial photodynamic therapy (methylene blue), phytotherapy (Spondias mombin L. at a concentration of 500 µm/ml), and probiotics (Lactobacillus rhamnosus) in controlling superinfectious and opportunistic microorganisms in the oral environment (Pseudomonas aeruginosa, Acinetobacter baumannii, and Klebsiella pneumoniae). To achieve this, we will investigate the Inhibitory Activity of Microbial Growth in their planktonic and Mixed Culture forms, Evaluation of Anti-adhesive Activity Bactericidal Kinetics, and Antibiofilm Activity in mono and multispecies biofilms, using 0.12% chlorhexidine digluconate as a control, in triplicate. In terms of statistical analysis, in addition to descriptive interpretation, the Two-Way ANOVA and Tukey Test the will be applied in this study.